Detection and isolation of nucleic acid sequences using a bifunctional hybridization probe
Abstract
A method for detecting and isolating a target sequence in a sample of nucleic acids is provided using a bifunctional hybridization probe capable of hybridizing to the target sequence that includes a detectable marker and a first complexing agent capable of forming a binding pair with a second complexing agent. A kit is also provided for detecting a target sequence in a sample of nucleic acids using a bifunctional hybridization probe according to this method.
- Inventors:
-
- San Ramon, CA
- Tracy, CA
- Walnut Creek, CA
- Publication Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- OSTI Identifier:
- 872867
- Patent Number(s):
- US 6027879
- Assignee:
- Regents of University of California (Oakland, CA)
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- detection; isolation; nucleic; acid; sequences; bifunctional; hybridization; probe; method; detecting; isolating; target; sequence; sample; acids; provided; capable; hybridizing; detectable; marker; complexing; agent; forming; binding; pair; kit; according; detectable marker; acid sequence; nucleic acid; acid sequences; nucleic acids; target sequence; complexing agent; hybridization probe; agent capable; bifunctional hybridization; probe capable; binding pair; /435/999/
Citation Formats
Lucas, Joe N, Straume, Tore, and Bogen, Kenneth T. Detection and isolation of nucleic acid sequences using a bifunctional hybridization probe. United States: N. p., 2000.
Web.
Lucas, Joe N, Straume, Tore, & Bogen, Kenneth T. Detection and isolation of nucleic acid sequences using a bifunctional hybridization probe. United States.
Lucas, Joe N, Straume, Tore, and Bogen, Kenneth T. 2000.
"Detection and isolation of nucleic acid sequences using a bifunctional hybridization probe". United States. https://www.osti.gov/servlets/purl/872867.
@article{osti_872867,
title = {Detection and isolation of nucleic acid sequences using a bifunctional hybridization probe},
author = {Lucas, Joe N and Straume, Tore and Bogen, Kenneth T},
abstractNote = {A method for detecting and isolating a target sequence in a sample of nucleic acids is provided using a bifunctional hybridization probe capable of hybridizing to the target sequence that includes a detectable marker and a first complexing agent capable of forming a binding pair with a second complexing agent. A kit is also provided for detecting a target sequence in a sample of nucleic acids using a bifunctional hybridization probe according to this method.},
doi = {},
url = {https://www.osti.gov/biblio/872867},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2000},
month = {Sat Jan 01 00:00:00 EST 2000}
}
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