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Title: Protein Composition of the Vaccinia Virus Mature Virion

Abstract

The protein content of vaccinia virus mature virions, purified by rate zonal and isopycnic centrifugation and solubilized by SDS or a solution of urea and thiourea, was determined by the accurate mass and time tag technology which uses both tandem mass spectrometry and Fourier transform-ion cyclotron resonance mass spectrometry to detect tryptic peptides separated by high-resolution liquid chromatography. Eighty vaccinia virus-encoded proteins representing 37% of the 218 genes annotated in the complete genome sequence were detected in at least three analyses. Ten proteins accounted for approximately 80% of the mass, while the least abundant proteins made up 1% or less of the mass. Thirteen identified proteins were not previously reported as components of virions. On the other hand, 8 previously described virion proteins were not detected here, presumably due to technical reasons including small size and hydrophobicity. In addition to vaccinia virus-encoded proteins, 24 host proteins omitting isoforms were detected. The most abundant of these were cytoskeletal proteins, heat shock proteins, and proteins involved in translation.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
900917
Report Number(s):
PNNL-SA-51306
Journal ID: ISSN 0042-6822; VIRLAX; 10790; KP1704020
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology, 358(1):233-247; Journal Volume: 358; Journal Issue: 1
Country of Publication:
United States
Language:
English
Subject:
Environmental Molecular Sciences Laboratory

Citation Formats

Resch, Wolfgang, Hixson, Kim K., Moore, Ronald J., Lipton, Mary S., and Moss, Bernard. Protein Composition of the Vaccinia Virus Mature Virion. United States: N. p., 2007. Web. doi:10.1016/j.virol.2006.08.025.
Resch, Wolfgang, Hixson, Kim K., Moore, Ronald J., Lipton, Mary S., & Moss, Bernard. Protein Composition of the Vaccinia Virus Mature Virion. United States. doi:10.1016/j.virol.2006.08.025.
Resch, Wolfgang, Hixson, Kim K., Moore, Ronald J., Lipton, Mary S., and Moss, Bernard. Mon . "Protein Composition of the Vaccinia Virus Mature Virion". United States. doi:10.1016/j.virol.2006.08.025.
@article{osti_900917,
title = {Protein Composition of the Vaccinia Virus Mature Virion},
author = {Resch, Wolfgang and Hixson, Kim K. and Moore, Ronald J. and Lipton, Mary S. and Moss, Bernard},
abstractNote = {The protein content of vaccinia virus mature virions, purified by rate zonal and isopycnic centrifugation and solubilized by SDS or a solution of urea and thiourea, was determined by the accurate mass and time tag technology which uses both tandem mass spectrometry and Fourier transform-ion cyclotron resonance mass spectrometry to detect tryptic peptides separated by high-resolution liquid chromatography. Eighty vaccinia virus-encoded proteins representing 37% of the 218 genes annotated in the complete genome sequence were detected in at least three analyses. Ten proteins accounted for approximately 80% of the mass, while the least abundant proteins made up 1% or less of the mass. Thirteen identified proteins were not previously reported as components of virions. On the other hand, 8 previously described virion proteins were not detected here, presumably due to technical reasons including small size and hydrophobicity. In addition to vaccinia virus-encoded proteins, 24 host proteins omitting isoforms were detected. The most abundant of these were cytoskeletal proteins, heat shock proteins, and proteins involved in translation.},
doi = {10.1016/j.virol.2006.08.025},
journal = {Virology, 358(1):233-247},
number = 1,
volume = 358,
place = {United States},
year = {Mon Feb 05 00:00:00 EST 2007},
month = {Mon Feb 05 00:00:00 EST 2007}
}