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Title: Natural Gene Transfer to Develop Resistance to Metal Toxicity in Microbial communities at the Oak Ridge FRC

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL), Upton, NY
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
894280
Report Number(s):
CONF-ERSP2006-5
R&D Project: EE-595-EEDA
Resource Type:
Conference
Resource Relation:
Conference: Annual Environmental Remediation Sciences Program PI Meeting, April 3-5, 2006, Warrenton, VA
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES

Citation Formats

Fitts, Jeffrey, Crosson, Garry, Van der Lelie, Daniel, Moreels, David, Taghavi, Safiyh, and Garafola, Craig. Natural Gene Transfer to Develop Resistance to Metal Toxicity in Microbial communities at the Oak Ridge FRC. United States: N. p., 2006. Web.
Fitts, Jeffrey, Crosson, Garry, Van der Lelie, Daniel, Moreels, David, Taghavi, Safiyh, & Garafola, Craig. Natural Gene Transfer to Develop Resistance to Metal Toxicity in Microbial communities at the Oak Ridge FRC. United States.
Fitts, Jeffrey, Crosson, Garry, Van der Lelie, Daniel, Moreels, David, Taghavi, Safiyh, and Garafola, Craig. Wed . "Natural Gene Transfer to Develop Resistance to Metal Toxicity in Microbial communities at the Oak Ridge FRC". United States. doi:. https://www.osti.gov/servlets/purl/894280.
@article{osti_894280,
title = {Natural Gene Transfer to Develop Resistance to Metal Toxicity in Microbial communities at the Oak Ridge FRC},
author = {Fitts, Jeffrey and Crosson, Garry and Van der Lelie, Daniel and Moreels, David and Taghavi, Safiyh and Garafola, Craig},
abstractNote = {},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Apr 05 00:00:00 EDT 2006},
month = {Wed Apr 05 00:00:00 EDT 2006}
}

Conference:
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  • The overall goal of this study is to evaluate structure-function relationships of sedimentary microbial communities likely to regulate U(VI) reduction and immobilization in the subsurface of Area 2 at the Field Research Center (FRC), Oak Ridge, TN. Microcosm experiments were conducted under near in situ conditions with FRC subsurface materials cocontaminated with high levels of U(VI) and nitrate. The activity, abundance, and community composition of microorganisms was determined in microcosm samples, stimulated with ethanol or glucose, and compared to those from sediment cores and unamended controls. Activity was assessed by monitoring terminal electron accepting processes (TEAPs; nitrate, sulfate, uranium, andmore » iron reduction) as well as electron donor utilization. Microbial functional groups, nitrate- and iron(III)-reducing bacteria, were enumerated during the nitrate- and metal-reduction phases of the incubation and in sediment core samples using a most probable number (MPN) serial dilution assay. U(VI) and Fe(III) were reduced concurrently in the glucose but not the ethanol treatments. In ethanol-amended microcosms, U(VI) was reduced during a 4-day lag phase between nitrate- and Fe(III)-reduction phases. Biostimulation resulted in 3 to 5 orders of magnitude higher counts of Fe(III)-reducing bacteria, whereas populations of nitrate-reducers were enhanced by 1 to 3 orders of magnitude. One to 2 orders of magnitude more Fe(III)-reducers were observed in ethanol- as compared to glucose-amended treatments in parallel with enhanced U(VI) removal in ethanol treatments. Cultivatable Fe(III)-reducing bacteria in the ethanol treatments were dominated by Geobacter sp. while those cultured on glucose were dominated by fermentative organisms, i.e., Tolumonas sp. Currently, carbon substrate utilization is being examined through HPLC analysis of microcosm porewaters. In addition, changes in the overall microbial community composition are being assessed using cultivation-independent techniques, including fluorescence in situ hybridization (FISH), terminal restriction fragment length polymorphism analysis (T-RFLP) and cloning/sequencing of structural and functional genes. Our results indicate that the microbially-catalyzed mechanism of U(VI) reduction is electron donor dependent and that more effective U(VI) removal is achieved in parallel with an enrichment of Geobacter sp. upon treatment with ethanol.« less
  • Microbial communities in water from Baltimore Harbor and from the mainstem of Chesapeake Bay were examined for sensitivity to mercuric chloride, monomethyl mercury, stannic chloride, and tributyltin chloride. Acute toxicity was determined by measuring the effects of (/sup 3/H)thymidine incorporation, (/sup 14/C)glutamate incorporation and respiration, and viability as compared with those of controls. Minimum inhibitory concentrations were low for all metals (monomethyl mercury, <0.05 ..mu..g liter/sup -1/; mercuric chloride, <1 ..mu..g liter/sup -1/; tributyltin chloride, <5 ..mu..g liter/sup -1/) except stannic chloride (5 mg liter/sup -1/). In some cases, mercuric chloride and monomethyl mercury were equally toxic at comparable concentrations.more » The Chesapeake Bay community appeared to be slightly more sensitive to metal stress than the Baltimore Harbor community, but this was not true for all treatments or assays. For culturable bacteria the opposite result was found. Thymidine incorporation and glutamate metabolism were much more sensitive indicators of metals for toxicity than was viability. To our knowledge, this is the first use of the thymindine incorporation methods ecotoxicology studies. We found it the easiest and fastest of the three methods; it is at least equal in sensitivity to metabolic measurements, and it likely measures the effects on the greater portion of the natural community.« less
  • Horizontal gene transfer as adaptive response to heavy metal stress in the presence of heavy metal stress was evaluated in oligotrophic subsurface soil laboratory scale microcosms. Increasing levels of cadmium (10, 100 and 1000 mM) were applied and an E. coli donor was used to deliver the target plasmids, pMOL187 and pMOL222, which contained the czc and ncc operons, and the helper plasmid RP4. Plasmid transfer was evaluated through monitoring of the heavy metal resistance and presence of the genes. The interactive, clearly revealed, effect of biological and chemical external factors on the extent of plasmid-DNA propagation in microbial communitiesmore » in contaminated soil environments was observed in this study. Additionally, P.putida LBJ 415 carrying a suicide construct was used to evaluate selective elimination of a plasmid donor.« less
  • Areas on the Oak Ridge Reservation (ORR) that contain rare plant or animal species or are special habitats are protected through National Environmental Research Park Natural Area (NA) or Reference Area (RA) designations. The US Department of Energy`s Oak Ridge National Environmental Research Park program is responsible for identifying species of vascular plants that are endangered, threatened, or rare and, as much as possible, for conserving those areas in which such species grow. This report includes a listing of Research Park NAs and RAs with general habitat descriptions and a computer-generated map with the areas identified. These are the locationsmore » of rare plant or animal species or special habitats that are known at this time. As the Reservation continues to be surveyed, it is expected that additional sites will be designated as Research Park NAs or RAs. This document is a component of a larger effort to identify environmentally sensitive areas on ORR. This report identifies the currently known locations of rare plant species, rare animal species, and special biological communities. Floodplains, wetlands (except those in RAs or NAs), and cultural resources are not included in this report.« less
  • No abstract prepared.