skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Microfabricated capillary array electrophoresis device and method

Abstract

A capillary array electrophoresis (CAE) micro-plate with an array of separation channels connected to an array of sample reservoirs on the plate. The sample reservoirs are organized into one or more sample injectors. One or more waste reservoirs are provided to collect wastes from reservoirs in each of the sample injectors. Additionally, a cathode reservoir is also multiplexed with one or more separation channels. To complete the electrical path, an anode reservoir which is common to some or all separation channels is also provided on the micro-plate. Moreover, the channel layout keeps the distance from the anode to each of the cathodes approximately constant.

Inventors:
 [1];  [2];  [3]
  1. Oakland, CA
  2. Moraga, CA
  3. Belmont, MA
Publication Date:
Research Org.:
Univ. of California (United States)
OSTI Identifier:
873356
Patent Number(s):
US 6143152
Assignee:
Regents of University of California (Berkeley, CA)
DOE Contract Number:  
FG03-91ER61125
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
microfabricated; capillary; array; electrophoresis; device; method; cae; micro-plate; separation; channels; connected; sample; reservoirs; plate; organized; injectors; waste; provided; collect; wastes; additionally; cathode; reservoir; multiplexed; complete; electrical; path; anode; common; moreover; channel; layout; distance; cathodes; approximately; constant; separation channel; capillary array; channels connected; microfabricated capillary; electrical path; sample injector; sample reservoirs; electrophoresis device; array electrophoresis; /204/

Citation Formats

Simpson, Peter C, Mathies, Richard A, and Woolley, Adam T. Microfabricated capillary array electrophoresis device and method. United States: N. p., 2000. Web.
Simpson, Peter C, Mathies, Richard A, & Woolley, Adam T. Microfabricated capillary array electrophoresis device and method. United States.
Simpson, Peter C, Mathies, Richard A, and Woolley, Adam T. Sat . "Microfabricated capillary array electrophoresis device and method". United States. https://www.osti.gov/servlets/purl/873356.
@article{osti_873356,
title = {Microfabricated capillary array electrophoresis device and method},
author = {Simpson, Peter C and Mathies, Richard A and Woolley, Adam T},
abstractNote = {A capillary array electrophoresis (CAE) micro-plate with an array of separation channels connected to an array of sample reservoirs on the plate. The sample reservoirs are organized into one or more sample injectors. One or more waste reservoirs are provided to collect wastes from reservoirs in each of the sample injectors. Additionally, a cathode reservoir is also multiplexed with one or more separation channels. To complete the electrical path, an anode reservoir which is common to some or all separation channels is also provided on the micro-plate. Moreover, the channel layout keeps the distance from the anode to each of the cathodes approximately constant.},
doi = {},
url = {https://www.osti.gov/biblio/873356}, journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {1}
}

Patent:

Save / Share:

Works referenced in this record:

Fluorescence scanner employing a macro scanning objective
conference, March 1996


Fluorescence scanner employing a macro scanning objective
conference, March 1996


High-throughput genetic analysis using microfabricated 96-sample capillary array electrophoresis microplates
journal, March 1998


High-throughput genetic analysis using microfabricated 96-sample capillary array electrophoresis microplates
journal, March 1998


High-speed separations of DNA sequencing reactions by capillary electrophoresis
journal, May 1990


High-speed separations of DNA sequencing reactions by capillary electrophoresis
journal, May 1990


A novel MHC class I–like gene is mutated in patients with hereditary haemochromatosis
journal, August 1996


A novel MHC class I–like gene is mutated in patients with hereditary haemochromatosis
journal, August 1996


Energy-transfer fluorescent reagents for DNA analyses
journal, February 1997


Energy-transfer fluorescent reagents for DNA analyses
journal, February 1997


High-performance electrophoresis
journal, January 1985


High-performance electrophoresis
journal, January 1985


Ultra-High-Speed DNA Sequencing Using Capillary Electrophoresis Chips
journal, October 1995


Ultra-High-Speed DNA Sequencing Using Capillary Electrophoresis Chips
journal, October 1995


