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Title: Expression of multiple proteins in transgenic plants

Abstract

A method is disclosed for the production of multiple proteins in transgenic plants. A DNA construct for introduction into plants includes a provision to express a fusion protein of two proteins of interest joined by a linking domain including plant ubiquitin. When the fusion protein is produced in the cells of a transgenic plant transformed with the DNA construction, native enzymes present in plant cells cleave the fusion protein to release both proteins of interest into the cells of the transgenic plant. Since the proteins are produced from the same fusion protein, the initial quantities of the proteins in the cells of the plant are approximately equal.

Inventors:
 [1];  [1]
  1. (Madison, WI)
Publication Date:
Research Org.:
The Consortium for Plant Biotechnology Research, Inc., West Lafayette, IN (US)
OSTI Identifier:
874759
Patent Number(s):
US 6455759
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI) ORO
DOE Contract Number:  
FC05-92OR22072
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
expression; multiple; proteins; transgenic; plants; method; disclosed; production; dna; construct; introduction; provision; express; fusion; protein; joined; linking; domain; including; plant; ubiquitin; produced; cells; transformed; construction; native; enzymes; cleave; release; initial; quantities; approximately; equal; plant cell; dna construct; fusion protein; transgenic plant; /800/

Citation Formats

Vierstra, Richard D., and Walker, Joseph M. Expression of multiple proteins in transgenic plants. United States: N. p., 2002. Web.
Vierstra, Richard D., & Walker, Joseph M. Expression of multiple proteins in transgenic plants. United States.
Vierstra, Richard D., and Walker, Joseph M. Tue . "Expression of multiple proteins in transgenic plants". United States. https://www.osti.gov/servlets/purl/874759.
@article{osti_874759,
title = {Expression of multiple proteins in transgenic plants},
author = {Vierstra, Richard D. and Walker, Joseph M.},
abstractNote = {A method is disclosed for the production of multiple proteins in transgenic plants. A DNA construct for introduction into plants includes a provision to express a fusion protein of two proteins of interest joined by a linking domain including plant ubiquitin. When the fusion protein is produced in the cells of a transgenic plant transformed with the DNA construction, native enzymes present in plant cells cleave the fusion protein to release both proteins of interest into the cells of the transgenic plant. Since the proteins are produced from the same fusion protein, the initial quantities of the proteins in the cells of the plant are approximately equal.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 2002},
month = {Tue Jan 01 00:00:00 EST 2002}
}

Patent:

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