Hydrogen Production Using Hydrogenase-Containing Oxygenic Photosynthetic Organisms
A reversible physiological process provides for the temporal separation of oxygen evolution and hydrogen production in a microorganism, which includes the steps of growing a culture of the microorganism in medium under illuminated conditions to accumulate an endogenous substrate, depleting from the medium a nutrient selected from the group consisting of sulfur, iron, and/or manganese, sealing the culture from atmospheric oxygen, incubating the culture in light whereby a rate of light-induced oxygen production is equal to or less than a rate of respiration, and collecting an evolved gas. The process is particularly useful to accomplish a sustained photobiological hydrogen gas production in cultures of microorganisms, such as Chlamydomonas reinhardtii.
- Research Organization:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC36-99-GO10337
- Assignee:
- NREL
- Patent Number(s):
- U.S. Patent No. 6,989,252 B2
- Application Number:
- TRN: US200701%%293
- OSTI ID:
- 894277
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CHLAMYDOMONAS
HYDROGEN
HYDROGEN PRODUCTION
IRON
MANGANESE
MICROORGANISMS
NUTRIENTS
OXYGEN
PRODUCTION
RESPIRATION
SULFUR
HYDROGENASE
OXYGENIC PHOTOSYNTHETIC ORGANISMS
ENDOGENOUS SUBSTRATE
PHOTOBIOLOGICAL HYDROGEN GAS PRODUCTION
Basic Sciences
Photoconversion
Hydrogen