Prolonged cold storage of red blood cells by oxygen removal and additive usage

Patent · Thu Jan 01 00:00:00 EST 1998

OSTI ID:871751

Bitensky, Mark W ^[1]; Yoshida, Tatsuro ^[2]

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1. Boston, MA
2. Newton, MA

Prolonged cold storage of red blood cells by oxygen removal and additive usage. A cost-effective, 4.degree. C. storage procedure that preserves red cell quality and prolongs post-transfusion in vivo survival is described. The improved in vivo survival and the preservation of adenosine triphosphate levels, along with reduction in hemolysis and membrane vesicle production of red blood cells stored at 4.degree. C. for prolonged periods of time, is achieved by reducing the oxygen level therein at the time of storage; in particular, by flushing the cells with an inert gas, and storing them in an aqueous solution which includes adenine, dextrose, mannitol, citrate ion, and dihydrogen phosphate ion, but no sodium chloride, in an oxygen-permeable container which is located in an oxygen-free environment containing oxygen-scavenging materials.

• Research Organization: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
• DOE Contract Number: W-7405-ENG-36
• Assignee: Regents of University of California (Los Alamos, NM)
• Patent Number(s): US 5789151
• OSTI ID: 871751
• Country of Publication: United States
• Language: English

Related Subjects

prolonged
cold
storage
blood
cells
oxygen
removal
additive
usage
cost-effective
degree
procedure
preserves
cell
quality
prolongs
post-transfusion
vivo
survival
described
improved
preservation
adenosine
triphosphate
levels
reduction
hemolysis
membrane
vesicle
production
stored
periods
time
achieved
reducing
level
therein
particular
flushing
inert
gas
storing
aqueous
solution
adenine
dextrose
mannitol
citrate
dihydrogen
phosphate
sodium
chloride
oxygen-permeable
container
located
oxygen-free
environment
containing
oxygen-scavenging
materials
cold storage
environment containing
sodium chloride
blood cells
aqueous solution
inert gas
containing oxygen
blood cell
prolonged period
oxygen removal
prolonged periods
storage procedure
cell quality
hydrogen ph
prolonged cold
additive usage
/435/604/