Replica amplification of nucleic acid arrays

Patent · Tue Jan 01 00:00:00 EST 2002

OSTI ID:874646

Church, George M ^[1]

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1. Brookline, MA

A method of producing a plurality of a nucleic acid array, comprising, in order, the steps of amplifying in situ nucleic acid molecules of a first randomly-patterned, immobilized nucleic acid array comprising a heterogeneous pool of nucleic acid molecules affixed to a support, transferring at least a subset of the nucleic acid molecules produced by such amplifying to a second support, and affixing the subset so transferred to the second support to form a second randomly-patterned, immobilized nucleic acid array, wherein the nucleic acid molecules of the second array occupy positions that correspond to those of the nucleic acid molecules from which they were amplified on the first array, so that the first array serves as a template to produce a plurality, is disclosed.

• Research Organization: Harvard University
• DOE Contract Number: FG02-87ER60565
• Assignee: President and Fellows of Harvard College (Cambridge, MA)
• Patent Number(s): US 6432360
• OSTI ID: 874646
• Country of Publication: United States
• Language: English

Related Subjects

replica
amplification
nucleic
acid
arrays
method
producing
plurality
array
comprising
steps
amplifying
situ
molecules
randomly-patterned
immobilized
heterogeneous
pool
affixed
support
transferring
subset
produced
affixing
transferred
form
occupy
positions
amplified
serves
template
produce
disclosed
nucleic acid
acid molecule
/422/435/999/