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Title: Low-LET Microbeam Investigation of the Track-End Dependence of Electron-Induced Damage in Normal Human Diploid Fibroblasts

Abstract

Using the electron microbeam located at Pacific Northwest National Laboratory, we have investigated the incident electron energy dependence on micronuclei formation in human diploid fibroblasts, AG01522, following non-targeted irradiations at 25 and 80 keV. Examining the dose response, we find that 25 keV electrons are more effective than 80 keV electrons at producing biological damage for a given dose. Our results demonstrating the induction of micronuclei as a function of incident electron energy offer direct support for the hypothesis that the track end is responsible for the biological damage occurring in the cell.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
876871
Report Number(s):
PNNL-SA-42273
Journal ID: ISSN 0033-7587; RAREAE; 6697; KP1303000; TRN: US0601526
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Radiation Research; Journal Volume: 164; Journal Issue: 5
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; ELECTRON BEAMS; ENERGY DEPENDENCE; FIBROBLASTS; BIOLOGICAL RADIATION EFFECTS; HUMAN POPULATIONS; DOSE-RESPONSE RELATIONSHIPS; Environmental Molecular Sciences Laboratory

Citation Formats

Sowa, Marianne B., Kathmann, Loel E., Holben, Brooke A., Thrall, Brian D., and Kimmel, Greg A.. Low-LET Microbeam Investigation of the Track-End Dependence of Electron-Induced Damage in Normal Human Diploid Fibroblasts. United States: N. p., 2005. Web. doi:10.1667/RR3464.1.
Sowa, Marianne B., Kathmann, Loel E., Holben, Brooke A., Thrall, Brian D., & Kimmel, Greg A.. Low-LET Microbeam Investigation of the Track-End Dependence of Electron-Induced Damage in Normal Human Diploid Fibroblasts. United States. doi:10.1667/RR3464.1.
Sowa, Marianne B., Kathmann, Loel E., Holben, Brooke A., Thrall, Brian D., and Kimmel, Greg A.. Tue . "Low-LET Microbeam Investigation of the Track-End Dependence of Electron-Induced Damage in Normal Human Diploid Fibroblasts". United States. doi:10.1667/RR3464.1.
@article{osti_876871,
title = {Low-LET Microbeam Investigation of the Track-End Dependence of Electron-Induced Damage in Normal Human Diploid Fibroblasts},
author = {Sowa, Marianne B. and Kathmann, Loel E. and Holben, Brooke A. and Thrall, Brian D. and Kimmel, Greg A.},
abstractNote = {Using the electron microbeam located at Pacific Northwest National Laboratory, we have investigated the incident electron energy dependence on micronuclei formation in human diploid fibroblasts, AG01522, following non-targeted irradiations at 25 and 80 keV. Examining the dose response, we find that 25 keV electrons are more effective than 80 keV electrons at producing biological damage for a given dose. Our results demonstrating the induction of micronuclei as a function of incident electron energy offer direct support for the hypothesis that the track end is responsible for the biological damage occurring in the cell.},
doi = {10.1667/RR3464.1},
journal = {Radiation Research},
number = 5,
volume = 164,
place = {United States},
year = {Tue Nov 01 00:00:00 EST 2005},
month = {Tue Nov 01 00:00:00 EST 2005}
}