Dyes designed for high sensitivity detection of double-stranded DNA

Patent · Thu Jan 01 00:00:00 EST 1998

OSTI ID:871730

Glazer, Alexander N ^[1]; Benson, Scott C ^[2]

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1. Orinda, CA
2. Oakland, CA

Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.

• Research Organization: Univ. of California (United States)
• DOE Contract Number: FG03-91ER61125
• Assignee: Regents of University of California (Berkeley, CA)
• Patent Number(s): US 5783687
• OSTI ID: 871730
• Country of Publication: United States
• Language: English

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designed
sensitivity
detection
double-stranded
dna
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fluorophore
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cationic
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nucleic
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strong
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compared
polycationic
dsdna
electrophoresis
solution
substantially
sensitivities
dye
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stranded dna
dyes designed
double-stranded dna
nucleic acid
fluorescent dyes
fluorescent dye
sensitivity detection
strong binding
binding affinities
polycationic chain
cationic chain
fluorophore joined
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