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Title: Towards determining details of anaerobic growth coupled to ferric iron reduction by the acidophilic archaeon 'Ferroplasma acidarmanus' Fer1

Abstract

No abstract prepared.

Authors:
Publication Date:
Research Org.:
Savannah River Ecology Laboratory (SREL), Aiken, SC
Sponsoring Org.:
USDOE
OSTI Identifier:
898792
Report Number(s):
SREL-2999
Journal ID: ISSN 1431--0651; TRN: US200706%%256
DOE Contract Number:
DE-FC09-07SR22506
Resource Type:
Journal Article
Resource Relation:
Journal Name: Extremophiles; Journal Volume: 11; Journal Issue: 1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; IRON; REDUCTION; MICROORGANISMS; ANAEROBIC CONDITIONS; GROWTH; BIOCHEMICAL REACTION KINETICS

Citation Formats

Dopson, M., C. Baker-Austin and P. Bond. Towards determining details of anaerobic growth coupled to ferric iron reduction by the acidophilic archaeon 'Ferroplasma acidarmanus' Fer1. United States: N. p., 2007. Web. doi:10.1007/s00792-006-0029-y.
Dopson, M., C. Baker-Austin and P. Bond. Towards determining details of anaerobic growth coupled to ferric iron reduction by the acidophilic archaeon 'Ferroplasma acidarmanus' Fer1. United States. doi:10.1007/s00792-006-0029-y.
Dopson, M., C. Baker-Austin and P. Bond. Mon . "Towards determining details of anaerobic growth coupled to ferric iron reduction by the acidophilic archaeon 'Ferroplasma acidarmanus' Fer1". United States. doi:10.1007/s00792-006-0029-y.
@article{osti_898792,
title = {Towards determining details of anaerobic growth coupled to ferric iron reduction by the acidophilic archaeon 'Ferroplasma acidarmanus' Fer1},
author = {Dopson, M., C. Baker-Austin and P. Bond},
abstractNote = {No abstract prepared.},
doi = {10.1007/s00792-006-0029-y},
journal = {Extremophiles},
number = 1,
volume = 11,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2007},
month = {Mon Jan 01 00:00:00 EST 2007}
}
  • Studies were carried out to elucidate the nature and importance of Fe/sup 3 +/ reduction in anaerobic slurries of marine surface sediment. A constant accumulation of Fe/sup 2 +/ took place immediately after the endogenous NO/sub 3//sup -/ was depleted. Pasteurized controls showed no activity of Fe/sup 3 +/ reduction. Additions of 0.2 mM NO/sub 3//sup -/ and NO/sub 2//sup -/ to the active slurries arrested the Fe/sup 3 +/ reduction, and the process was resumed only after a depletion of the added compounds. Extended, initial aeration of the sediment did not affect the capacity for reduction of NO/sub 3//supmore » -/ and Fe/sup 3 +/, but the treatments with NO/sub 3//sup -/ increased the capacity for Fe/sup 3 -/ reduction. Addition of 20 mM MoO/sub 4//sup 2 -/ completely inhibited the SO/sub 4//sup 2 -/ reduction, but did not affect the reduction of Fe/sup 3 +/. The process of Fe/sup 3 +/ reduction was most likely associated with the activity of facultative anaerobic, NO/sub 3//sup -/- reducing bacteria. In surface sediment, the bulk of the Fe/sup 3 +/ reduction may be microbial, and the process may be important for mineralization in situ if the availability of NO/sub 3//sup -/ is low.« less
  • The potential for ferric iron reduction with fermentable substrates, fermentation products, and complex organic matter as electron donors was investigated with sediments from freshwater and brackish water sites in the Potomac River Estuary. In enrichments with glucose and hematite, iron reduction was a minor pathway for electron flow, and fermentation products accumulated. The substitution of amorphous ferric oxyhydroxide for hematite in glucose enrichments increased iron reduction 50-fold because the fermentation products could also be metabolized with concomitant iron reduction. Acetate, hydrogen, propionate, butyrate, ethanol, methanol, and trimethylamine stimulated the reduction of amorphous ferric oxyhydroxide in enrichments inoculated with sediments butmore » not in uninoculated or heat-killed controls. The addition of ferric iron inhibited methane production in sediments. The degree of inhibition of methane production by various forms of ferric iron was related to the effectiveness of these ferric compounds as electron acceptors for the metabolism of acetate. The addition of acetate or hydrogen relieved the inhibition of methane production by ferric iron. The decrease of electron equivalents proceeding to methane in sediments supplemented with amorphous ferric oxyhydroxides was compensated for by a corresponding increase of electron equivalents in ferrous iron. These results indicate that iron reduction can out compete methanogenic food chains for sediment organic matter. Thus, when amorphous ferric oxyhydroxides are available in anaerobic sediments, the transfer of electrons from organic matter to ferric iron can be a major pathway for organic matter decomposition.« less
  • Eight strains of Thiobacillus ferrooxidans and three strains of Thiobacillus were grown on ferrous iron (Fe{sup 2+}), elemental sulfur (S{sup 0}), or sulfide ore (Fe, Cu, Zn). The cells were studied for their aerobic Fe{sup 2+} and S{sup 0}-oxidizing activities (O{sub 2} consumption) and anaerobic S{sup 0}-oxidizing activity with ferric iron (Fe{sup 3+}) (Fe{sup 2+} formation). Results show that all the T. ferrooxidans strains studied have the ability to produce cells with Fe{sup 2+} and S{sup 0} oxidation and Fe{sup 3+} reduction activities, but their levels are influenced by growth substrates and strain differences.
  • Thiobacillus ferrooxidans, the bacterium most widely used in bioleaching or microbial desulfurization of coal, was grown in an electrolytic bioreactor containing a synthetic, ferrous sulfate medium. Passage of current through the medium reduced the bacterially generated ferric iron to the ferrous iron substrate. When used in conjunction with an inoculum that had been adapted to the electrolytic growth conditions, this technique increased the protein (cell) concentration by 3.7 times, increased the protein (cell) production rate by 6.5 times, increased the yield coefficient (cellular efficiency) by 8.0 times, and increased the ferrous iron oxidation rate by 1.5 times at 29/sup 0/C,more » compared with conventional cultivation techniques. A Monod-type equation with accepted values for the maximum specific growth rate could not account for the increased growth rate under electrolytic conditions.« less
  • No abstract prepared.