skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Gene discovery in the Acanthamoeba castellanii genome

Abstract

Acanthamoeba castellanii is a free-living amoeba found in soil, freshwater, and marine environments and an important predator of bacteria. Acanthamoeba castellanii is also an opportunistic pathogen of clinical interest, responsible for several distinct diseases in humans. In order to provide a genomic platform for the study of this ubiquitous and important protist, we generated a sequence survey of approximately 0.5 x coverage of the genome. The data predict that A. castellanii exhibits a greater biosynthetic capacity than the free-living Dictyostelium discoideum and the parasite Entamoeba histolytica, providing an explanation for the ability of A. castellanii to inhabit adversity of environments. Alginate lyase may provide access to bacteria within biofilms by breaking down the biofilm matrix, and polyhydroxybutyrate depolymerase may facilitate utilization of the bacterial storage compound polyhydroxybutyrate as a food source. Enzymes for the synthesis and breakdown of cellulose were identified, and they likely participate in encystation and excystation as in D. discoideum. Trehalose-6-phosphate synthase is present, suggesting that trehalose plays a role in stress adaptation. Detection and response to a number of stress conditions is likely accomplished with a large set of signal transduction histidine kinases and a set of putative receptorserine/threonine kinases similar to those found in E.more » histolytica. Serine, cysteine and metalloproteases were identified, some of which are likely involved in pathogenicity.« less

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Ernest Orlando Lawrence Berkeley NationalLaboratory, Berkeley, CA (US)
Sponsoring Org.:
USDOE Director. Office of Science. Office of AdvancedScientific Computing Research. Office of Biological and EnvironmentalResearch; National Institutes of Health Grant A148082; Genome Canada,Atlatic Canada Opportunities Agency. Atlantic Innovation Fund. GenomeAtlantic
OSTI Identifier:
888974
Report Number(s):
LBNL-60431
R&D Project: 626869; BnR: KP1103010; TRN: US200619%%295
DOE Contract Number:  
DE-AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
Protist
Additional Journal Information:
Journal Volume: 156; Journal Issue: 2; Related Information: Journal Publication Date: August 2005
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ALGINATES; AMOEBA; BACTERIA; BREAKDOWN; CELLULOSE; CYSTEINE; DETECTION; DISEASES; ENZYMES; FOOD; GENES; HISTIDINE; LYASES; PARASITES; PATHOGENS; PHOSPHOTRANSFERASES; SERINE; SYNTHESIS; acanthamoeba metabolism genome

Citation Formats

Anderson, Iain J., Watkins, Russell F., Samuelson, John, Spencer,David F., Majoros, William H., Gray, Michael W., and Loftus, Brendan J. Gene discovery in the Acanthamoeba castellanii genome. United States: N. p., 2005. Web. doi:10.1016/j.protis.2005.04.001.
Anderson, Iain J., Watkins, Russell F., Samuelson, John, Spencer,David F., Majoros, William H., Gray, Michael W., & Loftus, Brendan J. Gene discovery in the Acanthamoeba castellanii genome. United States. doi:10.1016/j.protis.2005.04.001.
Anderson, Iain J., Watkins, Russell F., Samuelson, John, Spencer,David F., Majoros, William H., Gray, Michael W., and Loftus, Brendan J. Mon . "Gene discovery in the Acanthamoeba castellanii genome". United States. doi:10.1016/j.protis.2005.04.001.
@article{osti_888974,
title = {Gene discovery in the Acanthamoeba castellanii genome},
author = {Anderson, Iain J. and Watkins, Russell F. and Samuelson, John and Spencer,David F. and Majoros, William H. and Gray, Michael W. and Loftus, Brendan J.},
abstractNote = {Acanthamoeba castellanii is a free-living amoeba found in soil, freshwater, and marine environments and an important predator of bacteria. Acanthamoeba castellanii is also an opportunistic pathogen of clinical interest, responsible for several distinct diseases in humans. In order to provide a genomic platform for the study of this ubiquitous and important protist, we generated a sequence survey of approximately 0.5 x coverage of the genome. The data predict that A. castellanii exhibits a greater biosynthetic capacity than the free-living Dictyostelium discoideum and the parasite Entamoeba histolytica, providing an explanation for the ability of A. castellanii to inhabit adversity of environments. Alginate lyase may provide access to bacteria within biofilms by breaking down the biofilm matrix, and polyhydroxybutyrate depolymerase may facilitate utilization of the bacterial storage compound polyhydroxybutyrate as a food source. Enzymes for the synthesis and breakdown of cellulose were identified, and they likely participate in encystation and excystation as in D. discoideum. Trehalose-6-phosphate synthase is present, suggesting that trehalose plays a role in stress adaptation. Detection and response to a number of stress conditions is likely accomplished with a large set of signal transduction histidine kinases and a set of putative receptorserine/threonine kinases similar to those found in E. histolytica. Serine, cysteine and metalloproteases were identified, some of which are likely involved in pathogenicity.},
doi = {10.1016/j.protis.2005.04.001},
journal = {Protist},
number = 2,
volume = 156,
place = {United States},
year = {2005},
month = {8}
}