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Title: Nucleic acid indexing

Patent ·
OSTI ID:873713
 [1];  [2]
  1. Madison, WI
  2. Bellevue, WA
+ Show Author Affiliations

A restriction site indexing method for selectively amplifying any fragment generated by a Class II restriction enzyme includes adaptors specific to fragment ends containing adaptor indexing sequences complementary to fragment indexing sequences near the termini of fragments generated by Class II enzyme cleavage. A method for combinatorial indexing facilitates amplification of restriction fragments whose sequence is not known.

Research Organization:
Univ. of Wisconsin, Madison, WI (United States)
DOE Contract Number:
FG02-91ER61122
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI)
Patent Number(s):
US 6228999
OSTI ID:
873713
Country of Publication:
United States
Language:
English

References (14)

Branch capture reactions: effect of recipient structure journal January 1990
Covalently linked sequencing primer (splinkers) for sequence analysis of restriction fragments journal January 1986
"RFLP subtraction": a method for making libraries of polymorphic markers. journal June 1994
[19] Representational difference analysis in detection of genetic lesions in cancer book January 1995
Branch capture reactions: displacers derived from asymmetric PCR journal January 1991
Non-cloning amplification of specific DNA fragments from whole genomic DNA digests using DNA ‘indexers’ journal August 1994
Ligation-mediated PCR of restriction fragments from large DNA molecules. journal August 1992
Cloning the differences between two complex genomes journal February 1993
Corrigendum journal June 1993
[18] Fingerprinting of DNA and RNA by arbitrarily primed polymerase chain reaction: Applications in cancer research book January 1995
AFLP: a new technique for DNA fingerprinting journal January 1995
Branch migration mediated DNA labeling and cloning journal October 1989
Ligation-mediated PCR amplification of specific fragments from a Class-II restriction endonuclease total digest journal May 1997
[20] Analysis of altered gene expression by differential display book January 1995

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Related Subjects

nucleic
acid
indexing
restriction
site
method
selectively
amplifying
fragment
generated
ii
enzyme
adaptors
specific
containing
adaptor
sequences
complementary
near
termini
fragments
cleavage
combinatorial
facilitates
amplification
sequence
nucleic acid
restriction enzyme
restriction fragments
restriction site
indexing sequences
selectively amplifying
sequences complementary
site indexing
fragments generated
fragment generated
fragment indexing
indexing method
ii restriction
restriction fragment
acid indexing
adaptors specific
adaptor indexing
containing adaptor
/999/435/