Method for improved selectivity in photo-activation and detection of molecular diagnostic agents

Wachter, Eric A; Fisher, Walter G; Dees, H Craig

Title: Method for improved selectivity in photo-activation and detection of molecular diagnostic agents

Abstract

A method for the imaging of a particular volume of plant or animal tissue, wherein the plant or animal tissue contains at least one photo-active molecular agent. The method includes the steps of treating the particular volume of the plant or animal tissue with light sufficient to promote a simultaneous two-photon excitation of the photo-active molecular agent contained in the particular volume of the plant or animal tissue, photo-activating at least one of the at least one photo-active molecular agent in the particular volume of the plant or animal tissue, thereby producing at least one photo-activated molecular agent, wherein the at least one photo-activated molecular agent emits energy, detecting the energy emitted by the at least one photo-activated molecular agent, and producing a detected energy signal which is characteristic of the particular volume of plant or animal tissue. The present invention is also a method for the imaging of a particular volume of material, wherein the material contains at least one photo-active molecular agent.

Inventors:

Wachter, Eric A ^[1]; Fisher, Walter G ^[2]; Dees, H Craig ^[2]

Oak Ridge, TN
Knoxville, TN

Publication Date:: Thu Jan 01 00:00:00 EST 1998

Research Org.:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

OSTI Identifier:: 871959

Patent Number(s):: US 5832931

Assignee:: Photogen, Inc. (Oak Ridge, TN)

DOE Contract Number:: AC05-84OR21400

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: method; improved; selectivity; photo-activation; detection; molecular; diagnostic; agents; imaging; particular; volume; plant; animal; tissue; contains; photo-active; agent; steps; treating; light; sufficient; promote; simultaneous; two-photon; excitation; contained; photo-activating; producing; photo-activated; emits; energy; detecting; emitted; detected; signal; characteristic; material; two-photon excitation; photo-activated molecular; simultaneous two-photon; photo-active molecular; molecular agent; animal tissue; particular volume; improved selectivity; energy emitted; light sufficient; material contains; diagnostic agent; diagnostic agents; /128/600/

Citation Formats


                    Wachter, Eric A, Fisher, Walter G, and Dees, H Craig. Method for improved selectivity in photo-activation and detection of molecular diagnostic agents.  United States: N. p., 1998. 
        Web.

Copy to clipboard


                    Wachter, Eric A, Fisher, Walter G, & Dees, H Craig. Method for improved selectivity in photo-activation and detection of molecular diagnostic agents.  United States.

Copy to clipboard


                    Wachter, Eric A, Fisher, Walter G, and Dees, H Craig. 1998.  
        "Method for improved selectivity in photo-activation and detection of molecular diagnostic agents".  United States.  https://www.osti.gov/servlets/purl/871959.

Copy to clipboard


                    
@article{osti_871959,

  title        = {Method for improved selectivity in photo-activation and detection of molecular diagnostic agents},

  author       = {Wachter, Eric A and Fisher, Walter G and Dees, H Craig},

  abstractNote = {A method for the imaging of a particular volume of plant or animal tissue, wherein the plant or animal tissue contains at least one photo-active molecular agent. The method includes the steps of treating the particular volume of the plant or animal tissue with light sufficient to promote a simultaneous two-photon excitation of the photo-active molecular agent contained in the particular volume of the plant or animal tissue, photo-activating at least one of the at least one photo-active molecular agent in the particular volume of the plant or animal tissue, thereby producing at least one photo-activated molecular agent, wherein the at least one photo-activated molecular agent emits energy, detecting the energy emitted by the at least one photo-activated molecular agent, and producing a detected energy signal which is characteristic of the particular volume of plant or animal tissue. The present invention is also a method for the imaging of a particular volume of material, wherein the material contains at least one photo-active molecular agent.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/871959},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Thu Jan 01 00:00:00 EST 1998},

  month        = {Thu Jan 01 00:00:00 EST 1998}

}

Copy to clipboard

Patent:

