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Title: Recombinant Zymomonas for pentose fermentation

Patent ·
OSTI ID:871411
 [1];  [2];  [3];  [4]
  1. Golden, CO
  2. Lakewood, CO
  3. Saratoga Springs, NY
  4. Conifer, CO
+ Show Author Affiliations

The invention relates to microorganisms which normally do not ferment pentose sugar and which are genetically altered to ferment pentose sugar to produce ethanol, and fermentation processes utilizing the same. Examples include Zymomonas mobilis which has been transformed with combinations of E. coli genes for xylose isomerase, xylulokinase, transaldolase, transketolase, L-arabinose isomerase, L-ribulokinase, and L-ribulose-5-phosphate 4-epimerase. Expression of the added genes are under the control of Zymomonas mobilis promoters. These newly created microorganisms are useful for fermenting pentoses and glucose, produced by hydrolysis of hemicellulose and cellulose, to produce ethanol.

Research Organization:
Midwest Research Institute, Kansas City, MO (United States)
DOE Contract Number:
AC36-83CH10093
Assignee:
Midwest Research Institute (Kansas City, MO)
Patent Number(s):
US 5726053
OSTI ID:
871411
Country of Publication:
United States
Language:
English

References (7)

The organization of the araBAD operon of Escherichia coli journal January 1986
Pentose metabolism in Zymomonas mobilis wild-type and recombinant strains journal December 1992
Approaches to broaden the substrate and product range of the ethanologenic bacterium Zymomonas mobilis by genetic engineering journal February 1993
Molecular characterization of the Zymomonas mobilis enolase (eno) gene. journal January 1992
Glyceraldehyde-3-phosphate dehydrogenase gene from Zymomonas mobilis: cloning, sequencing, and identification of promoter region. journal December 1987
Systematic sequencing of the Escherichia coli genome: analysis of the 0 – 2.4 min region journal January 1992
Expression of cloned Xanthomonasd-xylose catabolic genes in Zymomonas mobilis journal January 1988

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Related Subjects

recombinant
zymomonas
pentose
fermentation
relates
microorganisms
normally
ferment
sugar
genetically
altered
produce
ethanol
processes
utilizing
examples
mobilis
transformed
combinations
coli
genes
xylose
isomerase
xylulokinase
transaldolase
transketolase
l-arabinose
l-ribulokinase
l-ribulose-5-phosphate
4-epimerase
expression
added
control
promoters
newly
created
useful
fermenting
pentoses
glucose
produced
hydrolysis
hemicellulose
cellulose
xylose isomerase
pentose sugar
fermentation process
zymomonas mobilis
genetically altered
produce ethanol
recombinant zymomonas
pentose fermentation
coli genes
newly created
mobilis promoters
ferment pentose
fermentation processes
added genes
fermenting pentoses
created microorganisms
l-arabinose isomerase
processes utilizing
/435/999/