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Title: Shewanella loihica sp. nov., isolated from iron-rich microbial mats in the Pacific Ocean

Abstract

A novel marine bacterial strain, PV-4T, isolated from a microbial mat located at a hydrothermal vent of Loihi Seamount in the Pacific Ocean, has been characterized. This micro-organism is orange in color, Gram-negative, polarly flagellated, facultatively anaerobic and psychrotolerant (temperature range, 0-42 C). No growth was observed with nitrate, nitrite, DMSO or thiosulfate as the electron acceptor and lactate as the electron donor. The major fatty acid detected in strain PV-4T was iso-C15 : 0. Strain PV-4T had ubiquinones consisting mainly of Q-7 and Q-8, and possessed menaquinone MK-7. The DNA G+C content of the strain was 53.8 mol% and the genome size was about 4.5 Mbp. Phylogenetic analyses based on 16S rRNA gene sequences placed PV-4T within the genus Shewanella. PV-4T exhibited 16S rRNA gene sequence similarity levels of 99.6 and 97.5 %, respectively, with respect to the type strains of Shewanella aquimarina and Shewanella marisflavi. DNA from strain PV-4T showed low mean levels of relatedness to the DNAs of S. aquimarina (50.5%) and S. marisflavi (8.5%). On the basis of phylogenetic and phenotypic characteristics, the bacterium was classified in the genus Shewanella within a distinct novel species, for which the name Shewanella loihica sp. nov. is proposed. Themore » type strain is PV-4T (=ATCC BAA-1088T=DSM 17748T).« less

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
892898
Report Number(s):
PNNL-SA-51439
3204; TRN: US200623%%617
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
International Journal of Systematic and Evolutionary Microbiology, 56(8):1911-1916
Additional Journal Information:
Journal Name: International Journal of Systematic and Evolutionary Microbiology, 56(8):1911-1916
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BINDING ENERGY; CARBOXYLIC ACIDS; COLOR; DNA; ELECTRONS; GENES; LACTATES; ORANGES; PACIFIC OCEAN; STRAINS; THIOSULFATES; VALENCE; Environmental Molecular Sciences Laboratory

Citation Formats

Gao, Haichun, Obraztova, Anna, Stewart, Nathan, Popa, Radu, Fredrickson, Jim K, Tiedje, James M, Nealson, Kenneth, and Zhou, Jizhong. Shewanella loihica sp. nov., isolated from iron-rich microbial mats in the Pacific Ocean. United States: N. p., 2006. Web. doi:10.1099/ijs.0.64354-0.
Gao, Haichun, Obraztova, Anna, Stewart, Nathan, Popa, Radu, Fredrickson, Jim K, Tiedje, James M, Nealson, Kenneth, & Zhou, Jizhong. Shewanella loihica sp. nov., isolated from iron-rich microbial mats in the Pacific Ocean. United States. doi:10.1099/ijs.0.64354-0.
Gao, Haichun, Obraztova, Anna, Stewart, Nathan, Popa, Radu, Fredrickson, Jim K, Tiedje, James M, Nealson, Kenneth, and Zhou, Jizhong. Mon . "Shewanella loihica sp. nov., isolated from iron-rich microbial mats in the Pacific Ocean". United States. doi:10.1099/ijs.0.64354-0.
@article{osti_892898,
title = {Shewanella loihica sp. nov., isolated from iron-rich microbial mats in the Pacific Ocean},
author = {Gao, Haichun and Obraztova, Anna and Stewart, Nathan and Popa, Radu and Fredrickson, Jim K and Tiedje, James M and Nealson, Kenneth and Zhou, Jizhong},
abstractNote = {A novel marine bacterial strain, PV-4T, isolated from a microbial mat located at a hydrothermal vent of Loihi Seamount in the Pacific Ocean, has been characterized. This micro-organism is orange in color, Gram-negative, polarly flagellated, facultatively anaerobic and psychrotolerant (temperature range, 0-42 C). No growth was observed with nitrate, nitrite, DMSO or thiosulfate as the electron acceptor and lactate as the electron donor. The major fatty acid detected in strain PV-4T was iso-C15 : 0. Strain PV-4T had ubiquinones consisting mainly of Q-7 and Q-8, and possessed menaquinone MK-7. The DNA G+C content of the strain was 53.8 mol% and the genome size was about 4.5 Mbp. Phylogenetic analyses based on 16S rRNA gene sequences placed PV-4T within the genus Shewanella. PV-4T exhibited 16S rRNA gene sequence similarity levels of 99.6 and 97.5 %, respectively, with respect to the type strains of Shewanella aquimarina and Shewanella marisflavi. DNA from strain PV-4T showed low mean levels of relatedness to the DNAs of S. aquimarina (50.5%) and S. marisflavi (8.5%). On the basis of phylogenetic and phenotypic characteristics, the bacterium was classified in the genus Shewanella within a distinct novel species, for which the name Shewanella loihica sp. nov. is proposed. The type strain is PV-4T (=ATCC BAA-1088T=DSM 17748T).},
doi = {10.1099/ijs.0.64354-0},
journal = {International Journal of Systematic and Evolutionary Microbiology, 56(8):1911-1916},
number = ,
volume = ,
place = {United States},
year = {2006},
month = {8}
}