View Patent

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

Two-step laser activation of hematoporphyrin derivative
journal, April 1982

Andreoni, A.; Cubeddu, R.; De Silvestri, S.
Chemical Physics Letters, Vol. 88, Issue 1
https://doi.org/10.1016/0009-2614(82)80065-1

Dispersion of the two-photon cross section in rhodamine dyes
journal, October 1972

Hermann, J. P.; Ducuing, J.
Optics Communications, Vol. 6, Issue 2
https://doi.org/10.1016/0030-4018(72)90204-0

A simple conceptual model for two‐photon absorption
journal, January 1986

Moscatelli, Frank A.
American Journal of Physics, Vol. 54, Issue 1
https://doi.org/10.1119/1.14770

Multiphoton Spectroscopy
journal, October 1967

Peticolas, W. L.
Annual Review of Physical Chemistry, Vol. 18, Issue 1
https://doi.org/10.1146/annurev.pc.18.100167.001313

Mechanistic investigation of doxycycline photosensitization by picosecond-pulsed and continuous wave laser irradiation of cells in culture.
journal, April 1990

Shea, C. R.; Hefetz, Y.; Gillies, R.
Journal of Biological Chemistry, Vol. 265, Issue 11
https://doi.org/10.1016/S0021-9258(19)39279-8

Über Elementarakte mit zwei Quantensprüngen
journal, January 1931

Göppert-Mayer, Maria
Annalen der Physik, Vol. 401, Issue 3
https://doi.org/10.1002/andp.19314010303

A study on the possible involvement of nonlinear mechanism of light absorption by HpD with Nd: YAG laser
journal, January 1986

Marchesini, Renato; Melloni, Elsa; Fava, Giannino
Lasers in Surgery and Medicine, Vol. 6, Issue 3
https://doi.org/10.1002/lsm.1900060306

Limitations Arising from Optical Saturation in Fluorescence and Thermal Lens Spectrometries Using Pulsed Laser Excitation: Application to the Determination of the Fluorescence Quantum Yield of Rhodamine 6G
journal, December 1996

Georges, J.; Arnaud, N.; Parise, L.
Applied Spectroscopy, Vol. 50, Issue 12
https://doi.org/10.1366/0003702963904511

Two Photons Shine In 3-D Data Storage: Organic dyes show promise for computer memories, cancer therapy, microscopy
journal, September 1996

Dagani, Ron
Chemical & Engineering News, Vol. 74, Issue 39
https://doi.org/10.1021/cen-v074n039.p068

Photodynamic Therapy Using Pheophorbide a and Nd:YAG Laser
journal, January 1991

Fujishima, Ichiro; Sakai, Tsuneo; Tanaka, Tokutaro
Neurologia medico-chirurgica, Vol. 31, Issue 5
https://doi.org/10.2176/nmc.31.257

Laser Fundamentals
book, January 1981

Lytle, Fred E.
Lasers in Chemical Analysis
https://doi.org/10.1007/978-1-4612-6009-7_1

Excited State Symmetry Assignment Through Polarized Two‐Photon Absorption Studies of Fluids
journal, September 1971

McClain, W. M.
The Journal of Chemical Physics, Vol. 55, Issue 6
https://doi.org/10.1063/1.1676494

Multiphoton Resonant Nonlinear Optical Processes in Organic Molecules
book, May 1996

Prasad, Paras N.; He, Guang S.
ACS Symposium Series
https://doi.org/10.1021/bk-1996-0628.ch013

Threshold dependence of laser‐induced optical breakdown on pulse duration
journal, March 1995

Niemz, M. H.
Applied Physics Letters, Vol. 66, Issue 10
https://doi.org/10.1063/1.113850

A review of the optical properties of biological tissues
journal, January 1990

Cheong, W. F.; Prahl, S. A.; Welch, A. J.
IEEE Journal of Quantum Electronics, Vol. 26, Issue 12
https://doi.org/10.1109/3.64354

The effect of spatial and temporal laser beam characteristics on two-photon absorption
journal, August 1975

