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Title: Isolated spinach ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit .sup..epsilon. N-methyltransferase and method of inactivating ribulose-1,5-bisphosphatase carboxylase/oxygenase large subunit .sup..epsilon. N-methyltransferase activity

Patent ·
OSTI ID:872316
 [1]
  1. Lexington, KY
+ Show Author Affiliations

The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) .sup..epsilon. N-methyltransferase (protein methylase III or Rubisco LSMT) from a plant which has a des(methyl) lysyl residue in the LS is disclosed. In addition, the full-length cDNA clones for Rubisco LSMT are disclosed. Transgenic plants and methods of producing same which have the Rubisco LSMT gene inserted into the DNA are also provided. Further, methods of inactivating the enzymatic activity of Rubisco LSMT are also disclosed.

Research Organization:
University of Kentucky Research Foundation (Lexington, KY)
DOE Contract Number:
FG05-92ER26075
Assignee:
University of Kentucky Research Foundation (Lexington, KY)
Patent Number(s):
US 5908972
OSTI ID:
872316
Country of Publication:
United States
Language:
English

References (12)

Affinity Purification of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Large Subunit ϵN-Methyltransferase journal August 1995
Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes journal August 1988
Post-translational modifications in the large subunit of ribulose bisphosphate carboxylase/oxygenase. journal March 1989
Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit N-methyltransferase journal January 1995
Light-regulated methylation of chloroplast proteins. journal July 1987
Isolation and characterization of a light-inducible, organ-specific gene from potato and analysis of its expression after tagging and transfer into tobacco and potato shoots journal October 1986
Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans. journal April 1990
Posttranslational Modifications in the Amino- Terminal Region of the Large Subunit of Ribulose- 1,5-Bisphosphate Carboxylase/Oxygenase from Several Plant Species journal March 1992
Cloning and developmental expression of the sucrose-phosphate-synthase gene from spinach journal June 1993
Partial Purification and Characterization of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Large Subunit ε N -Methyltransferase journal November 1991
Reaction-intermediate analogue binding by ribulose bisphosphate carboxylase/oxygenase causes specific changes in proteolytic sensitivity: the amino-terminal residue of the large subunit is acetylated proline. journal March 1988
Protein Methylation in Pea Chloroplasts journal July 1990

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isolated
spinach
ribulose-1
5-bisphosphate
carboxylase
oxygenase
subunit
epsilon
n-methyltransferase
method
inactivating
5-bisphosphatase
activity
sequence
rubisco
protein
methylase
iii
lsmt
plant
methyl
lysyl
residue
disclosed
addition
full-length
cdna
clones
transgenic
plants
methods
producing
inserted
dna
provided
enzymatic
cdna clones
cdna clone
rubisco lsmt
transgenic plants
protein methylase
methylase iii
full-length cdna
n-methyltransferase activity
enzymatic activity
transgenic plant
spinach ribulose-1
inactivating ribulose-1
isolated spinach
/800/435/999/