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Title: The Pathogenomic Sequence Analysis of B. cereus and B. Thuringiensis isolates closely related to Bacillus anthracis

Abstract

The sequencing and analysis of two close relatives of Bacillus anthracis are reported. AFLP analysis of over 300 isolates of B. cereus, B. thuringiensis and B. anthracis identified two isolates as being very closely related to B. anthracis. One, a B. cereus, BcE33L, was isolated from a zebra carcass in Nambia; the second, a B. thuringiensis, 97-27, was isolated from a necrotic human wound. The B. cereus appears to be the closest anthracis relative sequenced to date. A core genome of over 3,900 genes was compiled for the Bacillus cereus group, including B anthracis. Comparative analysis of these two genomes with other members of the B. cereus group provides insight into the evolutionary relationships among these organisms. Evidence is presented that differential regulation modulates virulence, rather than simple acquisition of virulence factors. These genome sequences provide insight into the molecular mechanisms contributing to the host range and virulence of this group of organisms.

Authors:
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Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
885390
Report Number(s):
UCRL-JRNL-216210
Journal ID: ISSN 0021-9193; JOBAAY; TRN: US200616%%598
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Journal Article
Journal Name:
Journal of Bacteriology
Additional Journal Information:
Journal Volume: 180; Journal Issue: 9; Journal ID: ISSN 0021-9193
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BACILLUS; BACILLUS CEREUS; GENES; REGULATIONS; STRUCTURAL CHEMICAL ANALYSIS; VIRULENCE

Citation Formats

Han, C S, Xie, G, Challacombe, J F, Altherr, M R, Bhotika, S S, Bruce, D, Campbell, C S, Campbell, M L, Chen, J, Chertkov, O, Cleland, C, Dimitrijevic-Bussod, M, Doggett, N A, Fawcett, J J, Glavina, T, Goodwin, L A, Hill, K K, Hitchcock, P, Jackson, P J, Keim, P, Kewalramani, A R, Longmire, J, Lucas, S, Malfatti, S, McMurry, K, Meincke, L J, Misra, M, Moseman, B L, Mundt, M, Munk, A C, Okinaka, R T, Parson-Quintana, B, Reilly, L P, Richardson, P, Robinson, D L, Rubin, E, Saunders, E, Tapia, R, Tesmer, J G, Thayer, N, Thompson, L S, Tice, H, Ticknor, L O, Wills, P L, Gilna, P, and Brettin, T S. The Pathogenomic Sequence Analysis of B. cereus and B. Thuringiensis isolates closely related to Bacillus anthracis. United States: N. p., 2005. Web.
Han, C S, Xie, G, Challacombe, J F, Altherr, M R, Bhotika, S S, Bruce, D, Campbell, C S, Campbell, M L, Chen, J, Chertkov, O, Cleland, C, Dimitrijevic-Bussod, M, Doggett, N A, Fawcett, J J, Glavina, T, Goodwin, L A, Hill, K K, Hitchcock, P, Jackson, P J, Keim, P, Kewalramani, A R, Longmire, J, Lucas, S, Malfatti, S, McMurry, K, Meincke, L J, Misra, M, Moseman, B L, Mundt, M, Munk, A C, Okinaka, R T, Parson-Quintana, B, Reilly, L P, Richardson, P, Robinson, D L, Rubin, E, Saunders, E, Tapia, R, Tesmer, J G, Thayer, N, Thompson, L S, Tice, H, Ticknor, L O, Wills, P L, Gilna, P, & Brettin, T S. The Pathogenomic Sequence Analysis of B. cereus and B. Thuringiensis isolates closely related to Bacillus anthracis. United States.
Han, C S, Xie, G, Challacombe, J F, Altherr, M R, Bhotika, S S, Bruce, D, Campbell, C S, Campbell, M L, Chen, J, Chertkov, O, Cleland, C, Dimitrijevic-Bussod, M, Doggett, N A, Fawcett, J J, Glavina, T, Goodwin, L A, Hill, K K, Hitchcock, P, Jackson, P J, Keim, P, Kewalramani, A R, Longmire, J, Lucas, S, Malfatti, S, McMurry, K, Meincke, L J, Misra, M, Moseman, B L, Mundt, M, Munk, A C, Okinaka, R T, Parson-Quintana, B, Reilly, L P, Richardson, P, Robinson, D L, Rubin, E, Saunders, E, Tapia, R, Tesmer, J G, Thayer, N, Thompson, L S, Tice, H, Ticknor, L O, Wills, P L, Gilna, P, and Brettin, T S. Wed . "The Pathogenomic Sequence Analysis of B. cereus and B. Thuringiensis isolates closely related to Bacillus anthracis". United States. https://www.osti.gov/servlets/purl/885390.
@article{osti_885390,
title = {The Pathogenomic Sequence Analysis of B. cereus and B. Thuringiensis isolates closely related to Bacillus anthracis},
author = {Han, C S and Xie, G and Challacombe, J F and Altherr, M R and Bhotika, S S and Bruce, D and Campbell, C S and Campbell, M L and Chen, J and Chertkov, O and Cleland, C and Dimitrijevic-Bussod, M and Doggett, N A and Fawcett, J J and Glavina, T and Goodwin, L A and Hill, K K and Hitchcock, P and Jackson, P J and Keim, P and Kewalramani, A R and Longmire, J and Lucas, S and Malfatti, S and McMurry, K and Meincke, L J and Misra, M and Moseman, B L and Mundt, M and Munk, A C and Okinaka, R T and Parson-Quintana, B and Reilly, L P and Richardson, P and Robinson, D L and Rubin, E and Saunders, E and Tapia, R and Tesmer, J G and Thayer, N and Thompson, L S and Tice, H and Ticknor, L O and Wills, P L and Gilna, P and Brettin, T S},
abstractNote = {The sequencing and analysis of two close relatives of Bacillus anthracis are reported. AFLP analysis of over 300 isolates of B. cereus, B. thuringiensis and B. anthracis identified two isolates as being very closely related to B. anthracis. One, a B. cereus, BcE33L, was isolated from a zebra carcass in Nambia; the second, a B. thuringiensis, 97-27, was isolated from a necrotic human wound. The B. cereus appears to be the closest anthracis relative sequenced to date. A core genome of over 3,900 genes was compiled for the Bacillus cereus group, including B anthracis. Comparative analysis of these two genomes with other members of the B. cereus group provides insight into the evolutionary relationships among these organisms. Evidence is presented that differential regulation modulates virulence, rather than simple acquisition of virulence factors. These genome sequences provide insight into the molecular mechanisms contributing to the host range and virulence of this group of organisms.},
doi = {},
journal = {Journal of Bacteriology},
issn = {0021-9193},
number = 9,
volume = 180,
place = {United States},
year = {2005},
month = {10}
}