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Title: Dosage-Dependent Proteome Response of Shewanella oneidensis MR-1 to Chromate Insult

Abstract

Shewanella oneidensis MR-1 is a gram-negative, facultatively anaerobic bacterium originally isolated from a freshwater lake. S. oneidensis MR-1 has the ability to reduce toxic metal ions [e.g., Cr(VI) and U(VI)] found in industrial and governmental waste sites. Cells were grown and exposed to three different metal concentrations in order to probe the dosage response of S. oneidensis MR-1 to Cr(VI) in the form of chromate. Protein fractions were digested with trypsin and analyzed with a multidimensional HPLC-NanoESIMS/MS protocol. The goal of this work is to identify protein components of pathways/mechanisms responsible for both detoxification and reduction of chromate.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN; Purdue University, West Lafayette, IN
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
894627
Report Number(s):
CONF-ERSP2006-44
TRN: US200702%%136
DOE Contract Number:
AC05-00OR22725
Resource Type:
Conference
Resource Relation:
Conference: Annual Environmental Remediation Sciences Program PI Meeting, April 3-5, 2006, Warrenton, VA
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; CHROMATES; DETOXIFICATION; PROBES; PROTEINS; TRYPSIN; WASTES

Citation Formats

Thompson, Melissa R., VerBerkmoes, Nathan C., Chourey, Karuna, Brown, Steven D., Hettich, Robert L., and Thompson, Dorothea K. Dosage-Dependent Proteome Response of Shewanella oneidensis MR-1 to Chromate Insult. United States: N. p., 2006. Web.
Thompson, Melissa R., VerBerkmoes, Nathan C., Chourey, Karuna, Brown, Steven D., Hettich, Robert L., & Thompson, Dorothea K. Dosage-Dependent Proteome Response of Shewanella oneidensis MR-1 to Chromate Insult. United States.
Thompson, Melissa R., VerBerkmoes, Nathan C., Chourey, Karuna, Brown, Steven D., Hettich, Robert L., and Thompson, Dorothea K. Wed . "Dosage-Dependent Proteome Response of Shewanella oneidensis MR-1 to Chromate Insult". United States. doi:. https://www.osti.gov/servlets/purl/894627.
@article{osti_894627,
title = {Dosage-Dependent Proteome Response of Shewanella oneidensis MR-1 to Chromate Insult},
author = {Thompson, Melissa R. and VerBerkmoes, Nathan C. and Chourey, Karuna and Brown, Steven D. and Hettich, Robert L. and Thompson, Dorothea K.},
abstractNote = {Shewanella oneidensis MR-1 is a gram-negative, facultatively anaerobic bacterium originally isolated from a freshwater lake. S. oneidensis MR-1 has the ability to reduce toxic metal ions [e.g., Cr(VI) and U(VI)] found in industrial and governmental waste sites. Cells were grown and exposed to three different metal concentrations in order to probe the dosage response of S. oneidensis MR-1 to Cr(VI) in the form of chromate. Protein fractions were digested with trypsin and analyzed with a multidimensional HPLC-NanoESIMS/MS protocol. The goal of this work is to identify protein components of pathways/mechanisms responsible for both detoxification and reduction of chromate.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Apr 05 00:00:00 EDT 2006},
month = {Wed Apr 05 00:00:00 EDT 2006}
}

Conference:
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