skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Nonlinear vibrational microscopy

Abstract

The present invention is a method and apparatus for microscopic vibrational imaging using coherent Anti-Stokes Raman Scattering or Sum Frequency Generation. Microscopic imaging with a vibrational spectroscopic contrast is achieved by generating signals in a nonlinear optical process and spatially resolved detection of the signals. The spatial resolution is attained by minimizing the spot size of the optical interrogation beams on the sample. Minimizing the spot size relies upon a. directing at least two substantially co-axial laser beams (interrogation beams) through a microscope objective providing a focal spot on the sample; b. collecting a signal beam together with a residual beam from the at least two co-axial laser beams after passing through the sample; c. removing the residual beam; and d. detecting the signal beam thereby creating said pixel. The method has significantly higher spatial resolution then IR microscopy and higher sensitivity than spontaneous Raman microscopy with much lower average excitation powers. CARS and SFG microscopy does not rely on the presence of fluorophores, but retains the resolution and three-dimensional sectioning capability of confocal and two-photon fluorescence microscopy. Complementary to these techniques, CARS and SFG microscopy provides a contrast mechanism based on vibrational spectroscopy. This vibrational contrast mechanism, combined withmore » an unprecedented high sensitivity at a tolerable laser power level, provides a new approach for microscopic investigations of chemical and biological samples.« less

Inventors:
 [1];  [1];  [2]
  1. (Richland, WA)
  2. (Munchen, DE)
Publication Date:
Research Org.:
BATTELLE MEMORIAL INSTITUTE
OSTI Identifier:
873181
Patent Number(s):
US 6108081
Assignee:
Battelle Memorial Institute (Richland, WA) PNNL
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
nonlinear; vibrational; microscopy; method; apparatus; microscopic; imaging; coherent; anti-stokes; raman; scattering; frequency; generation; spectroscopic; contrast; achieved; generating; signals; optical; process; spatially; resolved; detection; spatial; resolution; attained; minimizing; spot; size; interrogation; beams; sample; relies; directing; substantially; co-axial; laser; microscope; objective; providing; focal; collecting; signal; beam; residual; passing; removing; detecting; creating; pixel; significantly; sensitivity; spontaneous; average; excitation; powers; cars; sfg; rely; presence; fluorophores; retains; three-dimensional; sectioning; capability; confocal; two-photon; fluorescence; complementary; techniques; provides; mechanism; based; spectroscopy; combined; unprecedented; tolerable; power; level; approach; investigations; chemical; biological; samples; biological samples; laser beams; power level; raman scattering; laser beam; spatial resolution; nonlinear optical; signal beam; focal spot; laser power; microscope objective; coherent anti-stokes; spot size; frequency generation; biological sample; nonlinear optic; optical interrogation; generating signals; optical process; anti-stokes raman; dual beam; /356/

Citation Formats

Holtom, Gary R., Xie, Xiaoliang Sunney, and Zumbusch, Andreas. Nonlinear vibrational microscopy. United States: N. p., 2000. Web.
Holtom, Gary R., Xie, Xiaoliang Sunney, & Zumbusch, Andreas. Nonlinear vibrational microscopy. United States.
Holtom, Gary R., Xie, Xiaoliang Sunney, and Zumbusch, Andreas. Sat . "Nonlinear vibrational microscopy". United States. https://www.osti.gov/servlets/purl/873181.
@article{osti_873181,
title = {Nonlinear vibrational microscopy},
author = {Holtom, Gary R. and Xie, Xiaoliang Sunney and Zumbusch, Andreas},
abstractNote = {The present invention is a method and apparatus for microscopic vibrational imaging using coherent Anti-Stokes Raman Scattering or Sum Frequency Generation. Microscopic imaging with a vibrational spectroscopic contrast is achieved by generating signals in a nonlinear optical process and spatially resolved detection of the signals. The spatial resolution is attained by minimizing the spot size of the optical interrogation beams on the sample. Minimizing the spot size relies upon a. directing at least two substantially co-axial laser beams (interrogation beams) through a microscope objective providing a focal spot on the sample; b. collecting a signal beam together with a residual beam from the at least two co-axial laser beams after passing through the sample; c. removing the residual beam; and d. detecting the signal beam thereby creating said pixel. The method has significantly higher spatial resolution then IR microscopy and higher sensitivity than spontaneous Raman microscopy with much lower average excitation powers. CARS and SFG microscopy does not rely on the presence of fluorophores, but retains the resolution and three-dimensional sectioning capability of confocal and two-photon fluorescence microscopy. Complementary to these techniques, CARS and SFG microscopy provides a contrast mechanism based on vibrational spectroscopy. This vibrational contrast mechanism, combined with an unprecedented high sensitivity at a tolerable laser power level, provides a new approach for microscopic investigations of chemical and biological samples.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {1}
}

Patent:

Save / Share: