Apparatus and method for collection and concentration of respirable particles into a small fluid volume

Patent · Tue Jan 01 00:00:00 EST 2002

OSTI ID:874197

Simon, Jonathan N ^[1]; Brown, Steve B ^[2]

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1. San Leandro, CA
2. Livermore, CA

An apparatus and method for the collection of respirable particles and concentration of such particles into a small fluid volume. The apparatus captures and concentrates small (1-10 .mu.m) respirable particles into a sub-millileter volume of fluid. The method involves a two step operation, collection and concentration: wherein collection of particles is by a wetted surface having small vertical slits that act as capillary channels; and concentration is carried out by transfer of the collected particles to a small volume (sub-milliliter) container by centrifugal force whereby the particles are forced through the vertical slits and contact a non-wetted wall surface, and are deflected to the bottom where they are contained for analysis, such as a portable flow cytometer or a portable PCR DNA analysis system.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
• DOE Contract Number: W-7405-ENG-48
• Assignee: The Regents of the University of California (Oakland, CA)
• Patent Number(s): US 6337213
• OSTI ID: 874197
• Country of Publication: United States
• Language: English

Related Subjects

apparatus
method
collection
concentration
respirable
particles
fluid
volume
captures
concentrates
1-10
mum
sub-millileter
involves
step
operation
wetted
surface
vertical
slits
capillary
channels
carried
transfer
collected
sub-milliliter
container
centrifugal
force
forced
contact
non-wetted
wall
deflected
bottom
contained
analysis
portable
flow
cytometer
pcr
dna
centrifugal force
fluid volume
/436/55/422/