Direct Fabrication of Enzyme-Carrying Polymer Nanofibers by Electrospinning
Nanofibers consisting of enzyme–polymer composites have been prepared by directly electrospinning a solution of surfactant-stabilized enzyme and polymer in toluene. Additional treatment with glutaraldehyde could greatly stabilize the enzyme activity of the fibers, which could be maintained in a buffer under shaking conditions for more than two weeks. We found that the nanofibers also showed great improvement in the enzyme activity over bulk films as a result of increased mass-transfer for substrate molecules to and from the enzyme reactive sites. Moreover, these stable and catalytically active nanofiber-based mats were highly durable and could be easily recovered from solution, making them ideal candidates for large scale applications.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 876868
- Report Number(s):
- PNNL-SA-42024; JMACEP; 6695; 3241; KP1301020; 400403809; TRN: US200608%%322
- Journal Information:
- Journal of Materials Chemistry, Vol. 15, Issue 31; ISSN 0959-9428
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
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