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Title: Cotton and Protein Interactions

Abstract

The adsorbent properties of important wound fluid proteins and cotton cellulose are reviewed. This review focuses on the adsorption of albumin to cotton-based wound dressings and some chemically modified derivatives targeted for chronic wounds. Adsorption of elastase in the presence of albumin was examined as a model to understand the interactive properties of these wound fluid components with cotton fibers. In the chronic non-healing wound, elastase appears to be over-expressed, and it digests tissue and growth factors, interfering with the normal healing process. Albumin is the most prevalent protein in wound fluid, and in highly to moderately exudative wounds, it may bind significantly to the fibers of wound dressings. Thus, the relative binding properties of both elastase and albumin to wound dressing fibers are of interest in the design of more effective wound dressings. The present work examines the binding of albumin to two different derivatives of cotton, and quantifies the elastase binding to the same derivatives following exposure of albumin to the fiber surface. An HPLC adsorption technique was employed coupled with a colorimetric enzyme assay to quantify the relative binding properties of albumin and elastase to cotton. The results of wound protein binding are discussed in relation tomore » the porosity and surface chemistry interactions of cotton and wound proteins. Studies are directed to understanding the implications of protein adsorption phenomena in terms of fiber-protein models that have implications for rationally designing dressings for chronic wounds.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
894851
Report Number(s):
PNWD-SA-6924
TRN: US200702%%432
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Book
Resource Relation:
Related Information: Modified Fibers with Medical and Specialty Applications, 49-65
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ADSORBENTS; ADSORPTION; ALBUMINS; CELLULOSE; CHEMISTRY; COTTON; DESIGN; ENZYMES; FIBERS; GROWTH FACTORS; HEALING; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; POROSITY; PROTEINS; WOUNDS

Citation Formats

Goheen, Steven C, Edwards, J V, Rayburn, Alfred R, Gaither, Kari A, and Castro, Nathan J. Cotton and Protein Interactions. United States: N. p., 2006. Web.
Goheen, Steven C, Edwards, J V, Rayburn, Alfred R, Gaither, Kari A, & Castro, Nathan J. Cotton and Protein Interactions. United States.
Goheen, Steven C, Edwards, J V, Rayburn, Alfred R, Gaither, Kari A, and Castro, Nathan J. 2006. "Cotton and Protein Interactions". United States.
@article{osti_894851,
title = {Cotton and Protein Interactions},
author = {Goheen, Steven C and Edwards, J V and Rayburn, Alfred R and Gaither, Kari A and Castro, Nathan J},
abstractNote = {The adsorbent properties of important wound fluid proteins and cotton cellulose are reviewed. This review focuses on the adsorption of albumin to cotton-based wound dressings and some chemically modified derivatives targeted for chronic wounds. Adsorption of elastase in the presence of albumin was examined as a model to understand the interactive properties of these wound fluid components with cotton fibers. In the chronic non-healing wound, elastase appears to be over-expressed, and it digests tissue and growth factors, interfering with the normal healing process. Albumin is the most prevalent protein in wound fluid, and in highly to moderately exudative wounds, it may bind significantly to the fibers of wound dressings. Thus, the relative binding properties of both elastase and albumin to wound dressing fibers are of interest in the design of more effective wound dressings. The present work examines the binding of albumin to two different derivatives of cotton, and quantifies the elastase binding to the same derivatives following exposure of albumin to the fiber surface. An HPLC adsorption technique was employed coupled with a colorimetric enzyme assay to quantify the relative binding properties of albumin and elastase to cotton. The results of wound protein binding are discussed in relation to the porosity and surface chemistry interactions of cotton and wound proteins. Studies are directed to understanding the implications of protein adsorption phenomena in terms of fiber-protein models that have implications for rationally designing dressings for chronic wounds.},
doi = {},
url = {https://www.osti.gov/biblio/894851}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jun 30 00:00:00 EDT 2006},
month = {Fri Jun 30 00:00:00 EDT 2006}
}

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