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Title: Methods of detection using a cellulose binding domain fusion product

Patent ·
OSTI ID:872077
 [1];  [2];  [3];  [3]
  1. Shimshon, IL
  2. North Gallilea, IL
  3. Davis, CA
+ Show Author Affiliations

A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.

Research Organization:
Univ. of California (United States)
DOE Contract Number:
FG03-92ER20069
Assignee:
Yissum Research Development Company of Hebrew University of Jerusalem (IL); University of California (CA)
Patent Number(s):
US 5856201
OSTI ID:
872077
Country of Publication:
United States
Language:
English

References (35)

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Fusion to an endoglucanase allows alkaline phosphatase to bind to cellulose journal February 1989
celA from Bacillus lautus PL236 encodes a novel cellulose-binding endo-beta-1,4-glucanase. journal January 1992
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Gene sequence and properties of Cell, a family E endoglucanase from Clostridium thermocellum journal February 1993
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Nucleotide sequence of Clostridium cellulovorans gene homologous to cyclic-AMP dependent kinase journal January 1991
Chitinase from Serratia marcescens book January 1988
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Immobilized endo-.beta.-glucosidase enriches flavor of wine and passion fruit juice journal June 1990
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Homologous plant and bacterial proteins chaperone oligomeric protein assembly journal May 1988
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Primary sequence analysis of Clostridium cellulovorans cellulose binding protein A. journal April 1992
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A mycobacterial 65-kD heat shock protein induces antigen-specific suppression of adjuvant arthritis, but is not itself arthritogenic. journal January 1990
Synovial fluid-derivedYersinia-reactive T cells responding to human 65-kDa heat-shock protein and heat-stressed antigen-presenting cells journal September 1991
Cloning of the mycobacterial epitope recognized by T lymphocytes in adjuvant arthritis journal January 1988
Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors. journal April 1985
Cellulose-binding domains: potential for purification of complex proteins journal January 1992
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Essential 170-kDa subunit for degradation of crystalline cellulose by Clostridium cellulovorans cellulase. journal March 1990
Nucleotide Sequences and Novel Structural Features of Human and Chinese Hamster hsp60 (Chaperonin) Gene Families journal October 1990
Sequence analysis of the Clostridium stercorarium celZ gene encoding a thermoactive cellulase (Avicelase I): Identification of catalytic and cellulose-binding domains journal September 1990
Heat-Shock Proteins as Immunogenic Bacterial Antigens with the Potential to Induce and Regulate Autoimmune Arthritis journal June 1991
The Mycobacterium tuberculosis 65-kilodalton antigen is a heat shock protein which corresponds to common antigen and to the Escherichia coli GroEL protein. journal January 1988
Structure of a Bacillus subtilis endo-β-l,4-glucanase gene journal January 1986
Cloning of Clostridiumcellulovoransendo-1,4-β-glucanase genes journal June 1990
Primary structure of a human mitochondrial protein homologous to the bacterial and plant chaperonins and to the 65-kilodalton mycobacterial antigen. journal May 1989
Deciphering the message in protein sequences: tolerance to amino acid substitutions journal March 1990
Induction and therapy of autoimmune diabetes in the non-obese diabetic (NOD/Lt) mouse by a 65-kDa heat shock protein. journal February 1990
Non–Hydrolytic Disruption of Cellulose Fibres by the Binding Domain of a Bacterial Cellulase journal November 1991
Molecular cloning of a Chinese hamster mitochondrial protein related to the “chaperonin” family of bacterial and plant proteins journal July 1989

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Related Subjects

methods
detection
cellulose
binding
domain
fusion
product
cbd
affinity
crystalline
chitin
disclosed
molecular
cloning
recombinant
production
products
comprising
protein
likewise
described
wide
range
applications
contemplated
including
drug
delivery
separations
diagnostic
techniques
molecular cloning
affinity separations
crystalline cellulose
including drug
likewise described
diagnostic techniques
binding domain
cellulose binding
wide range
products comprising
drug delivery
fusion products
recombinant production
domain fusion
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