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Title: Hydrogen Production Using Hydrogenase-Containing Oxygenic Photosynthetic Organisms

Abstract

A reversible physiological process provides for the temporal separation of oxygen evolution and hydrogen production in a microorganism, which includes the steps of growing a culture of the microorganism in medium under illuminated conditions to accumulate an endogenous substrate, depleting from the medium a nutrient selected from the group consisting of sulfur, iron, and/or manganese, sealing the culture from atmospheric oxygen, incubating the culture in light whereby a rate of light-induced oxygen production is equal to or less than a rate of respiration, and collecting an evolved gas. The process is particularly useful to accomplish a sustained photobiological hydrogen gas production in cultures of microorganisms, such as Chlamydomonas reinhardtii.

Inventors:
; ; ; ; ;
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
894277
Patent Number(s):
U.S. Patent No. 6,989,252 B2
Application Number:
TRN: US200701%%293
Assignee:
NREL
DOE Contract Number:
AC36-99-GO10337
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
08 HYDROGEN; 59 BASIC BIOLOGICAL SCIENCES; CHLAMYDOMONAS; HYDROGEN; HYDROGEN PRODUCTION; IRON; MANGANESE; MICROORGANISMS; NUTRIENTS; OXYGEN; PRODUCTION; RESPIRATION; SULFUR; HYDROGENASE; OXYGENIC PHOTOSYNTHETIC ORGANISMS; ENDOGENOUS SUBSTRATE; PHOTOBIOLOGICAL HYDROGEN GAS PRODUCTION; Basic Sciences; Photoconversion; Hydrogen

Citation Formats

Melis, A., Zhang, L., Benemann, J. R., Forestier, M., Ghirardi, M., and Seibert, M. Hydrogen Production Using Hydrogenase-Containing Oxygenic Photosynthetic Organisms. United States: N. p., 2006. Web.
Melis, A., Zhang, L., Benemann, J. R., Forestier, M., Ghirardi, M., & Seibert, M. Hydrogen Production Using Hydrogenase-Containing Oxygenic Photosynthetic Organisms. United States.
Melis, A., Zhang, L., Benemann, J. R., Forestier, M., Ghirardi, M., and Seibert, M. Tue . "Hydrogen Production Using Hydrogenase-Containing Oxygenic Photosynthetic Organisms". United States. doi:.
@article{osti_894277,
title = {Hydrogen Production Using Hydrogenase-Containing Oxygenic Photosynthetic Organisms},
author = {Melis, A. and Zhang, L. and Benemann, J. R. and Forestier, M. and Ghirardi, M. and Seibert, M.},
abstractNote = {A reversible physiological process provides for the temporal separation of oxygen evolution and hydrogen production in a microorganism, which includes the steps of growing a culture of the microorganism in medium under illuminated conditions to accumulate an endogenous substrate, depleting from the medium a nutrient selected from the group consisting of sulfur, iron, and/or manganese, sealing the culture from atmospheric oxygen, incubating the culture in light whereby a rate of light-induced oxygen production is equal to or less than a rate of respiration, and collecting an evolved gas. The process is particularly useful to accomplish a sustained photobiological hydrogen gas production in cultures of microorganisms, such as Chlamydomonas reinhardtii.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 24 00:00:00 EST 2006},
month = {Tue Jan 24 00:00:00 EST 2006}
}