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Title: Detection of biological particles in ambient air using Bio-Aerosol Mass Spectrometry

Abstract

The Bio-Aerosol Mass Spectrometry (BAMS) system is an instrument used for the real time detection and identification of biological aerosols. Particles are drawn from the atmosphere directly into vacuum and tracked as they scatter light from several continuous wave lasers. After tracking, the fluorescence of individual particles is excited by a pulsed 266nm or 355nm laser. Molecules from those particles with appropriate fluorescence properties are subsequently desorbed and ionized using a pulsed 266nm laser. Resulting ions are analyzed in a dual polarity mass spectrometer. During two field deployments at the San Francisco International Airport, millions of ambient particles were analyzed and a small but significant fraction were found to have fluorescent properties similar to Bacillus spores and vegetative cells. Further separation of non-biological background particles from potential biological particles was accomplished using laser desorption/ionization mass spectrometry. This has been shown to enable some level of species differentiation in specific cases, but the creation and observation of higher mass ions is needed to enable a higher level of specificity across more species. A soft ionization technique, matrix-assisted laser desorption/ionization (MALDI) is being investigated for this purpose. MALDI is particularly well suited for mass analysis of biomolecules since it allows for themore » generation of molecular ions from large mass compounds that would fragment under normal irradiation. Some of the initial results from a modified BAMS system utilizing this technique are described.« less

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
891060
Report Number(s):
UCRL-CONF-220443
TRN: US0604906
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Conference
Resource Relation:
Conference: Presented at: SPIE Defense&Security Symposium, Kissimmee, FL, United States, Apr 17 - Apr 21, 2006
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; 74 ATOMIC AND MOLECULAR PHYSICS; AEROSOLS; AIR; BACILLUS; DETECTION; FLUORESCENCE; IONIZATION; IRRADIATION; LASERS; MASS SPECTROMETERS; MASS SPECTROSCOPY; MOLECULAR IONS; SECURITY; SPECIFICITY; SPORES

Citation Formats

McJimpsey, E L, Steele, P T, Coffee, K R, Fergenson, D P, Riot, V J, Woods, B W, Gard, E E, Frank, M, Tobias, H J, and Lebrilla, C. Detection of biological particles in ambient air using Bio-Aerosol Mass Spectrometry. United States: N. p., 2006. Web.
McJimpsey, E L, Steele, P T, Coffee, K R, Fergenson, D P, Riot, V J, Woods, B W, Gard, E E, Frank, M, Tobias, H J, & Lebrilla, C. Detection of biological particles in ambient air using Bio-Aerosol Mass Spectrometry. United States.
McJimpsey, E L, Steele, P T, Coffee, K R, Fergenson, D P, Riot, V J, Woods, B W, Gard, E E, Frank, M, Tobias, H J, and Lebrilla, C. Thu . "Detection of biological particles in ambient air using Bio-Aerosol Mass Spectrometry". United States. doi:. https://www.osti.gov/servlets/purl/891060.
@article{osti_891060,
title = {Detection of biological particles in ambient air using Bio-Aerosol Mass Spectrometry},
author = {McJimpsey, E L and Steele, P T and Coffee, K R and Fergenson, D P and Riot, V J and Woods, B W and Gard, E E and Frank, M and Tobias, H J and Lebrilla, C},
abstractNote = {The Bio-Aerosol Mass Spectrometry (BAMS) system is an instrument used for the real time detection and identification of biological aerosols. Particles are drawn from the atmosphere directly into vacuum and tracked as they scatter light from several continuous wave lasers. After tracking, the fluorescence of individual particles is excited by a pulsed 266nm or 355nm laser. Molecules from those particles with appropriate fluorescence properties are subsequently desorbed and ionized using a pulsed 266nm laser. Resulting ions are analyzed in a dual polarity mass spectrometer. During two field deployments at the San Francisco International Airport, millions of ambient particles were analyzed and a small but significant fraction were found to have fluorescent properties similar to Bacillus spores and vegetative cells. Further separation of non-biological background particles from potential biological particles was accomplished using laser desorption/ionization mass spectrometry. This has been shown to enable some level of species differentiation in specific cases, but the creation and observation of higher mass ions is needed to enable a higher level of specificity across more species. A soft ionization technique, matrix-assisted laser desorption/ionization (MALDI) is being investigated for this purpose. MALDI is particularly well suited for mass analysis of biomolecules since it allows for the generation of molecular ions from large mass compounds that would fragment under normal irradiation. Some of the initial results from a modified BAMS system utilizing this technique are described.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Mar 16 00:00:00 EST 2006},
month = {Thu Mar 16 00:00:00 EST 2006}
}

Conference:
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