Method and apparatus for staining immobilized nucleic acids

Ramsey, J Michael; Foote, Robert S; Jacobson, Stephen C

Title: Method and apparatus for staining immobilized nucleic acids

Patent · Sat Jan 01 00:00:00 EST 2000

OSTI ID:872976

Ramsey, J Michael ^[1]; Foote, Robert S ^[2]; Jacobson, Stephen C ^[1]

Knoxville, TN
Oak Ridge, TN

A method for staining immobilized nucleic acids includes the steps of affixing DNA probes to a solid substrate, moving target DNA material into proximity with the DNA probes, whereby the target DNA hybridized with specific ones of the DNA probes, and moving a fluorescent dye into proximity with the hybridized target DNA, whereby the fluorescent dye binds to the hybridized DNA to enable subsequent detection of fluorescence.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

DOE Contract Number:: AC05-84OR21400

Assignee:: Lockheed Martin Energy Research Corporation (Oak Ridge, TN)

Patent Number(s):: US 6056859

OSTI ID:: 872976

Country of Publication:: United States

Language:: English

References (7)

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Manipulation and flow of biological fluids in straight channels micromachined in silicon Wilding, P.; Pfahler, J.; Bau, H. H. Clinical Chemistry, Vol. 40, Issue 1 https://doi.org/10.1093/clinchem/40.1.43	journal	January 1994
PCR in a silicon microstructure Wilding, P.; Shoffner, M. A.; Kricka, L. J. Clinical Chemistry, Vol. 40, Issue 9 https://doi.org/10.1093/clinchem/40.9.1815	journal	September 1994
Glass chips for high-speed capillary electrophoresis separations with submicrometer plate heights Effenhauser, Carlo S.; Manz, Andreas.; Widmer, H. Michael. Analytical Chemistry, Vol. 65, Issue 19 https://doi.org/10.1021/ac00067a015	journal	October 1993
Electric sample splitter for capillary zone electrophoresis Deml, M.; Foret, F.; Boček, P. Journal of Chromatography A, Vol. 320, Issue 1 https://doi.org/10.1016/S0021-9673(01)90491-1	journal	February 1985
Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a Chip Harrison, D. J.; Fluri, K.; Seiler, K. Science, Vol. 261, Issue 5123 https://doi.org/10.1126/science.261.5123.895	journal	August 1993
Capillary electrophoresis and sample injection systems integrated on a planar glass chip Harrison, D. Jed.; Manz, Andreas.; Fan, Zhonghui. Analytical Chemistry, Vol. 64, Issue 17 https://doi.org/10.1021/ac00041a030	journal	September 1992

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Related Subjects

method
apparatus
staining
immobilized
nucleic
acids
steps
affixing
dna
probes
solid
substrate
moving
target
material
proximity
whereby
hybridized
specific
ones
fluorescent
dye
binds
enable
subsequent
detection
fluorescence
hybridized dna
dna probes
hybridized target
nucleic acid
nucleic acids
fluorescent dye
target dna
solid substrate
subsequent detection
staining immobilized
dna probe
immobilized nucleic
/204/422/435/

Title: Method and apparatus for staining immobilized nucleic acids

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References (7)

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