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Production of amino acids using auxotrophic mutants of methylotrophic bacillus

Patent ·
OSTI ID:873858
A method of producing amino acids by culturing an amino acid auxotroph of a biologically pure strain of a type I methylotrophic bacterium of the genus Bacillus which exhibits sustained growth at 50.degree. C. using methanol as a carbon and energy source and requiring vitamin B.sub.12 and biotin is provided.
Research Organization:
UNIVERSITY OF MINNESOTA
DOE Contract Number:
AC02-82ER12029
Assignee:
Regents of University of Minnesota (Minneapolis, MN)
Patent Number(s):
US 6261825
Application Number:
07/673,264
OSTI ID:
873858
Country of Publication:
United States
Language:
English

References (22)

The Isolation and Characterization of Mutants of Bacillus subtilis and Bacillus licheniformis with Disturbed Morphology and Cell Division journal May 1970
Exospores and Cysts Formed by Methane-utilizing Bacteria journal May 1970
The production of l-lysine by fermentation journal July 1983
Cellular Permeability and Extracellular Formation of Glutamic Acid in Brevibacterium flavum journal May 1963
L-lysine production at 50 degrees C by mutants of a newly isolated and characterized methylotrophic Bacillus sp. journal January 1990
The Prokaryotic Transposable Element Tn5 journal July 1983
Effect of Biotin on the Bacterial Formation of Glutamic Acid journal January 1962
Environmental control of metabolic fluxes in thermotolerant methylotrophic Bacillus strains journal February 1989
Rapid method for distinction of gram-negative from gram-positive bacteria journal January 1978
Nocardia sp. 239, a facultative methanol utilizer with the ribulose monophosphate pathway of formaldehyde fixation journal September 1983
A Broad Host Range Mobilization System for In Vivo Genetic Engineering Transposon Mutagenesis in Gram Negative Bacteria journal November 1983
Hexose phosphate synthase in trimethylamine-grown bacterium 2B2, a facultative methylotroph journal August 1974
Isolation and initial characterization of thermotolerant methylotrophic Bacillus strains journal August 1988
Microbial growth on C1 compounds. 5. Enzyme activities in extracts of Pseudomonas AM1 journal May 1963
Biochemical Effects of Fatty Acid and its Derivatives on L-Glutamic Acid Fermentation journal January 1964
Effect of Penicillin on Amino Acid Fermentation journal January 1964
[109] Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA book January 1968
Colorimetric Assay for Dipicolinic Acid in Bacterial Spores journal January 1958
Ecology and Diversity of Methylotrophic Organisms book January 1980
Identification of upstream regulatory elements involved in the developmental expression of the Arabidopsis thaliana cab1 gene. journal November 1988
Thermophilic Mixed Culture of Bacteria Utilizing Methanol for Growth1 journal January 1974
Reaction of ninhydrin in acid solution with straight-chain amino acids containing two amino groups and its application to the estimation of α∈-diaminopimelic acid journal November 1957