System and method for a parallel immunoassay system

Patent · Tue Jan 01 00:00:00 EST 2002

OSTI ID:874915

Stevens, Fred J ^[1]

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1. Naperville, IL

A method and system for detecting a target antigen using massively parallel immunoassay technology. In this system, high affinity antibodies of the antigen are covalently linked to small beads or particles. The beads are exposed to a solution containing DNA-oligomer-mimics of the antigen. The mimics which are reactive with the covalently attached antibody or antibodies will bind to the appropriate antibody molecule on the bead. The particles or beads are then washed to remove any unbound DNA-oligomer-mimics and are then immobilized or trapped. The bead-antibody complexes are then exposed to a test solution which may contain the targeted antigens. If the antigen is present it will replace the mimic since it has a greater affinity for the respective antibody. The particles are then removed from the solution leaving a residual solution. This residual solution is applied a DNA chip containing many samples of complimentary DNA. If the DNA tag from a mimic binds with its complimentary DNA, it indicates the presence of the target antigen. A flourescent tag can be used to more easily identify the bound DNA tag.

• Research Organization: Argonne National Laboratory (ANL), Argonne, IL (United States)
• DOE Contract Number: W-31109-ENG-38
• Assignee: The United States of America as represented by the United States Department (Washington, DC)
• Patent Number(s): US 6489120
• OSTI ID: 874915
• Country of Publication: United States
• Language: English

Related Subjects

method
parallel
immunoassay
detecting
target
antigen
massively
technology
affinity
antibodies
covalently
linked
beads
particles
exposed
solution
containing
dna-oligomer-mimics
mimics
reactive
attached
antibody
bind
appropriate
molecule
bead
washed
remove
unbound
immobilized
trapped
bead-antibody
complexes
contain
targeted
antigens
replace
mimic
removed
leaving
residual
applied
dna
chip
samples
complimentary
tag
binds
indicates
presence
flourescent
easily
identify
bound
massively parallel
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