Swofford, Robert L.; McClain, W. M.
Chemical Physics Letters, Vol. 34, Issue 3
https://doi.org/10.1016/0009-2614(75)85538-2

In vivo cancer diagnosis of the esophagus using differential normalized fluorescence (DNF) indices
journal, January 1995

Vo-Dinh, Tuan; Panjehpour, Masoud; Overholt, Begein F.
Lasers in Surgery and Medicine, Vol. 16, Issue 1
https://doi.org/10.1002/lsm.1900160106

Two-Photon Excitation in Ca $F_{2}$ : ${Eu}^{2 +}$
journal, September 1961

Kaiser, W.; Garrett, C. G. B.
Physical Review Letters, Vol. 7, Issue 6
https://doi.org/10.1103/PhysRevLett.7.229

Photochemistry and Photobiology of Psoralens
journal, June 1979

Song, Pill-Soon; Tapley, Kenneth J.
Photochemistry and Photobiology, Vol. 29, Issue 6
https://doi.org/10.1111/j.1751-1097.1979.tb07838.x

Molecular model for solid-state polymerization of nylon 6. II. An improved model
journal, July 1994

Kulkarni, Mukund R.; Gupta, Santosh K.
Journal of Applied Polymer Science, Vol. 53, Issue 1
https://doi.org/10.1002/app.1994.070530110

The two-photon laser-induced fluorescence of the tumor-localizing photosensitizer hematoporphyrin derivative. Resonance-enhanced 750 nm two-photon excitation into the near-UV Soret band.
journal, September 1986

Bodaness, R. S.; Heller, D. F.; Krasinski, J.
Journal of Biological Chemistry, Vol. 261, Issue 26
https://doi.org/10.1016/S0021-9258(18)67207-2

p-Bis(o-methylstyryl)benzene as a power-squared sensor for two-photon absorption measurements between 537 and 694 nm
journal, November 1986

Kennedy, S. M.; Lytle, F. E.
Analytical Chemistry, Vol. 58, Issue 13
https://doi.org/10.1021/ac00126a014

1993 Syntex Award Lecture Photomedicine and photodynamic therapy
journal, April 1994

Dolphin, David
Canadian Journal of Chemistry, Vol. 72, Issue 4
https://doi.org/10.1139/v94-129

Two-Photon Excitation Spectra of Polycyclic Aromatic Hydrocarbons
journal, November 1980

Lytle, F. E.; Hassinger, T. L.; Johnson, M. C.
International Journal of Environmental Analytical Chemistry, Vol. 8, Issue 4
https://doi.org/10.1080/03067318008071899

Very high detectability in two-photon spectroscopy
journal, May 1990

Wirth, Mary J.; Fatunmbi, H. O.
Analytical Chemistry, Vol. 62, Issue 9
https://doi.org/10.1021/ac00208a015

Second harmonic detection of sinusoidally modulated two-photon excited fluorescence
journal, October 1990

Freeman, R. Griffith.; Gilliland, Donald L.; Lytle, Fred E.
Analytical Chemistry, Vol. 62, Issue 20
https://doi.org/10.1021/ac00219a012

60-fsec pulse generation from a self-mode-locked Ti:sapphire laser
journal, January 1991

Spence, D. E.; Kean, P. N.; Sibbett, W.
Optics Letters, Vol. 16, Issue 1
https://doi.org/10.1364/OL.16.000042

Tunable dye laser pulse converter for production of picosecond pulses
journal, October 1974

Chan, C. K.; Sari, S. O.
Applied Physics Letters, Vol. 25, Issue 7
https://doi.org/10.1063/1.1655526

Clinical and preclinical photodynamic therapy
journal, January 1995

Fisher, Anita M. R.; Murphree, A. Linn; Gomer, Charles J.
Lasers in Surgery and Medicine, Vol. 17, Issue 1
https://doi.org/10.1002/lsm.1900170103

Polarization Dependence of the Two‐Photon Absorption of Tumbling Molecules with Application to Liquid 1‐Chloronaphthalene and Benzene
journal, July 1970