Functional Integration of PCR Amplification and Capillary Electrophoresis in a Microfabricated DNA Analysis Device
journal, January 1996


Functional Integration of PCR Amplification and Capillary Electrophoresis in a Microfabricated DNA Analysis Device
journal, January 1996


Multiple Sheath-Flow Gel Capillary-Array Electrophoresis for Multicolor Fluorescent DNA Detection
journal, April 1994


Multiple Sheath-Flow Gel Capillary-Array Electrophoresis for Multicolor Fluorescent DNA Detection
journal, April 1994


Capillary electrophoresis and sample injection systems integrated on a planar glass chip
journal, September 1992


Capillary electrophoresis and sample injection systems integrated on a planar glass chip
journal, September 1992


Separation and analysis of DNA sequence reaction products by capillary gel electrophoresis
journal, September 1990


Separation and analysis of DNA sequence reaction products by capillary gel electrophoresis
journal, September 1990


Accessing Genetic Information with High-Density DNA Arrays
journal, October 1996


Accessing Genetic Information with High-Density DNA Arrays
journal, October 1996


Microsatellite-based cancer detection using capillary array electrophoresis and energy-transfer fluorescent primers
journal, January 1997


Microsatellite-based cancer detection using capillary array electrophoresis and energy-transfer fluorescent primers
journal, January 1997


Glass chips for high-speed capillary electrophoresis separations with submicrometer plate heights
journal, October 1993


Glass chips for high-speed capillary electrophoresis separations with submicrometer plate heights
journal, October 1993


High-Speed Separation of Antisense Oligonucleotides on a Micromachined Capillary Electrophoresis Device
journal, September 1994


High-Speed Separation of Antisense Oligonucleotides on a Micromachined Capillary Electrophoresis Device
journal, September 1994


Ultra-high-speed DNA fragment separations using microfabricated capillary array electrophoresis chips.
journal, November 1994


Ultra-high-speed DNA fragment separations using microfabricated capillary array electrophoresis chips.
journal, November 1994


Transport, Manipulation, and Reaction of Biological Cells On-Chip Using Electrokinetic Effects
journal, April 1997


Transport, Manipulation, and Reaction of Biological Cells On-Chip Using Electrokinetic Effects
journal, April 1997


DNA sequencing using capillary array electrophoresis
journal, September 1992


DNA sequencing using capillary array electrophoresis
journal, September 1992


A simple salting out procedure for extracting DNA from human nucleated cells
journal, January 1988


A simple salting out procedure for extracting DNA from human nucleated cells
journal, January 1988


High Speed Automated DNA Sequencing in Ultrathin Slab Gels
journal, January 1992


High Speed Automated DNA Sequencing in Ultrathin Slab Gels
journal, January 1992


DNA sequencing using a four-color confocal fluorescence capillary array scanner
journal, January 1996


DNA sequencing using a four-color confocal fluorescence capillary array scanner
journal, January 1996


Capillary array electrophoresis using laser-excited confocal fluorescence detection
journal, April 1992


Capillary array electrophoresis using laser-excited confocal fluorescence detection
journal, April 1992


Microchip Capillary Electrophoresis with an Integrated Postcolumn Reactor
journal, October 1994


Microchip Capillary Electrophoresis with an Integrated Postcolumn Reactor
journal, October 1994


Integrated Microdevice for DNA Restriction Fragment Analysis
journal, January 1996


Integrated Microdevice for DNA Restriction Fragment Analysis
journal, January 1996


Capillary gel electrophoresis for rapid, high resolution DNA sequencing
journal, January 1990


Capillary gel electrophoresis for rapid, high resolution DNA sequencing
journal, January 1990


High-Speed Parallel Separation of DNA Restriction Fragments Using Capillary Array Electrophoresis
journal, December 1993


High-Speed Parallel Separation of DNA Restriction Fragments Using Capillary Array Electrophoresis
journal, December 1993


Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a Chip
journal, August 1993


Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a Chip
journal, August 1993


Global prevalence of putative haemochromatosis mutations.
journal, April 1997


Global prevalence of putative haemochromatosis mutations.
journal, April 1997