skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Microfluidics and microacoustics for miniature flow cytometry.


No abstract prepared.

 [1]; ; ;
  1. (Los Alamos National Laboratories, Los Alamos, NM)
Publication Date:
Research Org.:
Sandia National Laboratories
Sponsoring Org.:
OSTI Identifier:
Report Number(s):
TRN: US200713%%70
DOE Contract Number:
Resource Type:
Resource Relation:
Conference: Proposed for presentation at the NSTI Nanotech 2007 held May 20-24, 2007 in Santa Clara, CA.
Country of Publication:
United States

Citation Formats

Kaduchak, Gregory, Brener, Igal, Klem, Paul Gilbert, and Ravula, Surendra K. Microfluidics and microacoustics for miniature flow cytometry.. United States: N. p., 2006. Web.
Kaduchak, Gregory, Brener, Igal, Klem, Paul Gilbert, & Ravula, Surendra K. Microfluidics and microacoustics for miniature flow cytometry.. United States.
Kaduchak, Gregory, Brener, Igal, Klem, Paul Gilbert, and Ravula, Surendra K. Sun . "Microfluidics and microacoustics for miniature flow cytometry.". United States. doi:.
title = {Microfluidics and microacoustics for miniature flow cytometry.},
author = {Kaduchak, Gregory and Brener, Igal and Klem, Paul Gilbert and Ravula, Surendra K.},
abstractNote = {No abstract prepared.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 2006},
month = {Sun Jan 01 00:00:00 EST 2006}

Other availability
Please see Document Availability for additional information on obtaining the full-text document. Library patrons may search WorldCat to identify libraries that hold this conference proceeding.

Save / Share:
  • Abstract not provided.
  • Abstract not provided.
  • ABSTRACT The potential for the use of biological agents by terrorists is a real threat. Two approaches for detection of biological species will be described: 1) The use of microbead arrays for multiplexed flow cytometry detection of cytokines and botulinum neurotoxin simulant, and 2) a microfluidic platform for capture and separation of different size superparamagnetic nanoparticles followed by on-chip fluorescence detection of the sandwich complex. The methods and automated fluidic systems used for trapping functionalized microbeads will be described. This approach allows sample, assay reagents, and wash solutions to be perfused over a micro-column of beads, resulting in faster andmore » more sensitive assays. The automated fluidic approach resulted in up to five-fold improvements in assay sensitivity/speed as compared to identical assays performed in a typical manual batch mode. A second approach for implementing multiplexed bead-based assays without using flow cytometry detection is currently under development. The goal of the microfluidic-based approach is to achieve rapid (<20 minutes), multiplexed (> 3 bioagents) detection using a simple and low-cost, integrated microfluidic/optical detection platform. Using fiber-optic guided laser-induced fluorescence, assay detection limits were shown to be in the 100’s of picomolar range (10’s of micrograms per liter) for botulinum neurotoxin simulant without any optimization of the microfluidic device or optical detection approach. Video taping magnetic nanoparticle capture and release was used to improve understanding of the process and revealed interesting behavior.« less
  • Abstract not provided.
  • Flow cytometry has enabled measurement of the kinetics of formyl peptide stimulated neutrophil aggregation and its dependence on the CD11b/CD18 adhesion molecule. The authors are currently measuring aggregation of neutrophils in whole blood using flow cytometry. Fresh whole blood samples were kept at 4C and stained with LDS-751 a vital nucleic stain. Cytometric detection of neutrophil aggregation in whole blood at 37C was achieved by thresholding on LDS-751 fluorescence and then gating on forward and right angle light scatter. Aggregation was up to 10 times more efficient in whole blood than in purified cells, despite the fact that the numbermore » of CD11b/CD18 sites was upregulated 5-10 fold in elutriated neutrophil preparations. The time course of whole blood aggregation was often irreversible as compared to elutriated cells. Aggregation was only partially blocked by preincubation with concentrations of antibody to the CD18 integrin effective in blocking aggregation in elutriated cells. Further study is needed to distinguish between the contributions of other cell types, as well as the activity and number of CD11b/CD18 adhesive sites on the kinetics and efficiency of neutrophil collisions in whole blood.« less