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Title: Quantitative oral dosing of water soluble and lipophilic contaminants in the Japanese medaka (Oryzias latipes)

Abstract

Quantitative oral dosing in fish can be challenging, particularly with water soluble contaminants, which can leach into the aquarium water prior to ingestion. We applied a method of bioencapsulation using newly hatched brine shrimp (Artemia franciscana) nauplii to study the toxicokinetics of five chlorinated and brominated halogenated acetic acids (HAAs), which are drinking water disinfection by-products. These results are compared to those obtained in a previous study using a polybrominated diphenyl ether (PBDE-47), a highly lipophilic chemical. The HAAs and PBDE-47 were bioencapsulated using freshly hatched A. franciscana nauplii after incubation in concentrated solutions of the study chemicals for 18 h. Aliquots of the brine shrimp were quantitatively removed for chemical analysis and fed to individual fish that were able to consume 400–500 nauplii in less than 5min. At select times after feeding, fish were euthanized and the HAA or PBDE-47 content determined. The absorption of HAAs was quantitatively similar to previous studies in rodents: rapid absorptionwith peak body levels occurringwithin 1–2 h, then rapidly declining with elimination half-life of 0.3–3 h depending on HAA. PBDE-47 was more slowly absorbed with peak levels occurring by 18 h and very slowly eliminated with an elimination half-life of 281 h.

Authors:
; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
900919
Report Number(s):
PNWD-SA-7669
TRN: US200713%%23
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 145(1):86-95; Journal Volume: 145; Journal Issue: 1
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; ORGANIC CHLORINE COMPOUNDS; ORGANIC BROMINE COMPOUNDS; ABSORPTION; ARTEMIA; INGESTION; WATER POLLUTION; SOLUBILITY; STERILIZATION; DRINKING WATER; BY-PRODUCTS

Citation Formats

Schultz, Irv, Reed, Stacey M., Pratt, Amanda V., and Skillman, Ann D. Quantitative oral dosing of water soluble and lipophilic contaminants in the Japanese medaka (Oryzias latipes). United States: N. p., 2007. Web. doi:10.1016/j.cbpc.2006.09.006.
Schultz, Irv, Reed, Stacey M., Pratt, Amanda V., & Skillman, Ann D. Quantitative oral dosing of water soluble and lipophilic contaminants in the Japanese medaka (Oryzias latipes). United States. doi:10.1016/j.cbpc.2006.09.006.
Schultz, Irv, Reed, Stacey M., Pratt, Amanda V., and Skillman, Ann D. Thu . "Quantitative oral dosing of water soluble and lipophilic contaminants in the Japanese medaka (Oryzias latipes)". United States. doi:10.1016/j.cbpc.2006.09.006.
@article{osti_900919,
title = {Quantitative oral dosing of water soluble and lipophilic contaminants in the Japanese medaka (Oryzias latipes)},
author = {Schultz, Irv and Reed, Stacey M. and Pratt, Amanda V. and Skillman, Ann D.},
abstractNote = {Quantitative oral dosing in fish can be challenging, particularly with water soluble contaminants, which can leach into the aquarium water prior to ingestion. We applied a method of bioencapsulation using newly hatched brine shrimp (Artemia franciscana) nauplii to study the toxicokinetics of five chlorinated and brominated halogenated acetic acids (HAAs), which are drinking water disinfection by-products. These results are compared to those obtained in a previous study using a polybrominated diphenyl ether (PBDE-47), a highly lipophilic chemical. The HAAs and PBDE-47 were bioencapsulated using freshly hatched A. franciscana nauplii after incubation in concentrated solutions of the study chemicals for 18 h. Aliquots of the brine shrimp were quantitatively removed for chemical analysis and fed to individual fish that were able to consume 400–500 nauplii in less than 5min. At select times after feeding, fish were euthanized and the HAA or PBDE-47 content determined. The absorption of HAAs was quantitatively similar to previous studies in rodents: rapid absorptionwith peak body levels occurringwithin 1–2 h, then rapidly declining with elimination half-life of 0.3–3 h depending on HAA. PBDE-47 was more slowly absorbed with peak levels occurring by 18 h and very slowly eliminated with an elimination half-life of 281 h.},
doi = {10.1016/j.cbpc.2006.09.006},
journal = {Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 145(1):86-95},
number = 1,
volume = 145,
place = {United States},
year = {Thu Feb 01 00:00:00 EST 2007},
month = {Thu Feb 01 00:00:00 EST 2007}
}