Monson, P. R.; McClain, W. M.
The Journal of Chemical Physics, Vol. 53, Issue 1
https://doi.org/10.1063/1.1673778

Femtosecond Dynamics of Excited-State Evolution in [Ru(bpy)3]2+
journal, January 1997

Damrauer, N. H.
Science, Vol. 275, Issue 5296
https://doi.org/10.1126/science.275.5296.54

The physics of photodynamic therapy
journal, April 1986

Wilson, B. C.; Patterson, M. S.
Physics in Medicine and Biology, Vol. 31, Issue 4
https://doi.org/10.1088/0031-9155/31/4/001

Photodynamic Therapy with a Diode Laser for Implanted Fibrosarcoma in Mice Employing Mono-L-Aspartyl Chlorin E ₆
journal, October 1996

Katsumi, Toichiro A.; Aizawa, Katsuo; Kuroiwa, Yukari
Photochemistry and Photobiology, Vol. 64, Issue 4
https://doi.org/10.1111/j.1751-1097.1996.tb03122.x

Photophysical and Photobiological Properties of Diporphyrin Ethers
journal, April 1991

Kessel, David; Byrne, Chris J.; Ward, A. David
Photochemistry and Photobiology, Vol. 53, Issue 4
https://doi.org/10.1111/j.1751-1097.1991.tb03658.x

Basic study on photochemical effect of pheophorbide a irradiated by Nd:YAG laser light [Nd: YAGレーザー光照射によるフェオフォーバイドaの光化学効果の基礎的研究]
journal, January 1986

Mashiko, Shinro; Sato, Shunichi; Inaba, Humio
THE JOURNAL OF JAPAN SOCIETY FOR LASER SURGERY AND MEDICINE, Vol. 6, Issue 3
https://doi.org/10.2530/jslsm1980.6.3_113

Two-Photon Molecular Excitation in Laser-Scanning Microscopy
book, January 1995

Denk, Winfried; Piston, David W.; Webb, Watt W.
Handbook of Biological Confocal Microscopy
https://doi.org/10.1007/978-1-4757-5348-6_28

Photodynamic therapy using pheophorbide-a and Q-switched Nd:YAG Laser on implanted human hepatocellular carcinoma
journal, October 1991

Yamashita, Yukitaka; Moriyasu, Fuminori; Ono, Shigeki
Gastroenterologia Japonica, Vol. 26, Issue 5
https://doi.org/10.1007/BF02781679

The two-photon induced fluorescence of the tumor localizing photosensitizer hematoporphyrin derivative via 1064 NM photons from a 20 NS Q-switched Nd-YAG laser
journal, January 1985

Bodaness, Richard S.; King, David S.
Biochemical and Biophysical Research Communications, Vol. 126, Issue 1
https://doi.org/10.1016/0006-291X(85)90612-6

In vivo EXCITATION OF PHOTOSENSITIZERS BY INFRARED LIGHT
journal, August 1995

Lenz, P.
Photochemistry and Photobiology, Vol. 62, Issue 2
https://doi.org/10.1111/j.1751-1097.1995.tb05277.x

Spectroscopic diagnosis of esophageal cancer: New classification model, improved measurement system
journal, June 1995

Panjehpour, Masoud; Overholt, Bergein F.; Schmidhammer, James L.
Gastrointestinal Endoscopy, Vol. 41, Issue 6
https://doi.org/10.1016/S0016-5107(95)70194-X

Photophysical properties of the photosensitizer pheophorbide a studied at high photon flux densities
journal, February 1993

Stiel, H.; Marlow, I.; Roeder, B.
Journal of Photochemistry and Photobiology B: Biology, Vol. 17, Issue 2
https://doi.org/10.1016/1011-1344(93)80011-W

Two-Photon Excitation of 4'-Hydroxymethyl-4,5',8-Trimethylpsoralen
journal, January 1997

Oh, Dennis H.; Stanley, Robert J.; Lin, Michelle
Photochemistry and Photobiology, Vol. 65, Issue 1
https://doi.org/10.1111/j.1751-1097.1997.tb01882.x

