Advances and Challenges in Liquid Chromatography-Mass Spectrometry-Based Proteomics Profiling for Clinical Applications
The advances in proteomic technologies provide tremendous opportunities for applying these technologies in biomarker-related clinical applications; however, the unique characteristics of human biofluids such as high dynamic range in protein abundances and extreme complexity of human proteomes present tremendous challenges for current analytical technologies. In this review, we focus on summarizing the recent advances in LC-MS based proteomic profiling and its applications in clinical proteomics as well as the major challenges for implementing these technologies for more effective biomarker discovery. Over the last few years, tremendous efforts have been directed towards the development of more effective approaches for characterizing the human plasma/serum and other biofluid proteomes. The developments in immunodepletion and various fractionation approaches in combination with much improved LC-MS platforms have enabled the profiling of the plasma proteome with much greater dynamic range of coverage, allowing many proteins at low ng/mL levels being confidently identified. Despite the significant advances and efforts, the dynamic range of measurements or extent of proteome coverage, the confidence of peptide/protein identification, the accuracy of quantitation, the throughput of analysis, and the robustness of the present instrumentation are still among the major challenges for implementation of a proteomic profiling platform suitable for efficient clinical applications.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 893658
- Report Number(s):
- PNWD-SA-7399; 12500a; 12695; 17798; 17799; 16725; TRN: US200702%%622
- Journal Information:
- Molecular & Cellular Proteomics. MCP, 5(10):1727-1744, Journal Name: Molecular & Cellular Proteomics. MCP, 5(10):1727-1744
- Country of Publication:
- United States
- Language:
- English
Similar Records
The clinical impact of recent advances in LC-MS for cancer biomarker discovery and verification
The Use of a Quantitative Cysteinyl-peptide Enrichment Technology for High-Throughput Quantitative Proteomics