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Title: Method And System For Examining Biological Materials Using Low Power Cw Excitation Raman Spectroscopy.

Abstract

A method and system for examining biological materials using low-power cw excitation Raman spectroscopy. In accordance with the teachings of the invention, a low-power continuous wave (cw) pump laser beam and a low-power cw Stokes (or anti-Stokes) probe laser beam simultaneously illuminate a biological material and traverse the biological material in collinearity. The pump beam, whose frequency is varied, is used to induce Raman emission from the biological material. The intensity of the probe beam, whose frequency is kept constant, is monitored as it leaves the biological material. When the difference between the pump and probe excitation frequencies is equal to a Raman vibrational mode frequency of the biological material, the weak probe signal becomes amplified by one or more orders of magnitude (typically up to about 10.sup.4 -10.sup.6) due to the Raman emission from the pump beam. In this manner, by monitoring the intensity of the probe beam emitted from the biological material as the pump beam is varied in frequency, one can obtain an excitation Raman spectrum for the biological material tested. The present invention may be applied to in the in vivo and/or in vitro diagnosis of diabetes, heart disease, hepatitis, cancers and other diseases by measuringmore » the characteristic excitation Raman lines of blood glucose, cholesterol, serum glutamic oxalacetic transaminase (SGOT)/serum glutamic pyruvic tansaminase (SGPT), tissues and other corresponding Raman-active body constituents, respectively. For example, it may also be used to diagnose diseases associated with the concentration of Raman-active constituents in urine, lymph and saliva It may be used to identify cancer in the breast, cervix, uterus, ovaries and the like by measuring the fingerprint excitation Raman spectra of these tissues. It may also be used to reveal the growing of tumors or cancers by measuring the levels of nitric oxide in tissue.« less

Inventors:
 [1];  [2]
  1. (Bronx, NY)
  2. (Flushing, NY)
Publication Date:
Research Org.:
CITY UNIV OF NEW YORK RES FDN
OSTI Identifier:
879580
Patent Number(s):
US 6151522
Application Number:
09/270383
Assignee:
The Research Foundation of CUNY (New York, NY) CHO
DOE Contract Number:  
FG02-98ER62560
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Alfano, Robert R., and Wang, Wubao. Method And System For Examining Biological Materials Using Low Power Cw Excitation Raman Spectroscopy.. United States: N. p., 2000. Web.
Alfano, Robert R., & Wang, Wubao. Method And System For Examining Biological Materials Using Low Power Cw Excitation Raman Spectroscopy.. United States.
Alfano, Robert R., and Wang, Wubao. Tue . "Method And System For Examining Biological Materials Using Low Power Cw Excitation Raman Spectroscopy.". United States. https://www.osti.gov/servlets/purl/879580.
@article{osti_879580,
title = {Method And System For Examining Biological Materials Using Low Power Cw Excitation Raman Spectroscopy.},
author = {Alfano, Robert R. and Wang, Wubao},
abstractNote = {A method and system for examining biological materials using low-power cw excitation Raman spectroscopy. In accordance with the teachings of the invention, a low-power continuous wave (cw) pump laser beam and a low-power cw Stokes (or anti-Stokes) probe laser beam simultaneously illuminate a biological material and traverse the biological material in collinearity. The pump beam, whose frequency is varied, is used to induce Raman emission from the biological material. The intensity of the probe beam, whose frequency is kept constant, is monitored as it leaves the biological material. When the difference between the pump and probe excitation frequencies is equal to a Raman vibrational mode frequency of the biological material, the weak probe signal becomes amplified by one or more orders of magnitude (typically up to about 10.sup.4 -10.sup.6) due to the Raman emission from the pump beam. In this manner, by monitoring the intensity of the probe beam emitted from the biological material as the pump beam is varied in frequency, one can obtain an excitation Raman spectrum for the biological material tested. The present invention may be applied to in the in vivo and/or in vitro diagnosis of diabetes, heart disease, hepatitis, cancers and other diseases by measuring the characteristic excitation Raman lines of blood glucose, cholesterol, serum glutamic oxalacetic transaminase (SGOT)/serum glutamic pyruvic tansaminase (SGPT), tissues and other corresponding Raman-active body constituents, respectively. For example, it may also be used to diagnose diseases associated with the concentration of Raman-active constituents in urine, lymph and saliva It may be used to identify cancer in the breast, cervix, uterus, ovaries and the like by measuring the fingerprint excitation Raman spectra of these tissues. It may also be used to reveal the growing of tumors or cancers by measuring the levels of nitric oxide in tissue.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {11}
}

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