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Title: Combinatorial Screening Of Inorganic And Organometallic Materials

Abstract

A method for differentiating and enumerating nucleated red blood cells in a blood sample is described. The method includes the steps of lysing red blood cells of a blood sample with a lytic reagent, measuring nucleated blood cells by DC impedance measurement in a non-focused flow aperture, differentiating nucleated red blood cells from other cell types, and reporting nucleated red blood cells in the blood sample. The method further includes subtracting nucleated red blood cells and other interference materials from the count of remaining blood cells, and reporting a corrected white blood cell count of the blood sample. Additionally, the method further includes measuring spectrophotometric absorbance of the sample mixture at a predetermined wavelength of a hemoglobin chromogen formed upon lysing the blood sample, and reporting hemoglobin concentration of the blood sample.

Inventors:
 [1]
  1. (Miami, FL), Li, Jing (Miami, FL), Britton, Ted W. (Sunrise, FL)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
OSTI Identifier:
879545
Patent Number(s):
US 6410330
Application Number:
09/122932
Assignee:
Coulter International Corp. (Miami, FL)
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Li, Yi. Combinatorial Screening Of Inorganic And Organometallic Materials. United States: N. p., 2002. Web.
Li, Yi. Combinatorial Screening Of Inorganic And Organometallic Materials. United States.
Li, Yi. Tue . "Combinatorial Screening Of Inorganic And Organometallic Materials". United States. https://www.osti.gov/servlets/purl/879545.
@article{osti_879545,
title = {Combinatorial Screening Of Inorganic And Organometallic Materials},
author = {Li, Yi},
abstractNote = {A method for differentiating and enumerating nucleated red blood cells in a blood sample is described. The method includes the steps of lysing red blood cells of a blood sample with a lytic reagent, measuring nucleated blood cells by DC impedance measurement in a non-focused flow aperture, differentiating nucleated red blood cells from other cell types, and reporting nucleated red blood cells in the blood sample. The method further includes subtracting nucleated red blood cells and other interference materials from the count of remaining blood cells, and reporting a corrected white blood cell count of the blood sample. Additionally, the method further includes measuring spectrophotometric absorbance of the sample mixture at a predetermined wavelength of a hemoglobin chromogen formed upon lysing the blood sample, and reporting hemoglobin concentration of the blood sample.},
doi = {},
url = {https://www.osti.gov/biblio/879545}, journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {6}
}

Patent:

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