skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Unraveling the Structure-Function Relationships of Microbial Systems by High-Resolution in vitro Atomic Force Microscopy

Abstract

The elucidation of microbial surface architecture is critical to determining mechanisms of pathogenesis, immune response, physicochemical properties and environmental resistance. We have utilized in vitro AFM for studies of structure, assembly, function and environmental dynamics of several microbial systems including bacteria and bacterial spores. We have demonstrated, using various species of bacterial spores strikingly different species-dependent crystalline structures of the spore coat appear to be a consequence of crystallization mechanisms that regulate the assembly of the spore coat. Furthermore, we revealed molecular-scale transformations of the spore coat and cell outgrowth during the germination process. We will present data on the direct visualization of stress-induced environmental response of metal-resistant Arthrobacter oxydans bacteria to Cr (VI) exposure. These studies demonstrate that in vitro AFM can probe microbial surface architecture, environmental dynamics and the life cycle of pathogens at near-molecular resolution under physiological conditions.

Authors:
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
891055
Report Number(s):
UCRL-CONF-220631
TRN: US200621%%75
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Conference
Resource Relation:
Conference: Presented at: Seeing at NanoScale IV, Philadelphia, PA, United States, Jul 17 - Jul 20, 2006
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ARCHITECTURE; ATOMIC FORCE MICROSCOPY; BACTERIA; BACTERIAL SPORES; CRYSTALLIZATION; GERMINATION; IN VITRO; LIFE CYCLE; PATHOGENESIS; PATHOGENS; PROBES; RESOLUTION; SPORES; TRANSFORMATIONS

Citation Formats

Malkin, A. Unraveling the Structure-Function Relationships of Microbial Systems by High-Resolution in vitro Atomic Force Microscopy. United States: N. p., 2006. Web.
Malkin, A. Unraveling the Structure-Function Relationships of Microbial Systems by High-Resolution in vitro Atomic Force Microscopy. United States.
Malkin, A. Thu . "Unraveling the Structure-Function Relationships of Microbial Systems by High-Resolution in vitro Atomic Force Microscopy". United States. doi:. https://www.osti.gov/servlets/purl/891055.
@article{osti_891055,
title = {Unraveling the Structure-Function Relationships of Microbial Systems by High-Resolution in vitro Atomic Force Microscopy},
author = {Malkin, A},
abstractNote = {The elucidation of microbial surface architecture is critical to determining mechanisms of pathogenesis, immune response, physicochemical properties and environmental resistance. We have utilized in vitro AFM for studies of structure, assembly, function and environmental dynamics of several microbial systems including bacteria and bacterial spores. We have demonstrated, using various species of bacterial spores strikingly different species-dependent crystalline structures of the spore coat appear to be a consequence of crystallization mechanisms that regulate the assembly of the spore coat. Furthermore, we revealed molecular-scale transformations of the spore coat and cell outgrowth during the germination process. We will present data on the direct visualization of stress-induced environmental response of metal-resistant Arthrobacter oxydans bacteria to Cr (VI) exposure. These studies demonstrate that in vitro AFM can probe microbial surface architecture, environmental dynamics and the life cycle of pathogens at near-molecular resolution under physiological conditions.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Apr 06 00:00:00 EDT 2006},
month = {Thu Apr 06 00:00:00 EDT 2006}
}

Conference:
Other availability
Please see Document Availability for additional information on obtaining the full-text document. Library patrons may search WorldCat to identify libraries that hold this conference proceeding.

Save / Share: