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Title: Recombinant pinoresinol/lariciresinol reductase, recombinant dirigent protein, and methods of use

Abstract

Dirigent proteins and pinoresinol/lariciresinol reductases have been isolated, together with cDNAs encoding dirigent proteins and pinoresinol/lariciresinol reductases. Accordingly, isolated DNA sequences are provided which code for the expression of dirigent proteins and pinoresinol/lariciresinol reductases. In other aspects, replicable recombinant cloning vehicles are provided which code for dirigent proteins or pinoresinol/lariciresinol reductases or for a base sequence sufficiently complementary to at least a portion of dirigent protein or pinoresinol/lariciresinol reductase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding dirigent protein or pinoresinol/lariciresinol reductase. Thus, systems and methods are provided for the recombinant expression of dirigent proteins and/or pinoresinol/lariciresinol reductases.

Inventors:
 [1];  [1];  [2];  [3];  [4];  [5];  [1]
  1. (Pullman, WA)
  2. (Baltimore, MD)
  3. (Kagawa, JP)
  4. (Ann Arbor, MI)
  5. (S. Minneapolis, MN)
Publication Date:
Research Org.:
Washington State University Research Foundation (Pullman, Washington)
OSTI Identifier:
873645
Patent Number(s):
US 6210942
Application Number:
09/475316
Assignee:
Washington State University Research Foundation (Pullman, Washington); OAK
DOE Contract Number:  
FG03-97ER20259
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
recombinant; pinoresinol; lariciresinol; reductase; dirigent; protein; methods; proteins; reductases; isolated; cdnas; encoding; accordingly; dna; sequences; provided; code; expression; aspects; replicable; cloning; vehicles; base; sequence; sufficiently; complementary; portion; rna; enable; hybridization; therewith; modified; host; cells; transformed; transfected; infected; injected; vehicle; systems; sequence sufficiently; modified host; cloning vehicles; recombinant expression; replicable recombinant; enable hybridization; hybridization therewith; recombinant cloning; dna sequences; dna sequence; isolated dna; host cells; sequence encoding; base sequence; cloning vehicle; host cell; sufficiently complementary; cdnas encoding; dirigent proteins; lariciresinol reductase; dirigent protein; /435/999/

Citation Formats

Lewis, Norman G., Davin, Laurence B., Dinkova-Kostova, Albena T., Fujita, Masayuki, Gang, David R., Sarkanen, Simo, and Ford, Joshua D.. Recombinant pinoresinol/lariciresinol reductase, recombinant dirigent protein, and methods of use. United States: N. p., 2001. Web.
Lewis, Norman G., Davin, Laurence B., Dinkova-Kostova, Albena T., Fujita, Masayuki, Gang, David R., Sarkanen, Simo, & Ford, Joshua D.. Recombinant pinoresinol/lariciresinol reductase, recombinant dirigent protein, and methods of use. United States.
Lewis, Norman G., Davin, Laurence B., Dinkova-Kostova, Albena T., Fujita, Masayuki, Gang, David R., Sarkanen, Simo, and Ford, Joshua D.. Tue . "Recombinant pinoresinol/lariciresinol reductase, recombinant dirigent protein, and methods of use". United States. https://www.osti.gov/servlets/purl/873645.
@article{osti_873645,
title = {Recombinant pinoresinol/lariciresinol reductase, recombinant dirigent protein, and methods of use},
author = {Lewis, Norman G. and Davin, Laurence B. and Dinkova-Kostova, Albena T. and Fujita, Masayuki and Gang, David R. and Sarkanen, Simo and Ford, Joshua D.},
abstractNote = {Dirigent proteins and pinoresinol/lariciresinol reductases have been isolated, together with cDNAs encoding dirigent proteins and pinoresinol/lariciresinol reductases. Accordingly, isolated DNA sequences are provided which code for the expression of dirigent proteins and pinoresinol/lariciresinol reductases. In other aspects, replicable recombinant cloning vehicles are provided which code for dirigent proteins or pinoresinol/lariciresinol reductases or for a base sequence sufficiently complementary to at least a portion of dirigent protein or pinoresinol/lariciresinol reductase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding dirigent protein or pinoresinol/lariciresinol reductase. Thus, systems and methods are provided for the recombinant expression of dirigent proteins and/or pinoresinol/lariciresinol reductases.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Apr 03 00:00:00 EDT 2001},
month = {Tue Apr 03 00:00:00 EDT 2001}
}

Patent:

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