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Title: Studies of multi-heme cytochromes from Geobacter sulfurreducens

Abstract

The Geobacteraceae family predominates in the reduction of uranium in subsurface environments. We are focusing on the model organism, Geobacter sulfurreducens; its genome contains a large number (>100) of cytochromes c that function in metal reduction pathways. Intensive functional genomics and physiological studies are in progress in Prof. Derek Lovley's laboratory, and the complete genome sequence of this organism has been determined by Methe et al. 2003. We are studying cytochromes from the c{sub 7} family that are required for the reduction of Fe(III). Previously, we expressed in E. coli (Londer et al., 2002) and determined the three-dimensional structure at 1.45 {angstrom} resolution (Pokkuluri et al., 2004a) of the three-heme cytochrome c{sub 7} (PpcA, coded by ORF01023) characterized by Lloyd et al., 2003. Further we identified in the G. sulfurreducens genome ORFs for several of its homologs (Pokkuluri et al., 2004a). Four of the ORFs are the same size as PpcA; three other ORFs are polymers of c7-type domains, two of which consist of four domains and one of nine domains, that contain 12 and 27 hemes respectively.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Biosciences Division, Argonne National Laboratory, Argonne, IL 60439
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
894934
Report Number(s):
CONF-ERSP2006-65
TRN: US200702%%463
DOE Contract Number:
W-31-109-Eng-38
Resource Type:
Conference
Resource Relation:
Conference: Annual Environmental Remediation Sciences Program PI Meeting, April 3-5, 2006, Warrenton, VA
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; CYTOCHROMES; FOCUSING; FUNCTIONALS; POLYMERS; RESOLUTION; URANIUM

Citation Formats

Pokkuluri, P. Raj, Londer, Yuri, Y., Orshonsky, Valerie, Orshonsky, Lisa, Duke, Norma, and Schiffer, Marianne. Studies of multi-heme cytochromes from Geobacter sulfurreducens. United States: N. p., 2006. Web.
Pokkuluri, P. Raj, Londer, Yuri, Y., Orshonsky, Valerie, Orshonsky, Lisa, Duke, Norma, & Schiffer, Marianne. Studies of multi-heme cytochromes from Geobacter sulfurreducens. United States.
Pokkuluri, P. Raj, Londer, Yuri, Y., Orshonsky, Valerie, Orshonsky, Lisa, Duke, Norma, and Schiffer, Marianne. Wed . "Studies of multi-heme cytochromes from Geobacter sulfurreducens". United States. doi:. https://www.osti.gov/servlets/purl/894934.
@article{osti_894934,
title = {Studies of multi-heme cytochromes from Geobacter sulfurreducens},
author = {Pokkuluri, P. Raj and Londer, Yuri, Y. and Orshonsky, Valerie and Orshonsky, Lisa and Duke, Norma and Schiffer, Marianne},
abstractNote = {The Geobacteraceae family predominates in the reduction of uranium in subsurface environments. We are focusing on the model organism, Geobacter sulfurreducens; its genome contains a large number (>100) of cytochromes c that function in metal reduction pathways. Intensive functional genomics and physiological studies are in progress in Prof. Derek Lovley's laboratory, and the complete genome sequence of this organism has been determined by Methe et al. 2003. We are studying cytochromes from the c{sub 7} family that are required for the reduction of Fe(III). Previously, we expressed in E. coli (Londer et al., 2002) and determined the three-dimensional structure at 1.45 {angstrom} resolution (Pokkuluri et al., 2004a) of the three-heme cytochrome c{sub 7} (PpcA, coded by ORF01023) characterized by Lloyd et al., 2003. Further we identified in the G. sulfurreducens genome ORFs for several of its homologs (Pokkuluri et al., 2004a). Four of the ORFs are the same size as PpcA; three other ORFs are polymers of c7-type domains, two of which consist of four domains and one of nine domains, that contain 12 and 27 hemes respectively.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Apr 05 00:00:00 EDT 2006},
month = {Wed Apr 05 00:00:00 EDT 2006}
}

Conference:
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