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Title: Invasive cleavage of nucleic acids

Abstract

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

Inventors:
 [1];  [1];  [1];  [1];  [1]
  1. Madison, WI
Publication Date:
Research Org.:
Third Wave Technologies, Inc., Madison, WI (United States)
OSTI Identifier:
872670
Patent Number(s):
US 5985557
Assignee:
Third Wave Technologies, Inc. (WI)
DOE Contract Number:  
FG02-94ER81891
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
invasive; cleavage; nucleic; acids; relates; means; detection; characterization; acid; sequences; variations; methods; forming; structure; target; sequence; cleaving; site-specific; manner; structure-specific; nuclease; activity; variety; enzymes; cleave; target-dependent; indicating; presence; specific; cleavage structure; acid sequence; nucleic acid; acid sequences; nucleic acids; target sequence; acid cleavage; specific nucleic; structure-specific nuclease; specific variations; site-specific manner; nuclease activity; target-dependent cleavage; specific manner; /435/999/

Citation Formats

Prudent, James R, Hall, Jeff G, Lyamichev, Victor I, Brow, Mary Ann D, and Dahlberg, James E. Invasive cleavage of nucleic acids. United States: N. p., 1999. Web.
Prudent, James R, Hall, Jeff G, Lyamichev, Victor I, Brow, Mary Ann D, & Dahlberg, James E. Invasive cleavage of nucleic acids. United States.
Prudent, James R, Hall, Jeff G, Lyamichev, Victor I, Brow, Mary Ann D, and Dahlberg, James E. Fri . "Invasive cleavage of nucleic acids". United States. https://www.osti.gov/servlets/purl/872670.
@article{osti_872670,
title = {Invasive cleavage of nucleic acids},
author = {Prudent, James R and Hall, Jeff G and Lyamichev, Victor I and Brow, Mary Ann D and Dahlberg, James E},
abstractNote = {The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}

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