Two-photon molecular spectroscopy
journal, May 1974

McClain, W. Martin
Accounts of Chemical Research, Vol. 7, Issue 5
https://doi.org/10.1021/ar50077a001

Photoradiation Therapy. II. Cure of Animal Tumors With Hematoporphyrin and Light23
journal, July 1975

Dougherty, T. J.; Grindey, G. B.; Fiel, R.
JNCI: Journal of the National Cancer Institute, Vol. 55, Issue 1
https://doi.org/10.1093/jnci/55.1.115

Psoralens as Photoactive Probes of Nucleic Acid Structure and Function: Organic Chemistry, Photochemistry, and Biochemistry
journal, June 1985

Cimino, G. D.; Gamper, H. B.; Isaacs, S. T.
Annual Review of Biochemistry, Vol. 54, Issue 1
https://doi.org/10.1146/annurev.bi.54.070185.005443

Pulse generation in a cw dye laser by mode−locked synchronous pumping
journal, January 1975

Harris, J. M.; Chrisman, R. W.; Lytle, F. E.
Applied Physics Letters, Vol. 26, Issue 1
https://doi.org/10.1063/1.87970

Two-photon excited molecular fluorescence in optically dense media
journal, November 1977

Wirth, M. J.; Lytle, F. E.
Analytical Chemistry, Vol. 49, Issue 13
https://doi.org/10.1021/ac50021a041

Second harmonic detection of spatially filtered two-photon excited fluorescence
journal, March 1993

Fisher, Walt G.; Lytle, Fred E.
Analytical Chemistry, Vol. 65, Issue 5
https://doi.org/10.1021/ac00053a024

Properties and Applications of Photodynamic Therapy
journal, October 1989

Gomer, Charles J.; Rucker, Natalie; Ferrario, Angela
Radiation Research, Vol. 120, Issue 1
https://doi.org/10.2307/3577632

Experimental investigation of ultrashort pulse laser-induced breakdown thresholds in aqueous media
journal, April 1996

Hammer, D. X.; Thomas, R. J.; Noojin, G. D.
IEEE Journal of Quantum Electronics, Vol. 32, Issue 4
https://doi.org/10.1109/3.488842

Similar records in OSTI.GOV collections:

Method for improved selectivity in photo-activation and detection of molecular diagnostic agents

Patent Wachter, E; Fisher, W; Dees, H

A method for the imaging of a particular volume of plant or animal tissue, wherein the plant or animal tissue contains at least one photo-active molecular agent. The method includes the steps of treating the particular volume of the plant or animal tissue with light sufficient to promote a simultaneous two-photon excitation of the photo-active molecular agent contained in the particular volume of the plant or animal tissue, photo-activating at least one of the at least one photo-active molecular agent in the particular volume of the plant or animal tissue, thereby producing at least one photo-activated molecular agent, wherein themore »« less
Methods for improved selectivity in photo-activation and detection of molecular diagnostic agents

Patent Wachter, Eric; Fisher, Walter; Dees, H

A method for the imaging of a particular volume of plant or animal tissue, wherein the plant or animal tissue contains at least one photo-active molecular agent. The method comprises the steps of treating the particular volume of the plant or animal tissue with light sufficient to promote a simultaneous two-photon excitation of the photo-active molecular agent contained in the particular volume of the plant or animal tissue, photo-activating at least one of the at least one photo-active molecular agent in the particular volume of the plant or animal tissue, thereby producing at least one photo-activated molecular agent, wherein themore »« less
Full Text Available
Method for improved selectivity in photo-activation of molecular agents

Patent Fisher, W; Wachter, E; Dees, H

A method for the treatment of a particular volume of plant or animal tissue comprising the steps of treating the plant or animal tissue with at least one photo-active molecular agent, wherein the particular volume of the plant or animal tissue retains at least a portion of the at least one photo-active molecular agent, and then treating the particular volume of the plant or animal tissue with light sufficient to promote a simultaneous two-photon excitation of at least one of the at least one photo-active molecular agent retained in the particular volume of the plant or animal tissue, wherein themore »« less
Method for improved selectivity in photo-activation of molecular agents

