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Title: Promoter sequence of 3-phosphoglycerate kinase gene 1 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species

Patent ·
OSTI ID:874806

The present invention provides the promoter clone discovery of phosphoglycerate kinase gene 1 of a lactic acid-producing filamentous fungal strain, Rhizopus oryzae. The isolated promoter can constitutively regulate gene expression under various carbohydrate conditions. In addition, the present invention also provides a design of an integration vector for the transformation of a foreign gene in Rhizopus oryzae.

Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC06-76RL01830
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Number(s):
6,465,635
OSTI ID:
874806
Country of Publication:
United States
Language:
English

References (12)

Transformation of Filamentous Fungi with Plasmid DNA by Electroporation journal January 1989
Isolation and identification of yeast messenger ribonucleic acids coding for enolase, glyceraldehyde-3-phosphate dehydrogenase, and phosphoglycerate kinase journal November 1978
Sequence of the Penicillium chrysogenum phosphoglycerate kinase gene journal January 1988
Cloning and characterization of two 3-phosphoglycerate kinase genes of Rhizopus niveus and heterologous gene expression using their promoters journal June 1994
The primary structure of the Saccharomyces cerevisiae gene for 3-phosphogrycerate kinase journal January 1982
Efficient synthesis of enzymatically active calf chymosin in Saccharomyces cerevisiae journal September 1983
Analysis of the 3-phosphoglycerate kinase 2 promoter in Rhizopus niveus journal January 1995
Gene Transfer by Electroporation of Filamentous Fungi book June 1995
Promoter structure and expression of the 3-phosphoglycerate kinase-encoding gene (pgk1) of Trichoderma reesei journal September 1991
Cloning, expression and characterization of a cDNA encoding a lipase from Rhizopus delemar journal December 1991
Production of l-lactic acid by Rhizopus species journal July 1994
Enhanced production of l(+)-lactic acid from corn starch in a culture of Rhizopus oryzae using an air-lift bioreactor journal January 1997