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Method and apparatus for synthesis of arrays of DNA probes

Patent ·
OSTI ID:874365
 [1];  [1];  [1];  [1];  [1]
  1. Madison, WI
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The synthesis of arrays of DNA probes sequences, polypeptides, and the like is carried out using a patterning process on an active surface of a substrate. An image is projected onto the active surface of the substrate utilizing an image former that includes a light source that provides light to a micromirror device comprising an array of electronically addressable micromirrors, each of which can be selectively tilted between one of at least two positions. Projection optics receives the light reflected from the micromirrors along an optical axis and precisely images the micromirrors onto the active surface of the substrate, which may be used to activate the surface of the substrate. The first level of bases may then be applied to the substrate, followed by development steps, and subsequent exposure of the substrate utilizing a different pattern of micromirrors, with further repeats until the elements of a two dimensional array on the substrate surface have an appropriate base bound thereto. The micromirror array can be controlled in conjunction with a DNA synthesizer supplying appropriate reagents to a flow cell containing the active substrate to control the sequencing of images presented by the micromirror array in coordination of the reagents provided to the substrate.

Research Organization:
University of Wisconsin
DOE Contract Number:
FG02-96ER45569
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI)
Patent Number(s):
US 6375903
Application Number:
09/253,460
OSTI ID:
874365
Country of Publication:
United States
Language:
English

References (9)

Broadband Deep-UV High NA Photolithography System conference July 1989
The Efficiency of Light-Directed Synthesis of DNA Arrays on Glass Substrates
  • McGall, Glenn H.; Barone, Anthony D.; Diggelmann, Martin
  • Journal of the American Chemical Society, Vol. 119, Issue 22, p. 5081-5090 https://doi.org/10.1021/ja964427a
journal June 1997
Light-generated oligonucleotide arrays for rapid DNA sequence analysis. journal May 1994
Light-directed synthesis of high-density oligonucleotide arrays using semiconductor photoresists journal November 1996
Excimer Laser Photolithography With 1:1 Wynne-Dyson Optics conference January 1988
New Concepts in Projection Mask Aligners journal April 1975
Two-dimensional spatial light modulators: a tutorial journal May 1990
Digital Light Processing and MEMS: reflecting the digital display needs of the networked society conference August 1996
Excimer laser projection lithography on a full-field scanning projection system journal May 1986

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