Patent Fisher, Walter; Wachter, Eric; Dees, H

A method for the treatment of a particular volume of plant or animal tissue comprising the steps of treating the plant or animal tissue with at least one photo-active molecular agent, wherein the particular volume of the plant or animal tissue retains at least a portion of the at least one photo-active molecular agent, and then treating the particular volume of the plant or animal tissue with light sufficient to promote a simultaneous two-photon excitation of at least one of the at least one photo-active molecular agent retained in the particular volume of the plant or animal tissue, wherein themore »« less
Full Text Available
Method for improved selectivity in photo-activation of molecular agents

Patent Fisher, Walter; Wachter, Eric; Dees, H

A method for the treatment of a particular volume of plant or animal tissue comprising the steps of treating the plant or animal tissue with at least one photo-active molecular agent, wherein the particular volume of the plant or animal tissue retains at least a portion of the at least one photo-active molecular agent, and then treating the particular volume of the plant or animal tissue with light sufficient to promote a simultaneous two-photon excitation of at least one of the at least one photo-active molecular agent retained in the particular volume of the plant or animal tissue, wherein themore »« less
Full Text Available

Similar Records

Title: Method for improved selectivity in photo-activation and detection of molecular diagnostic agents

Abstract

Citation Formats

Two-step laser activation of hematoporphyrin derivative journal, April 1982

Dispersion of the two-photon cross section in rhodamine dyes journal, October 1972

A simple conceptual model for two‐photon absorption journal, January 1986

Multiphoton Spectroscopy journal, October 1967

Mechanistic investigation of doxycycline photosensitization by picosecond-pulsed and continuous wave laser irradiation of cells in culture. journal, April 1990

Über Elementarakte mit zwei Quantensprüngen journal, January 1931

A study on the possible involvement of nonlinear mechanism of light absorption by HpD with Nd: YAG laser journal, January 1986

Limitations Arising from Optical Saturation in Fluorescence and Thermal Lens Spectrometries Using Pulsed Laser Excitation: Application to the Determination of the Fluorescence Quantum Yield of Rhodamine 6G journal, December 1996

Two Photons Shine In 3-D Data Storage: Organic dyes show promise for computer memories, cancer therapy, microscopy journal, September 1996

Photodynamic Therapy Using Pheophorbide a and Nd:YAG Laser journal, January 1991

Laser Fundamentals book, January 1981

Excited State Symmetry Assignment Through Polarized Two‐Photon Absorption Studies of Fluids journal, September 1971

Multiphoton Resonant Nonlinear Optical Processes in Organic Molecules book, May 1996

Threshold dependence of laser‐induced optical breakdown on pulse duration journal, March 1995

A review of the optical properties of biological tissues journal, January 1990

The effect of spatial and temporal laser beam characteristics on two-photon absorption journal, August 1975

In vivo cancer diagnosis of the esophagus using differential normalized fluorescence (DNF) indices journal, January 1995

Two-Photon Excitation in Ca F 2 : Eu 2 + journal, September 1961

Photochemistry and Photobiology of Psoralens journal, June 1979

Molecular model for solid-state polymerization of nylon 6. II. An improved model journal, July 1994

The two-photon laser-induced fluorescence of the tumor-localizing photosensitizer hematoporphyrin derivative. Resonance-enhanced 750 nm two-photon excitation into the near-UV Soret band. journal, September 1986

p-Bis(o-methylstyryl)benzene as a power-squared sensor for two-photon absorption measurements between 537 and 694 nm journal, November 1986

1993 Syntex Award Lecture Photomedicine and photodynamic therapy journal, April 1994

Two-Photon Excitation Spectra of Polycyclic Aromatic Hydrocarbons journal, November 1980

Very high detectability in two-photon spectroscopy journal, May 1990

Second harmonic detection of sinusoidally modulated two-photon excited fluorescence journal, October 1990

