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Title: Detection and isolation of nucleic acid sequences using a bifunctional hybridization probe

Abstract

A method for detecting and isolating a target sequence in a sample of nucleic acids is provided using a bifunctional hybridization probe capable of hybridizing to the target sequence that includes a detectable marker and a first complexing agent capable of forming a binding pair with a second complexing agent. A kit is also provided for detecting a target sequence in a sample of nucleic acids using a bifunctional hybridization probe according to this method.

Inventors:
 [1];  [2];  [3]
  1. San Ramon, CA
  2. Tracy, CA
  3. Walnut Creek, CA
Publication Date:
Research Org.:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
OSTI Identifier:
872867
Patent Number(s):
US 6027879
Assignee:
Regents of University of California (Oakland, CA)
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
detection; isolation; nucleic; acid; sequences; bifunctional; hybridization; probe; method; detecting; isolating; target; sequence; sample; acids; provided; capable; hybridizing; detectable; marker; complexing; agent; forming; binding; pair; kit; according; detectable marker; acid sequence; nucleic acid; acid sequences; nucleic acids; target sequence; complexing agent; hybridization probe; agent capable; bifunctional hybridization; probe capable; binding pair; /435/999/

Citation Formats

Lucas, Joe N, Straume, Tore, and Bogen, Kenneth T. Detection and isolation of nucleic acid sequences using a bifunctional hybridization probe. United States: N. p., 2000. Web.
Lucas, Joe N, Straume, Tore, & Bogen, Kenneth T. Detection and isolation of nucleic acid sequences using a bifunctional hybridization probe. United States.
Lucas, Joe N, Straume, Tore, and Bogen, Kenneth T. 2000. "Detection and isolation of nucleic acid sequences using a bifunctional hybridization probe". United States. https://www.osti.gov/servlets/purl/872867.
@article{osti_872867,
title = {Detection and isolation of nucleic acid sequences using a bifunctional hybridization probe},
author = {Lucas, Joe N and Straume, Tore and Bogen, Kenneth T},
abstractNote = {A method for detecting and isolating a target sequence in a sample of nucleic acids is provided using a bifunctional hybridization probe capable of hybridizing to the target sequence that includes a detectable marker and a first complexing agent capable of forming a binding pair with a second complexing agent. A kit is also provided for detecting a target sequence in a sample of nucleic acids using a bifunctional hybridization probe according to this method.},
doi = {},
url = {https://www.osti.gov/biblio/872867}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2000},
month = {Sat Jan 01 00:00:00 EST 2000}
}

Works referenced in this record:

Ligation of oligonucleotides to nucleic acids or proteins via disulfide bonds
journal, May 1988


Gene mapping and gene enrichment by the avidin-biotin interaction: use of cytochrome-c as a polyamine bridge
journal, January 1978


Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.
journal, November 1981