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Title: Examining microarray slide quality for the EPA using SNL's hyperspectral microarray scanner.

Abstract

This report summarizes research performed at Sandia National Laboratories (SNL) in collaboration with the Environmental Protection Agency (EPA) to assess microarray quality on arrays from two platforms of interest to the EPA. Custom microarrays from two novel, commercially produced array platforms were imaged with SNL's unique hyperspectral imaging technology and multivariate data analysis was performed to investigate sources of emission on the arrays. No extraneous sources of emission were evident in any of the array areas scanned. This led to the conclusions that either of these array platforms could produce high quality, reliable microarray data for the EPA toxicology programs. Hyperspectral imaging results are presented and recommendations for microarray analyses using these platforms are detailed within the report.

Authors:
;
Publication Date:
Research Org.:
Sandia National Laboratories
Sponsoring Org.:
USDOE
OSTI Identifier:
875988
Report Number(s):
SAND2005-7536
TRN: US200604%%219
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; 54 ENVIRONMENTAL SCIENCES; IMAGES; QUALITY ASSURANCE; DATA ANALYSIS; SANDIA NATIONAL LABORATORIES; US EPA; MULTIVARIATE ANALYSIS; EMISSION; TOXIC MATERIALS; Imaging systems.; Spectral data.; Spectroscopic imaging.; Microstructure.

Citation Formats

Rohde, Rachel M., and Timlin, Jerilyn Ann. Examining microarray slide quality for the EPA using SNL's hyperspectral microarray scanner.. United States: N. p., 2005. Web. doi:10.2172/875988.
Rohde, Rachel M., & Timlin, Jerilyn Ann. Examining microarray slide quality for the EPA using SNL's hyperspectral microarray scanner.. United States. doi:10.2172/875988.
Rohde, Rachel M., and Timlin, Jerilyn Ann. Tue . "Examining microarray slide quality for the EPA using SNL's hyperspectral microarray scanner.". United States. doi:10.2172/875988. https://www.osti.gov/servlets/purl/875988.
@article{osti_875988,
title = {Examining microarray slide quality for the EPA using SNL's hyperspectral microarray scanner.},
author = {Rohde, Rachel M. and Timlin, Jerilyn Ann},
abstractNote = {This report summarizes research performed at Sandia National Laboratories (SNL) in collaboration with the Environmental Protection Agency (EPA) to assess microarray quality on arrays from two platforms of interest to the EPA. Custom microarrays from two novel, commercially produced array platforms were imaged with SNL's unique hyperspectral imaging technology and multivariate data analysis was performed to investigate sources of emission on the arrays. No extraneous sources of emission were evident in any of the array areas scanned. This led to the conclusions that either of these array platforms could produce high quality, reliable microarray data for the EPA toxicology programs. Hyperspectral imaging results are presented and recommendations for microarray analyses using these platforms are detailed within the report.},
doi = {10.2172/875988},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Nov 01 00:00:00 EST 2005},
month = {Tue Nov 01 00:00:00 EST 2005}
}

Technical Report:

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