skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Nucleic acid indexing

Abstract

A restriction site indexing method for selectively amplifying any fragment generated by a Class II restriction enzyme includes adaptors specific to fragment ends containing adaptor indexing sequences complementary to fragment indexing sequences near the termini of fragments generated by Class II enzyme cleavage. A method for combinatorial indexing facilitates amplification of restriction fragments whose sequence is not known.

Inventors:
 [1];  [2]
  1. (Madison, WI)
  2. (Bellevue, WA)
Publication Date:
Research Org.:
University of Wisconsin
OSTI Identifier:
872707
Patent Number(s):
US 5994068
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI) CHO
DOE Contract Number:
FG02-91ER61122
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
nucleic; acid; indexing; restriction; site; method; selectively; amplifying; fragment; generated; ii; enzyme; adaptors; specific; containing; adaptor; sequences; complementary; near; termini; fragments; cleavage; combinatorial; facilitates; amplification; sequence; nucleic acid; restriction enzyme; restriction fragments; restriction site; indexing sequences; selectively amplifying; sequences complementary; site indexing; fragments generated; fragment generated; fragment indexing; indexing method; ii restriction; restriction fragment; acid indexing; adaptors specific; adaptor indexing; containing adaptor; /435/999/

Citation Formats

Guilfoyle, Richard A., and Guo, Zhen. Nucleic acid indexing. United States: N. p., 1999. Web.
Guilfoyle, Richard A., & Guo, Zhen. Nucleic acid indexing. United States.
Guilfoyle, Richard A., and Guo, Zhen. Fri . "Nucleic acid indexing". United States. doi:. https://www.osti.gov/servlets/purl/872707.
@article{osti_872707,
title = {Nucleic acid indexing},
author = {Guilfoyle, Richard A. and Guo, Zhen},
abstractNote = {A restriction site indexing method for selectively amplifying any fragment generated by a Class II restriction enzyme includes adaptors specific to fragment ends containing adaptor indexing sequences complementary to fragment indexing sequences near the termini of fragments generated by Class II enzyme cleavage. A method for combinatorial indexing facilitates amplification of restriction fragments whose sequence is not known.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1999},
month = {Fri Jan 01 00:00:00 EST 1999}
}

Patent:

Save / Share: