Invasive cleavage of nucleic acids

Prudent, James R; Hall, Jeff G; Lyamichev, Victor I; Brow, Mary Ann D; Dahlberg, James E

Title: Invasive cleavage of nucleic acids

Patent · Fri Jan 01 00:00:00 EST 1999

OSTI ID:872670

Prudent, James R ^[1]; Hall, Jeff G ^[1]; Lyamichev, Victor I ^[1]; Brow, Mary Ann D ^[1]; Dahlberg, James E ^[1]

Madison, WI

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

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Research Organization:: Third Wave Technologies, Inc., Madison, WI (United States)

DOE Contract Number:: FG02-94ER81891

Assignee:: Third Wave Technologies, Inc. (WI)

Patent Number(s):: US 5985557

OSTI ID:: 872670

Country of Publication:: United States

Language:: English

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target-dependent cleavage
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Title: Invasive cleavage of nucleic acids

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