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Title: cDNA encoding a polypeptide including a hev ein sequence

Patent ·
OSTI ID:873070
 [1];  [2];  [3];  [4]
  1. Okemos, MI
  2. Dilbeek, BE
  3. Scarsdale, NY
  4. New York, NY
+ Show Author Affiliations

A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74-79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe. A fusion protein was produced in E. coli from the protein of the present invention and maltose binding protein produced by the E. coli.

Research Organization:
Michigan State Univ., East Lansing, MI (United States)
DOE Contract Number:
AC02-76ER01338
Assignee:
Board of Trustees operating Michigan State University (East Lansing, MI)
Patent Number(s):
US 6083687
Application Number:
07/888,367
OSTI ID:
873070
Country of Publication:
United States
Language:
English

References (36)

Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP journal January 1986
Comparison of some molecular, enzymatic and antifungal properties of chitinases from thorn-apple, tobacco and wheat journal November 1988
Primary structure of wheat germ agglutinin isolectin 2. Peptide order deduced from x-ray structure journal January 1984
Structure of a tobacco endochitinase gene: evidence that different chitinase genes can arise by transposition of sequences encoding a cysteine-rich domain journal March 1990
The gene for the precursor of plastocyanin from the cyanobacterium Anabaena sp. PCC 7937: isolation, sequence and regulation journal March 1989
Expression of rice lectin is governed by two temporally and spatially regulated mRNAs in developing embryos. journal May 1989
A homologue of the nuclear coded 49 kd subunit of bovine mitochondrial NADH-ubiquinone reductase is coded in chloroplast DNA. journal March 1989
Systemic accumulation of specific mRNAs in response to wounding in poplar trees. journal October 1989
Host-Pathogen Interactions: XVIII. ISOLATION AND BIOLOGICAL ACTIVITY OF GLYCINOL, A PTEROCARPAN PHYTOALEXIN SYNTHESIZED BY SOYBEANS journal August 1981
Ethylene Biosynthesis and its Regulation in Higher Plants journal June 1984
Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein journal July 1988
Ethylene-regulated gene expression: molecular cloning of the genes encoding an endochitinase from Phaseolus vulgaris. journal September 1986
Co- and post-translational processing of the hevein preproprotein of latex of the rubber tree (Hevea brasiliensis) journal August 1991
Cloning and Characterization of Root-Specific Barley Lectin journal September 1989
DNA sequencing with chain-terminating inhibitors journal December 1977
Plastid-localised seed acyl-carrier protein of Brassica napus is encoded by a distinct, nuclear multigene family journal June 1988
Regulation of wound ethylene synthesis in plants journal July 1980
A simple and very efficient method for generating cDNA libraries journal November 1983
[15] A rapid single-stranded cloning, sequencing, insertion, and deletion strategy book January 1987
Isolation of cDNA clones coding for spinach nitrite reductase: Complete sequence and nitrate induction journal April 1988
Sequence complexity of polyadenylated ribonucleic acid from soybean suspension culture cells journal June 1979
A rapid and sensitive assay for chitinase using tritiated chitin journal December 1977
Plant chitinases are potent inhibitors of fungal growth journal November 1986
Regulation of synthesis of proteinase inhibitors I and II mRNAs in leaves of wounded tomato plants journal November 1986
Chitinase cDNA Cloning and mRNA Induction by Fungal Elicitor, Wounding, and Infection journal January 1988
Cell-type-specific expression of a wheat-germ agglutinin gene in embryos and young seedlings of Triticum aestivum journal December 1988
Regulation of a plant pathogenesis-related enzyme: Inhibition of chitinase and chitinase mRNA accumulation in cultured tobacco tissues by auxin and cytokinin journal January 1987
A Chitin-Binding Lectin from Stinging Nettle Rhizomes with Antifungal Properties journal September 1989
Abscisic acid is involved in the wound-induced expression of the proteinase inhibitor II gene in potato and tomato journal December 1989
Extensive homologies between lectins from non-leguminous plants journal January 1986
Homology between chitinases that are induced by TMV infection of tobacco journal January 1987
Effect of Ethylene Action Inhibitors upon Wound-Induced Gene Expression in Tomato Pericarp journal September 1989
A nuclear gene encoding the beta subunit of the mitochondrial ATP synthase in Nicotiana plumbaginifolia. journal September 1985
Wound-induced accumulation of mRNA containing a hevein sequence in laticifers of rubber tree (Hevea brasiliensis). journal October 1990
Translational regulation of protein synthesis, in response to light, at a critical stage of volvox development journal June 1985
Plant defense genes are regulated by ethylene journal August 1987

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cdna
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clone
hev1
hevein
isolated
via
polymerase
chain
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pcr
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oligonucleotides
corresponding
regions
primers
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1018
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amino
acids
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residues
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amino-terminal
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identical
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poplar
carboxyl-terminal
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74-79
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wound-inducible
wounding
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abscisic
ethylene
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stems
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deduced amino
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chain reaction
binding protein
acid sequence
amino acid
polymerase chain
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dna encoding
cdna encoding
residues followed
protein produced
putative signal
wound-induced genes
wound-inducible genes
abscisic acid
carboxyl-terminal portion
carboxyl-terminal region
maltose binding
plant hormones
chitin-binding proteins
amino-terminal region
sequence contains
signal sequence
oligonucleotides corresponding
mixed oligonucleotides
via polymerase
encoding hevein
hevea brasiliensis
acid residues
acid residue
acid polypeptide
brasiliensis latex
latex cdna
isolated via
polypeptide including
fusion protein
hormones abscisic
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