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Title: Array of nucleic acid probes on biological chips for diagnosis of HIV and methods of using the same

Abstract

The invention provides an array of oligonucleotide probes immobilized on a solid support for analysis of a target sequence from a human immunodeficiency virus. The array comprises at least four sets of oligonucleotide probes 9 to 21 nucleotides in length. A first probe set has a probe corresponding to each nucleotide in a reference sequence from a human immunodeficiency virus. A probe is related to its corresponding nucleotide by being exactly complementary to a subsequence of the reference sequence that includes the corresponding nucleotide. Thus, each probe has a position, designated an interrogation position, that is occupied by a complementary nucleotide to the corresponding nucleotide. The three additional probe sets each have a corresponding probe for each probe in the first probe set. Thus, for each nucleotide in the reference sequence, there are four corresponding probes, one from each of the probe sets. The three corresponding probes in the three additional probe sets are identical to the corresponding probe from the first probe or a subsequence thereof that includes the interrogation position, except that the interrogation position is occupied by a different nucleotide in each of the four corresponding probes.

Inventors:
 [1];  [2];  [1];  [3];  [4]
  1. Palo Alto, CA
  2. Santa Clara, CA
  3. Mountain View, CA
  4. San Jose, CA
Publication Date:
Research Org.:
Affymetrix, Inc. (Santa Clara, CA)
OSTI Identifier:
872103
Patent Number(s):
US 5861242
Application Number:
08/781,550
Assignee:
Affymetrix, Inc. (Santa Clara, CA)
DOE Contract Number:  
FG03-92ER81275; 1R01HG00813-01
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
array; nucleic; acid; probes; biological; chips; diagnosis; hiv; methods; provides; oligonucleotide; immobilized; solid; support; analysis; target; sequence; human; immunodeficiency; virus; comprises; sets; 21; nucleotides; length; probe; set; corresponding; nucleotide; reference; related; exactly; complementary; subsequence; position; designated; interrogation; occupied; additional; identical; oligonucleotide probes; reference sequence; solid support; acid probes; nucleic acid; target sequence; acid probe; array comprises; complementary nucleotide; oligonucleotide probe; biological chips; /435/422/436/999/

Citation Formats

Chee, Mark, Gingeras, Thomas R, Fodor, Stephen P. A., Hubble, Earl A, and Morris, MacDonald S. Array of nucleic acid probes on biological chips for diagnosis of HIV and methods of using the same. United States: N. p., 1999. Web.
Chee, Mark, Gingeras, Thomas R, Fodor, Stephen P. A., Hubble, Earl A, & Morris, MacDonald S. Array of nucleic acid probes on biological chips for diagnosis of HIV and methods of using the same. United States.
Chee, Mark, Gingeras, Thomas R, Fodor, Stephen P. A., Hubble, Earl A, and Morris, MacDonald S. Tue . "Array of nucleic acid probes on biological chips for diagnosis of HIV and methods of using the same". United States. https://www.osti.gov/servlets/purl/872103.
@article{osti_872103,
title = {Array of nucleic acid probes on biological chips for diagnosis of HIV and methods of using the same},
author = {Chee, Mark and Gingeras, Thomas R and Fodor, Stephen P. A. and Hubble, Earl A and Morris, MacDonald S},
abstractNote = {The invention provides an array of oligonucleotide probes immobilized on a solid support for analysis of a target sequence from a human immunodeficiency virus. The array comprises at least four sets of oligonucleotide probes 9 to 21 nucleotides in length. A first probe set has a probe corresponding to each nucleotide in a reference sequence from a human immunodeficiency virus. A probe is related to its corresponding nucleotide by being exactly complementary to a subsequence of the reference sequence that includes the corresponding nucleotide. Thus, each probe has a position, designated an interrogation position, that is occupied by a complementary nucleotide to the corresponding nucleotide. The three additional probe sets each have a corresponding probe for each probe in the first probe set. Thus, for each nucleotide in the reference sequence, there are four corresponding probes, one from each of the probe sets. The three corresponding probes in the three additional probe sets are identical to the corresponding probe from the first probe or a subsequence thereof that includes the interrogation position, except that the interrogation position is occupied by a different nucleotide in each of the four corresponding probes.},
doi = {},
url = {https://www.osti.gov/biblio/872103}, journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}

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