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Title: Detection of nucleic acids by multiple sequential invasive cleavages 02

Abstract

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.

Inventors:
 [1];  [1];  [1];  [1]
  1. Madison, WI
Publication Date:
Research Org.:
Third Wave Technologies, Inc., Madison, WI (United States)
OSTI Identifier:
874777
Patent Number(s):
US 6458535
Assignee:
Third Wave Technologies, Inc (Madison, WI)
DOE Contract Number:  
FG02-94ER81891
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
detection; nucleic; acids; multiple; sequential; invasive; cleavages; 02; relates; means; characterization; acid; sequences; variations; methods; forming; cleavage; structure; target; sequence; cleaving; site-specific; manner; structure-specific; nuclease; activity; variety; enzymes; cleave; target-dependent; indicating; presence; specific; devices; separation; molecules; based; charge; provides; non-target; products; via; formation; complete; activated; protein; binding; region; sensitive; human; cytomegalovirus; sample; nucleic acid; acid sequence; specific nucleic; /435/

Citation Formats

Hall, Jeff G, Lyamichev, Victor I, Mast, Andrea L, and Brow, Mary Ann D. Detection of nucleic acids by multiple sequential invasive cleavages 02. United States: N. p., 2002. Web.
Hall, Jeff G, Lyamichev, Victor I, Mast, Andrea L, & Brow, Mary Ann D. Detection of nucleic acids by multiple sequential invasive cleavages 02. United States.
Hall, Jeff G, Lyamichev, Victor I, Mast, Andrea L, and Brow, Mary Ann D. Tue . "Detection of nucleic acids by multiple sequential invasive cleavages 02". United States. https://www.osti.gov/servlets/purl/874777.
@article{osti_874777,
title = {Detection of nucleic acids by multiple sequential invasive cleavages 02},
author = {Hall, Jeff G and Lyamichev, Victor I and Mast, Andrea L and Brow, Mary Ann D},
abstractNote = {The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}

Patent:

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