Promoter sequence of 3-phosphoglycerate kinase gene 1 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species
Patent
·
OSTI ID:874806
- Richland, WA
- Pendleton, OR
The present invention provides the promoter clone discovery of phosphoglycerate kinase gene 1 of a lactic acid-producing filamentous fungal strain, Rhizopus oryzae. The isolated promoter can constitutively regulate gene expression under various carbohydrate conditions. In addition, the present invention also provides a design of an integration vector for the transformation of a foreign gene in Rhizopus oryzae.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC06-76RL01830
- Assignee:
- Battelle Memorial Institute (Richland, WA)
- Patent Number(s):
- 6,465,635
- OSTI ID:
- 874806
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
promoter
sequence
3-phosphoglycerate
kinase
lactic
acid-producing
fungus
rhizopus
oryzae
method
expressing
fungal
species
provides
clone
discovery
phosphoglycerate
filamentous
strain
isolated
constitutively
regulate
expression
carbohydrate
conditions
addition
design
integration
vector
transformation
foreign
lactic acid
promoter sequence
/999/435/
sequence
3-phosphoglycerate
kinase
lactic
acid-producing
fungus
rhizopus
oryzae
method
expressing
fungal
species
provides
clone
discovery
phosphoglycerate
filamentous
strain
isolated
constitutively
regulate
expression
carbohydrate
conditions
addition
design
integration
vector
transformation
foreign
lactic acid
promoter sequence
/999/435/