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Title: Self-assembling multimeric nucleic acid constructs

Abstract

The invention is directed to constructs and compositions containing multimeric forms of nucleic acid. Multimeric nucleic acids comprise single-stranded nucleic acids attached via biotin to streptavidin and bound with a functional group. These constructs can be utilized in vivo to treat or identify diseased tissue or cells. Repeated administrations of multimeric nucleic acid compositions produce a rapid and specific amplification of nucleic acid constructs and their attached functional groups. For treatment purposes, functional groups may be toxins, radioisotopes, genes or enzymes. Diagnostically, labeled multimeric constructs may be used to identify specific targets in vivo or in vitro. Multimeric nucleic acids may also be used in nanotechnology and to create self-assembling polymeric aggregates such as membranes of defined porosity, microcircuits and many other products.

Inventors:
 [1];  [2];  [1];  [1];  [3];  [4]
  1. Boston, MA
  2. Bremen, DE
  3. Brookline, MA
  4. Southborough, MA
Publication Date:
Research Org.:
Boston University
OSTI Identifier:
872575
Patent Number(s):
US 5965133
Application Number:
08/967297
Assignee:
Trustees of Boston University (Boston, MA); Univ. of Mass. Medical Center (Worcester, MA)
DOE Contract Number:  
FG02-93ER61656
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
self-assembling; multimeric; nucleic; acid; constructs; directed; compositions; containing; forms; acids; comprise; single-stranded; attached; via; biotin; streptavidin; bound; functional; utilized; vivo; treat; identify; diseased; tissue; cells; repeated; administrations; produce; rapid; specific; amplification; treatment; purposes; toxins; radioisotopes; genes; enzymes; diagnostically; labeled; targets; vitro; nanotechnology; create; polymeric; aggregates; membranes; defined; porosity; microcircuits; products; nucleic acid; nucleic acids; compositions containing; single-stranded nucleic; multimeric nucleic; specific target; identify specific; attached via; acid constructs; self-assembling multimeric; compositions contain; compositions produce; comprise single-stranded; multimeric forms; diseased tissue; via biotin; stranded nucleic; acids attached; acids comprise; containing multimeric; /424/514/530/999/

Citation Formats

Cantor, Charles R, Niemeyer, Christof M, Smith, Cassandra L, Sano, Takeshi, Hnatowich, Donald J, and Rusckowski, Mary. Self-assembling multimeric nucleic acid constructs. United States: N. p., 1999. Web.
Cantor, Charles R, Niemeyer, Christof M, Smith, Cassandra L, Sano, Takeshi, Hnatowich, Donald J, & Rusckowski, Mary. Self-assembling multimeric nucleic acid constructs. United States.
Cantor, Charles R, Niemeyer, Christof M, Smith, Cassandra L, Sano, Takeshi, Hnatowich, Donald J, and Rusckowski, Mary. 1999. "Self-assembling multimeric nucleic acid constructs". United States. https://www.osti.gov/servlets/purl/872575.
@article{osti_872575,
title = {Self-assembling multimeric nucleic acid constructs},
author = {Cantor, Charles R and Niemeyer, Christof M and Smith, Cassandra L and Sano, Takeshi and Hnatowich, Donald J and Rusckowski, Mary},
abstractNote = {The invention is directed to constructs and compositions containing multimeric forms of nucleic acid. Multimeric nucleic acids comprise single-stranded nucleic acids attached via biotin to streptavidin and bound with a functional group. These constructs can be utilized in vivo to treat or identify diseased tissue or cells. Repeated administrations of multimeric nucleic acid compositions produce a rapid and specific amplification of nucleic acid constructs and their attached functional groups. For treatment purposes, functional groups may be toxins, radioisotopes, genes or enzymes. Diagnostically, labeled multimeric constructs may be used to identify specific targets in vivo or in vitro. Multimeric nucleic acids may also be used in nanotechnology and to create self-assembling polymeric aggregates such as membranes of defined porosity, microcircuits and many other products.},
doi = {},
url = {https://www.osti.gov/biblio/872575}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Oct 12 00:00:00 EDT 1999},
month = {Tue Oct 12 00:00:00 EDT 1999}
}

Works referenced in this record:

Amplifying DNA Probe Signals: A ‘christmas Tree’ Approach
journal, October 1988


Affinity capture electrophoresis for sequence-specific DNA purification
journal, June 1992


Immuno-PCR with a commercially available avidin system
journal, April 1993


Atomic force microscopy of biochemically tagged DNA.
journal, May 1993


Sequence-specific DNA purification by triplex affinity capture.
journal, January 1992


[66] Biotinylated nucleotides for labeling and detecting DNA
book, January 1990