Modified pseudomonas oleovorans phaC1 nucleic acids encoding bispecific polyhydroxyalkanoate polymerase

Srienc, Friedrich; Jackson, John K; Somers, David A

Title: Modified pseudomonas oleovorans phaC1 nucleic acids encoding bispecific polyhydroxyalkanoate polymerase

Patent · Sat Jan 01 00:00:00 EST 2000

OSTI ID:873364

Srienc, Friedrich ^[1]; Jackson, John K ^[2]; Somers, David A ^[3]

Lake Elmo, MN
Plymouth, MN
Roseville, MN

A genetically engineered Pseudomonas oleovorans phaC1 polyhydroxyalkanoate (PHA) polymerase having tailored substrate specificity is provided. The modified PHA polymerase is preferably a "bispecific" PHA polymerase capable of copolymerizing a short chain length monomer and a medium chain length monomer is provided. Methods for making the modified PHA polymerase and for making nucleic acids encoding the modified PHA polymerase are also disclosed, as are methods of producing PHA using the modified PHA polymerase. The invention further includes methods to assay for altered substrate specificity.

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Research Organization:: The Consortium for Plant Biotechnology Research, Inc., West Lafayette, IN (US)

DOE Contract Number:: FC05-92OR22072

Assignee:: Regents of University of Minnesota (Minneapolis, MN)

Patent Number(s):: US 6143952

OSTI ID:: 873364

Country of Publication:: United States

Language:: English

References (36)

