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Title: Monoterpene synthases from common sage (Salvia officinalis)

Abstract

cDNAs encoding (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase from common sage (Salvia officinalis) have been isolated and sequenced, and the corresponding amino acid sequences has been determined. Accordingly, isolated DNA sequences (SEQ ID No:1; SEQ ID No:3 and SEQ ID No:5) are provided which code for the expression of (+)-bornyl diphosphate synthase (SEQ ID No:2), 1,8-cineole synthase (SEQ ID No:4) and (+)-sabinene synthase SEQ ID No:6), respectively, from sage (Salvia officinalis). In other aspects, replicable recombinant cloning vehicles are provided which code for (+)-bornyl diphosphate synthase, 1,8-cineole synthase or (+)-sabinene synthase, or for a base sequence sufficiently complementary to at least a portion of (+)-bornyl diphosphate synthase, 1,8-cineole synthase or (+)-sabinene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (+)-bornyl diphosphate synthase, 1,8-cineole synthase or (+)-sabinene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant monoterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts. Recombinant (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase may be used to obtain expressionmore » or enhanced expression of (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase in plants in order to enhance the production of monoterpenoids, or may be otherwise employed for the regulation or expression of (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase, or the production of their products.« less

Inventors:
 [1];  [1];  [1];  [2]
+ Show Inventor Affiliations
  1. Pullman, WA
  2. Christchurch 5, NZ
Publication Date:
Research Org.:
Washington State University Research Foundation (Pullman, WA)
OSTI Identifier:
872239
Patent Number(s):
US 5891697
Assignee:
Washington State University Research Foundation (Pullman, WA)
DOE Contract Number:  
FG03-96ER20212
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
monoterpene; synthases; common; sage; salvia; officinalis; cdnas; encoding; -bornyl; diphosphate; synthase; 8-cineole; -sabinene; isolated; sequenced; corresponding; amino; acid; sequences; determined; accordingly; dna; seq; provided; code; expression; respectively; aspects; replicable; recombinant; cloning; vehicles; base; sequence; sufficiently; complementary; portion; rna; enable; hybridization; therewith; modified; host; cells; transformed; transfected; infected; injected; vehicle; systems; methods; aforementioned; facilitate; production; isolation; purification; significant; amounts; obtain; enhanced; plants; enhance; monoterpenoids; otherwise; employed; regulation; products; sequence sufficiently; modified host; cloning vehicles; recombinant expression; replicable recombinant; obtain expression; otherwise employed; enable hybridization; hybridization therewith; synthase dna; recombinant cloning; dna sequences; dna sequence; isolated dna; significant amounts; acid sequence; amino acid; acid sequences; diphosphate synthase; host cells; sequence encoding; base sequence; cloning vehicle; host cell; sufficiently complementary; salvia officinalis; cdnas encoding; significant amount; corresponding amino; enhanced expression; aforementioned recombinant; common sage; /435/530/999/

Citation Formats

Croteau, Rodney Bruce, Wise, Mitchell Lynn, Katahira, Eva Joy, and Savage, Thomas Jonathan. Monoterpene synthases from common sage (Salvia officinalis). United States: N. p., 1999. Web.
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Croteau, Rodney Bruce, Wise, Mitchell Lynn, Katahira, Eva Joy, & Savage, Thomas Jonathan. Monoterpene synthases from common sage (Salvia officinalis). United States.
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Croteau, Rodney Bruce, Wise, Mitchell Lynn, Katahira, Eva Joy, and Savage, Thomas Jonathan. 1999. "Monoterpene synthases from common sage (Salvia officinalis)". United States. https://www.osti.gov/servlets/purl/872239.
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@article{osti_872239,
title = {Monoterpene synthases from common sage (Salvia officinalis)},
author = {Croteau, Rodney Bruce and Wise, Mitchell Lynn and Katahira, Eva Joy and Savage, Thomas Jonathan},
abstractNote = {cDNAs encoding (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase from common sage (Salvia officinalis) have been isolated and sequenced, and the corresponding amino acid sequences has been determined. Accordingly, isolated DNA sequences (SEQ ID No:1; SEQ ID No:3 and SEQ ID No:5) are provided which code for the expression of (+)-bornyl diphosphate synthase (SEQ ID No:2), 1,8-cineole synthase (SEQ ID No:4) and (+)-sabinene synthase SEQ ID No:6), respectively, from sage (Salvia officinalis). In other aspects, replicable recombinant cloning vehicles are provided which code for (+)-bornyl diphosphate synthase, 1,8-cineole synthase or (+)-sabinene synthase, or for a base sequence sufficiently complementary to at least a portion of (+)-bornyl diphosphate synthase, 1,8-cineole synthase or (+)-sabinene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (+)-bornyl diphosphate synthase, 1,8-cineole synthase or (+)-sabinene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant monoterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts. Recombinant (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase may be used to obtain expression or enhanced expression of (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase in plants in order to enhance the production of monoterpenoids, or may be otherwise employed for the regulation or expression of (+)-bornyl diphosphate synthase, 1,8-cineole synthase and (+)-sabinene synthase, or the production of their products.},
doi = {},
url = {https://www.osti.gov/biblio/872239}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1999},
month = {Fri Jan 01 00:00:00 EST 1999}
}
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Works referenced in this record:

Isolation and Characterization of an Active-Site Peptide from a Monoterpene Cyclase Labeled with a Mechanism-Based Inhibitor
journal, February 1995

cDNA Cloning, Characterization, and Functional Expression of 4S-(−)-Limonene Synthase fromPerilla frutescens
journal, August 1996

Isotopically sensitive branching as a tool for evaluating multiple product formation by monoterpene cyclases
journal, July 1991

Monoterpene Biosynthesis
book, May 1992

Monoterpene Biosynthesis: Isotope Effects Associated with Bicyclic Olefin Formation Catalyzed by Pinene Synthases from Sage (Salvia officinalis)
journal, February 1994

Biosynthesis of Monoterpenes: Partial Purification, Characterization, and Mechanism of Action of 1,8-Cineole Synthase
journal, February 1994

Stereochemistry of the Proton Elimination in the Formation of (+)- and (−)-α-Pinene by Monoterpene Cyclases from Sage (Salvia officinalis)
journal, February 1994

Induced oleoresin biosynthesis in grand fir as a defense against bark beetles.
journal, May 1995

Biosynthesis of Monoterpenes
journal, February 1989

Monoterpene Synthases from Grand Fir ( Abies grandis ) : cDNA ISOLATION, CHARACTERIZATION, AND FUNCTIONAL EXPRESSION OF MYRCENE SYNTHASE, (−)-(4
journal, August 1997

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