60-fsec pulse generation from a self-mode-locked Ti:sapphire laser journal, January 1991

Tunable dye laser pulse converter for production of picosecond pulses journal, October 1974

Clinical and preclinical photodynamic therapy journal, January 1995

Polarization Dependence of the Two‐Photon Absorption of Tumbling Molecules with Application to Liquid 1‐Chloronaphthalene and Benzene journal, July 1970

Femtosecond Dynamics of Excited-State Evolution in [Ru(bpy)3]2+ journal, January 1997

The physics of photodynamic therapy journal, April 1986

Photodynamic Therapy with a Diode Laser for Implanted Fibrosarcoma in Mice Employing Mono-L-Aspartyl Chlorin E 6 journal, October 1996

Photophysical and Photobiological Properties of Diporphyrin Ethers journal, April 1991

Basic study on photochemical effect of pheophorbide a irradiated by Nd:YAG laser light [Nd: YAGレーザー光照射によるフェオフォーバイドaの光化学効果の基礎的研究] journal, January 1986

Two-Photon Molecular Excitation in Laser-Scanning Microscopy book, January 1995

Photodynamic therapy using pheophorbide-a and Q-switched Nd:YAG Laser on implanted human hepatocellular carcinoma journal, October 1991

The two-photon induced fluorescence of the tumor localizing photosensitizer hematoporphyrin derivative via 1064 NM photons from a 20 NS Q-switched Nd-YAG laser journal, January 1985

In vivo EXCITATION OF PHOTOSENSITIZERS BY INFRARED LIGHT journal, August 1995

Spectroscopic diagnosis of esophageal cancer: New classification model, improved measurement system journal, June 1995

Photophysical properties of the photosensitizer pheophorbide a studied at high photon flux densities journal, February 1993

Two-Photon Excitation of 4'-Hydroxymethyl-4,5',8-Trimethylpsoralen journal, January 1997

Two-photon molecular spectroscopy journal, May 1974

Photoradiation Therapy. II. Cure of Animal Tumors With Hematoporphyrin and Light23 journal, July 1975

Psoralens as Photoactive Probes of Nucleic Acid Structure and Function: Organic Chemistry, Photochemistry, and Biochemistry journal, June 1985

Pulse generation in a cw dye laser by mode−locked synchronous pumping journal, January 1975

Two-photon excited molecular fluorescence in optically dense media journal, November 1977

Second harmonic detection of spatially filtered two-photon excited fluorescence journal, March 1993

Properties and Applications of Photodynamic Therapy journal, October 1989

Experimental investigation of ultrashort pulse laser-induced breakdown thresholds in aqueous media journal, April 1996

Two-step laser activation of hematoporphyrin derivative
journal, April 1982

Dispersion of the two-photon cross section in rhodamine dyes
journal, October 1972

A simple conceptual model for two‐photon absorption
journal, January 1986

Multiphoton Spectroscopy
journal, October 1967

Mechanistic investigation of doxycycline photosensitization by picosecond-pulsed and continuous wave laser irradiation of cells in culture.
journal, April 1990

Über Elementarakte mit zwei Quantensprüngen
journal, January 1931

A study on the possible involvement of nonlinear mechanism of light absorption by HpD with Nd: YAG laser
journal, January 1986

Limitations Arising from Optical Saturation in Fluorescence and Thermal Lens Spectrometries Using Pulsed Laser Excitation: Application to the Determination of the Fluorescence Quantum Yield of Rhodamine 6G
journal, December 1996

Two Photons Shine In 3-D Data Storage: Organic dyes show promise for computer memories, cancer therapy, microscopy
journal, September 1996

Photodynamic Therapy Using Pheophorbide a and Nd:YAG Laser
journal, January 1991

Laser Fundamentals
book, January 1981

Excited State Symmetry Assignment Through Polarized Two‐Photon Absorption Studies of Fluids
journal, September 1971

Multiphoton Resonant Nonlinear Optical Processes in Organic Molecules
book, May 1996

