Dosage-Dependent Proteome Response of Shewanella oneidensis MR-1 to Chromate Insult
Conference
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OSTI ID:894627
Shewanella oneidensis MR-1 is a gram-negative, facultatively anaerobic bacterium originally isolated from a freshwater lake. S. oneidensis MR-1 has the ability to reduce toxic metal ions [e.g., Cr(VI) and U(VI)] found in industrial and governmental waste sites. Cells were grown and exposed to three different metal concentrations in order to probe the dosage response of S. oneidensis MR-1 to Cr(VI) in the form of chromate. Protein fractions were digested with trypsin and analyzed with a multidimensional HPLC-NanoESIMS/MS protocol. The goal of this work is to identify protein components of pathways/mechanisms responsible for both detoxification and reduction of chromate.
- Research Organization:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Purdue University, West Lafayette, IN
- Sponsoring Organization:
- USDOE Office of Science (SC)
- DOE Contract Number:
- AC05-00OR22725
- OSTI ID:
- 894627
- Report Number(s):
- CONF-ERSP2006-44; TRN: US200702%%136
- Resource Relation:
- Conference: Annual Environmental Remediation Sciences Program PI Meeting, April 3-5, 2006, Warrenton, VA
- Country of Publication:
- United States
- Language:
- English
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