Methods and compositions for efficient nucleic acid sequencing

Patent · 01 January 2002

OSTI ID:874493

Drmanac, Radoje ^[1]

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1. 850 E. Greenwich Pl., Palo Alto, CA 94303

Disclosed are novel methods and compositions for rapid and highly efficient nucleic acid sequencing based upon hybridization with two sets of small oligonucleotide probes of known sequences. Extremely large nucleic acid molecules, including chromosomes and non-amplified RNA, may be sequenced without prior cloning or subcloning steps. The methods of the invention also solve various current problems associated with sequencing technology such as, for example, high noise to signal ratios and difficult discrimination, attaching many nucleic acid fragments to a surface, preparing many, longer or more complex probes and labelling more species.

• Research Organization: Argonne National Laboratory (ANL), Argonne, IL
• DOE Contract Number: W-31109-ENG-38
• Assignee: Drmanac; Radoje (850 E. Greenwich Pl., Palo Alto, CA 94303)
• Patent Number(s): US 6401267
• OSTI ID: 874493
• Country of Publication: United States
• Language: English

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