Production of a novel copolyester of 3-hydroxybutyric acid and medium-chain-length 3-hydroxyalkanoic acids by Pseudomonas sp. 61-3 from sugars Kato, M.; Bao, H. J.; Kang, C. -K. Applied Microbiology and Biotechnology, Vol. 45, Issue 3 https://doi.org/10.1007/s002530050697	journal	April 1996
Stable transformation of maize after gene transfer by electroporation Fromm, Michael E.; Taylor, Loverine P.; Walbot, Virginia Nature, Vol. 319, Issue 6056 https://doi.org/10.1038/319791a0	journal	February 1986
Functional expression of the PHA synthase gene C1 from in results in poly(3-hydroxyalkanoate) synthesis Langenbach, S.; Rehm, B.; Steinbuchel, A. FEMS Microbiology Letters, Vol. 150, Issue 2 https://doi.org/10.1016/S0378-1097(97)00142-0	journal	May 1997
Polyhydroxyalkanoic acids Steinbüchel, Alexander Biomaterials https://doi.org/10.1007/978-1-349-11167-1_3	book	January 1991
Growth Kinetics, Nutrient Uptake, and Expression of the Alcaligenes eutrophus Poly(β-hydroxybutyrate) Synthesis Pathway in Transgenic Maize Cell Suspension Cultures Hahn, J. J.; Eschenlauer, A. C.; Narrol, M. H. Biotechnology Progress, Vol. 13, Issue 4 https://doi.org/10.1021/bp970033r	journal	August 1997
Pseudomonas oleovorans as a Source of Poly(β-Hydroxyalkanoates) for Potential Applications as Biodegradable Polyesters Brandl, Helmut; Gross, Richard A.; Lenz, Robert W. Applied and Environmental Microbiology, Vol. 54, Issue 8 https://doi.org/10.1128/AEM.54.8.1977-1982.1988	journal	January 1988
Optimization of DNA shuffling for high fidelity recombination Zhao, H. Nucleic Acids Research, Vol. 25, Issue 6 https://doi.org/10.1093/nar/25.6.1307	journal	March 1997
Random recombination of antibody single chain Fv sequences after fragmentation with DNasel in the presence of Mn ²⁺ Lorimer, Ian A. J.; Pastan, Ira Nucleic Acids Research, Vol. 23, Issue 15 https://doi.org/10.1093/nar/23.15.3067	journal	January 1995
Expression of genes transferred into monocot and dicot plant cells by electroporation. Fromm, M.; Taylor, L. P.; Walbot, V. Proceedings of the National Academy of Sciences, Vol. 82, Issue 17, p. 5824-5828 https://doi.org/10.1073/pnas.82.17.5824	journal	September 1985
The importance of PHB-synthase substrate specificity in polyhydroxyalkanoate synthesis by Alcaligenes eutrophus Haywood, G. W.; Anderson, A. J.; Dawes, E. A. FEMS Microbiology Letters, Vol. 57, Issue 1 https://doi.org/10.1111/j.1574-6968.1989.tb03210.x	journal	January 1989
A Pseudomonas strain accumulating polyesters of 3-hydroxybutyric acid and medium-chain-length 3-hydroxyalkanoic acids Steinb�chel, Alexander; Wiese, Stefan Applied Microbiology and Biotechnology, Vol. 37, Issue 6 https://doi.org/10.1007/BF00174829	journal	September 1992
The carboxy-terminal end of glycolate oxidase directs a foreign protein into tobacco leaf peroxisomes. Volokita, M. The Plant Journal, Vol. 1, Issue 3 https://doi.org/10.1046/j.1365-313X.1991.t01-4-00999.x	journal	November 1991
Characterization of two 3-ketothiolases possessing differing substrate specificities in the polyhydroxyalkanoate synthesizing organism Alcaligenes eutrophus Haywood, G. W.; Anderson, A. J.; Chu, L. FEMS Microbiology Letters, Vol. 52, Issue 1-2 https://doi.org/10.1111/j.1574-6968.1988.tb02577.x	journal	July 1988
Staining method of poly(3-hydroxyalkanoic acids) producing bacteria by nile blue Kitamura, Shiro; Doi, Yoshihara Biotechnology Techniques, Vol. 8, Issue 5 https://doi.org/10.1007/BF02428979	journal	May 1994
Metabolism in Plant Peroxisomes Huang, Anthony H. C. Cellular and Subcellular Localization in Plant Metabolism https://doi.org/10.1007/978-1-4757-4727-0_3	book	January 1982
Production of poly(3-hydroxybutyric acid) by recombinant Escherichia coli strains: genetic and fermentation studies Lee, Sang Yup; Chang, Ho Nam Canadian Journal of Microbiology, Vol. 41, Issue 13 https://doi.org/10.1139/m95-189	journal	December 1995
Poly(β-hydroxyalkanoate) copolymers containing brominated repeating units produced by Pseudomonas oleovorans Kim, Young B.; Lenz, R. W.; Fuller, R. Clinton Macromolecules, Vol. 25, Issue 7 https://doi.org/10.1021/ma00033a002	journal	March 1992
Plastics from bacteria and for bacteria: Poly(β-hydroxyalkanoates) as natural, biocompatible, and biodegradable polyesters Brandl, Helmut; Gross, Richard A.; Lenz, Robert W. Microbial Bioproducts https://doi.org/10.1007/BFb0010232	book
Primary sequence of the Escherichia coli fadBA operon, encoding the fatty acid-oxidizing multienzyme complex, indicates a high degree of homology to eucaryotic enzymes. DiRusso, C. C. Journal of Bacteriology, Vol. 172, Issue 11 https://doi.org/10.1128/JB.172.11.6459-6468.1990	journal	January 1990
DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution. Stemmer, W. P. Proceedings of the National Academy of Sciences, Vol. 91, Issue 22 https://doi.org/10.1073/pnas.91.22.10747	journal	October 1994
Gas chromatographic determination of poly-β-hydroxybutyric acid in microbial biomass after hydrochloric acid propanolysis Riis, V.; Mai, W. Journal of Chromatography A, Vol. 445 https://doi.org/10.1016/S0021-9673(01)84535-0	journal	January 1988
Crystallization behavior and its influence on the mechanical properties of a thermoplastic elastomer produced by Pseudomonas oleovorans Gagnon, K. D.; Lenz, R. W.; Farris, R. J. Macromolecules, Vol. 25, Issue 14 https://doi.org/10.1021/ma00040a018	journal	July 1992
Fatty acid degradation in plant peroxisomes: Function and biosynthesis of the enzymes involved Kindl, H. Biochimie, Vol. 75, Issue 3-4 https://doi.org/10.1016/0300-9084(93)90080-C	journal	January 1993
Microbodies—Peroxisomes and Glyoxysomes Tolbert, N. E. The Plant Cell https://doi.org/10.1016/B978-0-12-675401-8.50015-4	book	January 1980
Microbodies in Higher Plants Beevers, H. Annual Review of Plant Physiology, Vol. 30, Issue 1 https://doi.org/10.1146/annurev.pp.30.060179.001111	journal	June 1979
Biogenesis of Peroxisomes Lazarow, P. B.; Fujiki, Y. Annual Review of Cell Biology, Vol. 1, Issue 1 https://doi.org/10.1146/annurev.cb.01.110185.002421	journal	November 1985
Genetically transformed maize plants from protoplasts Rhodes, C.; Pierce, D.; Mettler, I. Science, Vol. 240, Issue 4849 https://doi.org/10.1126/science.2832947	journal	April 1988
Saccharomyces cerevisiae expressing bacterial polyhydroxybutyrate synthase produces poly-3-hydroxybutyrate Leaf, T. A.; Peterson, M. S.; Stoup, S. K. Microbiology, Vol. 142, Issue 5 https://doi.org/10.1099/13500872-142-5-1169	journal	May 1996
Metabolism of poly(3-hydroxyalkanoates) (PHAs) by Pseudomonas oleovorans. Identification and sequences of genes and function of the encoded proteins in the synthesis and degradation of PHA Huisman, G. W.; Wonink, E.; Meima, R. Journal of Biological Chemistry, Vol. 266, Issue 4 https://doi.org/10.1016/S0021-9258(18)52227-4	journal	February 1991
Effect of nitrogen limitation on long-side-chain poly-beta-hydroxyalkanoate synthesis by Pseudomonas resinovorans. Ramsay, B. A.; Saracovan, I.; Ramsay, J. A. Applied and Environmental Microbiology, Vol. 58, Issue 2 https://doi.org/10.1128/AEM.58.2.744-746.1992	journal	January 1992
Formation of polyesters consisting of medium-chain-length 3-hydroxyalkanoic acids from gluconate by Pseudomonas aeruginosa and other fluorescent pseudomonads. Timm, A.; Steinbüchel, A. Applied and Environmental Microbiology, Vol. 56, Issue 11 https://doi.org/10.1128/AEM.56.11.3360-3367.1990	journal	January 1990
Plant Organellar Targeting Sequences Wallace, T. P.; Howe, C. J. Plant Molecular Biology Labfax https://doi.org/10.1016/B978-0-08-091753-5.50014-7	book	January 1993
Poly-β-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16 Peoples, O. P.; Sinskey, A. J. Journal of Biological Chemistry, Vol. 264, Issue 26 https://doi.org/10.1016/S0021-9258(19)84825-1	journal	September 1989
Production of heteropolymeric polyhydroxyalkanoate in Eschericha coli from a single carbon source Eschenlauer, Arthur C.; Stoup, Sandra K.; Srienc, Friedrich International Journal of Biological Macromolecules, Vol. 19, Issue 2 https://doi.org/10.1016/0141-8130(96)01114-2	journal	August 1996
Intracellular pH in singleSaccharomyces cerevisiae cells Hernlem, B. J.; Srienc, F. Biotechnology Techniques, Vol. 3, Issue 2 https://doi.org/10.1007/BF01875557	journal	March 1989
Assay of Poly-β-Hydroxybutyric acid Law, John H.; Slepecky, Ralph A. Journal of Bacteriology, Vol. 82, Issue 1 https://doi.org/10.1128/JB.82.1.33-36.1961	journal	January 1961

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Related Subjects

modified
pseudomonas
oleovorans
phac1
nucleic
acids
encoding
bispecific
polyhydroxyalkanoate
polymerase
genetically
engineered
pha
tailored
substrate
specificity
provided
preferably
capable
copolymerizing
chain
length
monomer
medium
methods
disclosed
producing
assay
altered
pha polymerase
acids encoding
nucleic acid
nucleic acids
chain length
genetically engineered
pseudomonas oleovorans
oleovorans phac1
/800/435/999/

Title: Modified pseudomonas oleovorans phaC1 nucleic acids encoding bispecific polyhydroxyalkanoate polymerase

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References (36)

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