Threshold dependence of laser‐induced optical breakdown on pulse duration
journal, March 1995

A review of the optical properties of biological tissues
journal, January 1990

The effect of spatial and temporal laser beam characteristics on two-photon absorption
journal, August 1975

In vivo cancer diagnosis of the esophagus using differential normalized fluorescence (DNF) indices
journal, January 1995

Two-Photon Excitation in Ca $F_{2}$ : ${Eu}^{2 +}$
journal, September 1961

Photochemistry and Photobiology of Psoralens
journal, June 1979

Molecular model for solid-state polymerization of nylon 6. II. An improved model
journal, July 1994

The two-photon laser-induced fluorescence of the tumor-localizing photosensitizer hematoporphyrin derivative. Resonance-enhanced 750 nm two-photon excitation into the near-UV Soret band.
journal, September 1986

p-Bis(o-methylstyryl)benzene as a power-squared sensor for two-photon absorption measurements between 537 and 694 nm
journal, November 1986

1993 Syntex Award Lecture Photomedicine and photodynamic therapy
journal, April 1994

Two-Photon Excitation Spectra of Polycyclic Aromatic Hydrocarbons
journal, November 1980

Very high detectability in two-photon spectroscopy
journal, May 1990

Second harmonic detection of sinusoidally modulated two-photon excited fluorescence
journal, October 1990

60-fsec pulse generation from a self-mode-locked Ti:sapphire laser
journal, January 1991

Tunable dye laser pulse converter for production of picosecond pulses
journal, October 1974

Clinical and preclinical photodynamic therapy
journal, January 1995

Polarization Dependence of the Two‐Photon Absorption of Tumbling Molecules with Application to Liquid 1‐Chloronaphthalene and Benzene
journal, July 1970

Femtosecond Dynamics of Excited-State Evolution in [Ru(bpy)3]2+
journal, January 1997

The physics of photodynamic therapy
journal, April 1986

Photodynamic Therapy with a Diode Laser for Implanted Fibrosarcoma in Mice Employing Mono-L-Aspartyl Chlorin E ₆
journal, October 1996

Photophysical and Photobiological Properties of Diporphyrin Ethers
journal, April 1991

Basic study on photochemical effect of pheophorbide a irradiated by Nd:YAG laser light [Nd: YAGレーザー光照射によるフェオフォーバイドaの光化学効果の基礎的研究]
journal, January 1986

Two-Photon Molecular Excitation in Laser-Scanning Microscopy
book, January 1995

Photodynamic therapy using pheophorbide-a and Q-switched Nd:YAG Laser on implanted human hepatocellular carcinoma
journal, October 1991

The two-photon induced fluorescence of the tumor localizing photosensitizer hematoporphyrin derivative via 1064 NM photons from a 20 NS Q-switched Nd-YAG laser
journal, January 1985

In vivo EXCITATION OF PHOTOSENSITIZERS BY INFRARED LIGHT
journal, August 1995

Spectroscopic diagnosis of esophageal cancer: New classification model, improved measurement system
journal, June 1995

Photophysical properties of the photosensitizer pheophorbide a studied at high photon flux densities
journal, February 1993

Two-Photon Excitation of 4'-Hydroxymethyl-4,5',8-Trimethylpsoralen
journal, January 1997

Two-photon molecular spectroscopy
journal, May 1974

Photoradiation Therapy. II. Cure of Animal Tumors With Hematoporphyrin and Light23
journal, July 1975

Psoralens as Photoactive Probes of Nucleic Acid Structure and Function: Organic Chemistry, Photochemistry, and Biochemistry
journal, June 1985

Pulse generation in a cw dye laser by mode−locked synchronous pumping
journal, January 1975

Two-photon excited molecular fluorescence in optically dense media
journal, November 1977

Second harmonic detection of spatially filtered two-photon excited fluorescence
journal, March 1993

Properties and Applications of Photodynamic Therapy
journal, October 1989

Experimental investigation of ultrashort pulse laser-induced breakdown thresholds in aqueous media
journal, April 1996