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Title: Chemical and biological differentiation of three human breast cancer cell types using time-of-flight secondary ion mass spectrometry (TOF-SIMS)

Abstract

We use Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS) to image and classify individual cells based on their characteristic mass spectra. Using statistical data reduction on the large data sets generated during TOF-SIMS analysis, similar biological materials can be differentiated based on a combination of small changes in protein expression, metabolic activity and cell structure. We apply this powerful technique to image and differentiate three carcinoma-derived human breast cancer cell lines (MCF-7, T47D and MDA-MB-231). In homogenized cells, we show the ability to differentiate the cell types as well as cellular compartments (cytosol, nuclear and membrane). These studies illustrate the capacity of TOF-SIMS to characterize individual cells by chemical composition, which could ultimately be applied to detect and identify single aberrant cells within a normal cell population. Ultimately, we anticipate characterizing rare chemical changes that may provide clues to single cell progression within carcinogenic and metastatic pathways.

Authors:
; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
898469
Report Number(s):
UCRL-JRNL-218036
TRN: US200706%%76
DOE Contract Number:
W-7405-ENG-48
Resource Type:
Journal Article
Resource Relation:
Journal Name: Analytical Chemistry, vol. 78, no. 11, June 1, 2006, pp. 3651-3658
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; BIOLOGICAL MATERIALS; CAPACITY; CHEMICAL COMPOSITION; COMPARTMENTS; MAMMARY GLANDS; MASS SPECTRA; MASS SPECTROSCOPY; NEOPLASMS; PROTEINS

Citation Formats

Kulp, K S, Berman, E F, Knize, M G, Shattuck, D L, Nelson, E J, Wu, L, Montgomery, J L, Felton, J S, and Wu, K J. Chemical and biological differentiation of three human breast cancer cell types using time-of-flight secondary ion mass spectrometry (TOF-SIMS). United States: N. p., 2006. Web.
Kulp, K S, Berman, E F, Knize, M G, Shattuck, D L, Nelson, E J, Wu, L, Montgomery, J L, Felton, J S, & Wu, K J. Chemical and biological differentiation of three human breast cancer cell types using time-of-flight secondary ion mass spectrometry (TOF-SIMS). United States.
Kulp, K S, Berman, E F, Knize, M G, Shattuck, D L, Nelson, E J, Wu, L, Montgomery, J L, Felton, J S, and Wu, K J. Mon . "Chemical and biological differentiation of three human breast cancer cell types using time-of-flight secondary ion mass spectrometry (TOF-SIMS)". United States. doi:. https://www.osti.gov/servlets/purl/898469.
@article{osti_898469,
title = {Chemical and biological differentiation of three human breast cancer cell types using time-of-flight secondary ion mass spectrometry (TOF-SIMS)},
author = {Kulp, K S and Berman, E F and Knize, M G and Shattuck, D L and Nelson, E J and Wu, L and Montgomery, J L and Felton, J S and Wu, K J},
abstractNote = {We use Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS) to image and classify individual cells based on their characteristic mass spectra. Using statistical data reduction on the large data sets generated during TOF-SIMS analysis, similar biological materials can be differentiated based on a combination of small changes in protein expression, metabolic activity and cell structure. We apply this powerful technique to image and differentiate three carcinoma-derived human breast cancer cell lines (MCF-7, T47D and MDA-MB-231). In homogenized cells, we show the ability to differentiate the cell types as well as cellular compartments (cytosol, nuclear and membrane). These studies illustrate the capacity of TOF-SIMS to characterize individual cells by chemical composition, which could ultimately be applied to detect and identify single aberrant cells within a normal cell population. Ultimately, we anticipate characterizing rare chemical changes that may provide clues to single cell progression within carcinogenic and metastatic pathways.},
doi = {},
journal = {Analytical Chemistry, vol. 78, no. 11, June 1, 2006, pp. 3651-3658},
number = ,
volume = ,
place = {United States},
year = {Mon Jan 09 00:00:00 EST 2006},
month = {Mon Jan 09 00:00:00 EST 2006}
}