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Title: Oxidative Stress and Heat-Shock Responses in Desulfovibrio vulgaris by Genome-Wide Transcriptomic Analysis

Abstract

Abstract Sulfate-reducing bacteria, like Desulfovibrio vulgaris have developed a set of reactions allowing them to survive in environments. To obtain further knowledge of the protecting mechanisms employed in D. vulgaris against the oxidative stress and heat shock, we performed a genome-wide transcriptomic analysis to determine the cellular responses to both stimuli. The results showed that 130 genes were responsive to oxidative stress, while 427 genes responsive to heat-shock, respectively. Functional analyses suggested that the genes regulated were involved in a variety of cellular functions. Metabolic analysis showed that amino acid biosynthetic pathways were induced by both oxidative stress and heat shock treatments, while fatty acid metabolism, purine and cofactor biosynthesis were induced by heat shock only. Rubrerythrin gene (rbR) were upregulated by the oxidative stress, suggesting its important role in the oxidative resistance, whereas the expression of rubredoxin oxidoreductase (rbO), superoxide ismutase (sodB) and catalase (katA) genes were not subjected to regulation by oxidative stress in D. vulgaris. In addition, the results showed that thioredoxin reductase (trxB) was responsive to oxidative stress, suggesting the thiol-specific redox system might be involved in oxidative protection in D. vulgaris. Comparison of cellular responses to oxidative stress and heat-shock allowed the identification of 66more » genes that showed a similar drastic response to both environmental stimuli, implying that they might be part of the general stress response (GSR) network in D. vulgaris, which was further supported by the finding of a conserved motif upstream these common-responsive genes.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
889039
Report Number(s):
PNNL-SA-45443
Journal ID: ISSN 0003-6072; ALJMAO; TRN: US200619%%336
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Antonie Van Leeuwenhoek, 90:41-55
Additional Journal Information:
Journal Volume: 90; Journal ID: ISSN 0003-6072
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; AMINO ACIDS; BIOSYNTHESIS; CARBOXYLIC ACIDS; CATALASE; DESULFOVIBRIO; FUNCTIONALS; GENES; METABOLISM; OXIDOREDUCTASES; PURINES; REGULATIONS; RUBREDOXIN; STIMULI; SULFATE-REDUCING BACTERIA

Citation Formats

Zhang, Weiwen, Culley, David E, Hogan, Mike, Vitiritti, Luigi, and Brockman, Fred J. Oxidative Stress and Heat-Shock Responses in Desulfovibrio vulgaris by Genome-Wide Transcriptomic Analysis. United States: N. p., 2006. Web. doi:10.1007/s10482-006-9059-9.
Zhang, Weiwen, Culley, David E, Hogan, Mike, Vitiritti, Luigi, & Brockman, Fred J. Oxidative Stress and Heat-Shock Responses in Desulfovibrio vulgaris by Genome-Wide Transcriptomic Analysis. United States. doi:10.1007/s10482-006-9059-9.
Zhang, Weiwen, Culley, David E, Hogan, Mike, Vitiritti, Luigi, and Brockman, Fred J. Tue . "Oxidative Stress and Heat-Shock Responses in Desulfovibrio vulgaris by Genome-Wide Transcriptomic Analysis". United States. doi:10.1007/s10482-006-9059-9.
@article{osti_889039,
title = {Oxidative Stress and Heat-Shock Responses in Desulfovibrio vulgaris by Genome-Wide Transcriptomic Analysis},
author = {Zhang, Weiwen and Culley, David E and Hogan, Mike and Vitiritti, Luigi and Brockman, Fred J},
abstractNote = {Abstract Sulfate-reducing bacteria, like Desulfovibrio vulgaris have developed a set of reactions allowing them to survive in environments. To obtain further knowledge of the protecting mechanisms employed in D. vulgaris against the oxidative stress and heat shock, we performed a genome-wide transcriptomic analysis to determine the cellular responses to both stimuli. The results showed that 130 genes were responsive to oxidative stress, while 427 genes responsive to heat-shock, respectively. Functional analyses suggested that the genes regulated were involved in a variety of cellular functions. Metabolic analysis showed that amino acid biosynthetic pathways were induced by both oxidative stress and heat shock treatments, while fatty acid metabolism, purine and cofactor biosynthesis were induced by heat shock only. Rubrerythrin gene (rbR) were upregulated by the oxidative stress, suggesting its important role in the oxidative resistance, whereas the expression of rubredoxin oxidoreductase (rbO), superoxide ismutase (sodB) and catalase (katA) genes were not subjected to regulation by oxidative stress in D. vulgaris. In addition, the results showed that thioredoxin reductase (trxB) was responsive to oxidative stress, suggesting the thiol-specific redox system might be involved in oxidative protection in D. vulgaris. Comparison of cellular responses to oxidative stress and heat-shock allowed the identification of 66 genes that showed a similar drastic response to both environmental stimuli, implying that they might be part of the general stress response (GSR) network in D. vulgaris, which was further supported by the finding of a conserved motif upstream these common-responsive genes.},
doi = {10.1007/s10482-006-9059-9},
journal = {Antonie Van Leeuwenhoek, 90:41-55},
issn = {0003-6072},
number = ,
volume = 90,
place = {United States},
year = {2006},
month = {5}
}